Nikon Eclipse Ni-E Instruction Manual page 160

Photomicroscopy
The photomicroscopy procedure is described below. See the instruction manual bundled with the DS-U3 or DS-L3, or the
instruction manual for the microscope's setup/control software "Ni Setup Tool" for details including the camera settings.
Using DS-L3
You must choose at least the following information:
 Folder for data storage.
 Name of the file to be saved. (Or select "Auto".)
 File format and file size.
 Date and destination of data
(1) Adjust the illumination of the microscope correctly, and
adjust the focus onto the specimen image.
(2) Adjust the DS camera head attachment position.
For details on the camera head attachment, see Chapter 1
"3 Assembly Method - 23 Attach a camera and connect the
DS camera control unit" in the "Assembly/Maintenance"
instruction manual.
 DSC zooming port for quadrocular tube
Loosen the attachment direction fixing screw for the C
mount so that an image moves in the reverse direction
when the stage is moved right and left, adjust the camera
position, and then tighten the screw to fix it.
 DSC port for ergonomic binocular tube
Align the camera head attachment position with the stage
moving direction. Loosen the attachment direction fixing
screw for the C mount so that an image moves in the reverse
direction when the stage is moved right and left, adjust the
camera position, and then tighten the screw to fix it.
(3) Focus the image.
If the image viewed through the eyepiece appears to be in
focus but the image on the monitor is out of focus, make
adjustment.
 DSC zooming port for quadrocular tube
Adjust the position of the internal lenses from the rear of
the zooming port by rotating the focal position adjustment
screw using a hex wrench to focus it.
 DSC port for ergonomic binocular tube
Rotate the camera fine focus adjustment ring for the C
mount to adjust the focus onto the image in the monitor,
and then rotate the camera centering screws on the right
and left to align the position of the image viewed through
the eyepiece with the image on the monitor.
In both ports, if diopter is not properly adjusted, the same
phenomenon occurs. Be sure to make diopter adjustment
as well. (See Chapter 3 "6 Adjusting the Diopter".)
(4) Select the camera scene mode suitable for the
microscopy method.
Chapter 3
Individual Operations
Adjusting the camera position for the DSC zooming
Focal position
adjustment
screw
Camera and focal position adjustment for the DSC
146
Camera head
port
Focusing the image using a DSC zooming port
Attachment direction
fixing screw
port for ergonomic binocular tube
C mount
attachment
direction fixing
screw
Camera head
C mount
Camera fine focus
adjustment ring
Camera centering
screw (x2)