Nikon Eclipse Ni-E Instruction Manual page 53

10
Adjust the field diaphragm of the epi-fluorescence attachment.
Use the field diaphragm lever of the epi-fluorescence
attachment to adjust the field diaphragm so that it
circumscribes the field of view.
Size of the field diaphragm
Normally, adjust the field diaphragm so that it
circumscribes the field of view. Excessively opening
the field diaphragm will result in stray light entering
the field of view, generating flare and reducing the
image contrast. In addition, the sample will become
decolorized over a wider area.
Field diaphragm's adjustment timing
Be sure to adjust the field diaphragm each time you
change the objective.
11
Adjust the brightness.
Use ND filters on the epi-fluorescence attachment to
adjust image brightness.
The aperture diaphragm of the epi-fluorescence
attachment can also be used for brightness adjustment
of the image. Be sure to center the aperture diaphragm
before use. (See Chapter 3 "16.4 Adjusting the
Brightness of the Fluorescent Image (Using ND Filters
and the Aperture Diaphragm)".)
Chapter 2
Microscopy Operations
Preparation
Epi-fluorescence
Microscopy Procedure
1 2 3 4 5
39
Focusing Microscopy
6 7 8 9 10 11 12 13 14 ■ ■ ■ ■ ■
Field diaphragm
Circumscribe around the field of view.
Appropriate size of the field diaphragm
Adjusting the field diaphragm
Brightness adjustment using the ND filter
Field of view