Nikon Eclipse Ni-E Instruction Manual page 40

For dark-field microscopy, set the condenser turret to the [DF] (dark-field module)
position (this step is not required for bright-field microscopy).
Press the FUNCTION button 1/2 to turn the condenser
turret and display [DF] on the front panel of Ni-E. The
turret's dark-field module enters the optical path in this
state.
Button 1 is for counterclockwise rotation and button 2 is
for clockwise rotation.
Dark-field module
The dark-field module can be used with any objective
with a numerical aperture (NA) of 0.7 or less. Note,
however, that 2x and 4x objectives are not supported.
2x and 4x objectives can only be used for bright-field
microscopy.
Chapter 2
Microscopy Operations
Preparation
Bright/Dark-field
Microscopy
1 2 3 4 5 6 7
26
Focus and Optical Micro-
System Adjustment
scopy
8 9 10 11 12 13 14 15 16 17 18 19
Optical
module
in optical
path [DF]
Bring the dark-field module into the optical path
P A _ _ 2 x _ _ _ _ Z :
D F _ _ _ _ A S 3 0 . 0 m m
[CONDEN.] button, sub screen: [DF]
NC B
ND
ND
11
32
8
OU T
IN
_ _ _ _ _ 0 . 0 0 0 u m
_ _ F S 3 0 . 0 m m