Nikon Eclipse Ni-E Instruction Manual page 130

Water immersion
An objective with a "WI" or "W" marking is a water-immersion objective that can focus on the sample at an operating
distance under water. This objective is used by filling the chamber with water (distilled water or physiological saline) and
dipping the tip of the objective into the water.
Since water evaporates readily, monitor the immersion water during observation. Avoid filling the chamber with too much
water or spilling the water, since excess water will flow onto the stage and around the condenser, promoting corrosion. In
such cases, immediately wipe off the water.
Cleaning of water
After use, wipe off the water from the tip of the objective and condenser, then follow up by wiping with absolute alcohol.
If you observe water stains, apply a small amount of neutral detergent and wipe gently, and then follow up with absolute
alcohol.
CAUTION
 Absolute alcohol used for cleaning off the immersion water is highly flammable. Be careful when handling these
materials, particularly around open flames or when turning the power switch on or off.
 Follow the instructions provided by the manufacturer when using absolute alcohol.
Chapter 3
Individual Operations
116