Nikon Eclipse Ni-E Instruction Manual page 58

Microscopy Procedure
(4) When differential interference contrast microscopy is
finished, go to epi-fluorescence microscopy.
• Press the dia-illumination ON/OFF switch to turn off
the lamp and make the diascopic image disappear.
• Rotate the epi-fluorescence cube turret to bring the
desired filter cube into the optical path.
• Remove the FL/DIC analyzer slider from optical
path.
• Fully open the field diaphragm and aperture
diaphragm of the epi-fluorescence attachment.
(5) Turn on the power of the HG precentered fiber
illuminator and continue from procedure 6 in the
epi-fluorescence microscopy procedure.
Chapter 2
Microscopy Operations
Preparation
Epi-fluorescence
1 2 3 4 5 6 7
44
Focusing Microscopy
8 9 10 11 12 13 14 ■ ■ ■ ■ ■
Dia-illumination lamp OFF
Bringing the filter cube into the optical path
NI-FA FL/DIC
Analyzer Slider
2
3
4
5
Removing the FL/DIC analyzer slider from optical path
Field diaphragm
lever
Fully opening field and aperture diaphragms
ND ND NCB
8 32 11
OUT
IN
F. ST OP
6
A. ST OP
F. ST OP
Aperture
diaphragm lever