Nikon Eclipse Ni-E Instruction Manual page 71

Differential Interference
Contrast Microscopy
11
Perform diopter adjustment.
Look into the right eyepiece with your right eye and the
left eyepiece with your left eye. Turn the diopter
adjustment ring of each eyepiece to focus on the
specimen. Do not use the focus knobs. (See Chapter 3
"6 Adjusting the Diopter".)
12
Adjust the interpupillary distance.
Look into both eyepieces and rotate the binocular part to
adjust the binocular part's opening until the fields of view
for the right and left eyes coincide.
Tip on adjusting the interpupillary distance
For easy adjustment, look into the eyepiece as if you
were looking at a distant object.
13
Focus and center the condenser.
(1) Press the DIA field diaphragm button to close the field
diaphragm to a minimum and look into the eyepiece.
Focus on the field diaphragm image using the
condenser focus knob, and then adjust the condenser
centering screws with a tool to center the diaphragm
image within the field of view.
(2) Operate the nosepiece to bring the 40x objective into
the optical path. (With the condenser turret at [OPEN]
empty position.) If DIC slider at objective 40x position is
in the optical path, pull it out and remove it from optical
path.
(3) Check the focus and centering of the field diaphragm
image and adjust if necessary.
(4) Press the DIA field diaphragm button and adjust the
field diaphragm image so that its size is close to the
field of view.
(See Chapter 3 "7 Focusing and Centering the
Condenser".)
Chapter 2
Microscopy Operations
Preparation
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 ■
57
Focus and Optical
Microscopy
System Adjustment
Adjusting the diopter
Adjusting the interpupillary distance
Field diaphragm
image
Eyepiece
field ring
Focusing and centering the condenser
■
Coincide the field
of view for the
right and left
Field diaphragm
image
Eyepiece
field ring