Nikon Eclipse Ni-E Instruction Manual page 94

Microscopy Procedure
19
Adjust the field diaphragm.
Press the DIA field diaphragm button to adjust the field
diaphragm so that it circumscribes the field of view.
Size of the field diaphragm
Normally, the field diaphragm should be adjusted so
that it circumscribes the field of view.
opening the field diaphragm will result in stray light
entering the field of view, generating flare and
reducing the image contrast. It will also cause the
specimen to become decolorized over a wider area.
Adjustment timing for the field diaphragm
Be sure to adjust the field diaphragm each time you
change the objective.
Chapter 2
Microscopy Operations
Preparation
Phase Contrast
1 2 3 4 5 6 7 8
Excessively
80
Focus and Optical
Microscopy
System Adjustment
9 10 11 12 13 14 15 16 17 18 19 20 21 22 ■
Adjusting field diaphragm
P A _ 1 0 x _ _ _ _
P h 2 _ _ A S 1 2 . 3 m m
Field diaphragm
Circumscribe around the field of view.
Appropriate size of the field diaphragm
[F. STOP] button,
sub screen: DIA field diaphragm adjustment
■
ND ND NCB
8 32 11
OUT
IN
Z : _ _ 1 2 3 5 . 5 5 5 u m
_ _ _ F S 1 5 . 5 m m
Field of view