Nikon Eclipse Ni-E Instruction Manual page 56

Switch from Epi-Fluorescence Microscopy to Bright-field Microscopy
(1) When switching from epi-fluorescence to bright-field
microscopy, close the epi-fluorescence illumination path
shutter and block the illumination of the mercury lamp.
The shutter is closed when the [FL SHUTTER] button is
lit.
(2) Rotate the epi-fluorescence cube turret to bring turret
address 1 into the optical path. (If a filter cube is in
address 1, remove it in advance.)
(3) Press the dia-illumination ON/OFF switch to turn on the
lamp.
(4) Focus and center the condenser. (See Chapter 3 "7
Focusing and Centering the Condenser".)
For details on other operations and precautions, see "3
Bright/Dark-field Microscopy Procedure".
Chapter 2
Microscopy Operations
Preparation
Epi-fluorescence
Microscopy Procedure
1 2 3 4 5
42
Focusing Microscopy
6 7 8 9 10 11 12 13 14 ■ ■ ■ ■ ■
Lit
Closing shutter
Removing the filter cube
P F _ _ 2 x _ _ _ _
O P E N C _ _ _ _ _
'
Empty position Shutter closed
Dia-illumination lamp ON
ND ND NCB
8 32 11
OUT
IN
ND ND NCB
8 32 11
OUT
IN
Z : _ _ _ _ _ 0 . 0 0 0 u m
N D _ 1 C _ _ E P I : C
'