Nikon Eclipse Ni-E Instruction Manual page 75

Differential Interference
Contrast Microscopy
18
Adjust the aperture diaphragm
Press the DIA aperture diaphragm button to adjust the
aperture. The aperture should be adjusted to about 70 to
80% of the numerical aperture of the objective. (See
Chapter 3 "8 Adjusting the Aperture Diaphragm".)
Appropriate size of the aperture
diaphragm
Normally, the appropriate size of the aperture
diaphragm is 70 to 80% of the numerical aperture of
the objective. Since an excessively small aperture
diaphragm opening will degrade the image
resolution, Nikon does not recommend setting the
aperture diaphragm to less than 60% of the
numerical aperture of the objective.
Adjustment timing for the aperture
diaphragm
Be sure to adjust the aperture diaphragm each time
you change the objective.
19
Focus on the sample
Focus on the sample by rotating the focus knob. (See
Chapter 3 "4.1 Proper Focusing Procedure".)
Chapter 2
Microscopy Operations
Preparation
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 ■
61
Focus and Optical
Microscopy
System Adjustment
Adjusting aperture diaphragm
P A _ 4 0 x _ _ _ _ Z : _ _
N 1 _ _ _ A S 1 2 . 8 m m
Pupil of objective
Appropriate size of the aperture diaphragm
[A. STOP] button, sub screen: DIA aperture
diaphragm adjustment
Focusing using the focus knob
P A _ 4 0 x _ _ _ _ Z : _ _
N 1 _ _ _ A S 1 2 . 8 m m
Z-axis coordinate
■
ND ND NCB
8 32 11
OUT
IN
1 2 3 4 . 5 6 7 u m
_ _ _ F S 3 0 . 0 m m
Aperture
diaphragm image
70～80
100
1 2 3 5 . 5 5 5 u m
_ _ _ F S 3 0 . 0 m m