Nikon Eclipse Ni-E Instruction Manual page 95

Microscopy Procedure
20
Adjust the brightness.
Adjust the brightness of the image using the ND filter.
Press the ND filter IN/OUT switch to the [IN] (lower) side
to bring the filter into the optical path.
Brightness can be adjusted by rotating the
dia-illumination brightness control knob, note, however,
that the image color could be changed. (See Chapter 3
"3 Adjusting the Brightness of a Diascopic Image".)
Photomicrography voltage setting: for
improved color reproduction
Changing the lamp voltage for brightness adjustment
will affect the lamp color temperature and alter the
color balance of the image. Increasing the voltage
will result in a bluish tint, while reducing the voltage
results in a reddish tint. If accurate color reproduction
is critical, set the dia-illumination lamp brightness
control knob at the
brightness to the photomicrography voltage suitable
for imaging.
(Photomicrography voltage value is adjusted and set
at shipment and users cannot modify it.) To adjust the
brightness further without changing color, bring the
NCB11 filter in the filter cassette into the optical path.
Use the ND filters for adjustment. (See Chapter 3 "3
Adjusting the Brightness of a Diascopic Image".)
Viewing the specimen
21
View the specimen.
To observe another part of the sample, rotate the stage
knobs to find the position to observe. If the sample is not
in focus, rotate the focus knobs to adjust the focus.
To observe under a different magnification, repeat steps
15 and later with another objective.
NI-FA FL/DIC Analyzer Slider/Dummy Slider
When the epi-fluorescence cube turret is attached to
the microscope, the optical element for the
epi-fluorescence microscopy installed in the
epi-fluorescence cube turret is not used in phase
contrast microscopy. Be sure to bring Address 1
(empty) on the turret into the optical path.
When the FL/DIC analyzer slider is inserted in the
epi-fluorescence cube turret, pull it out to the first click
position to remove the analyzer from the optical path.
When a dummy slider instead of the analyzer slider is
inserted, do not fully insert it. Pull it out one step. If it
is fully inserted, the periphery of field of view may be
dark.
Chapter 2
Microscopy Operations
Preparation
Phase Contrast
1 2 3 4 5 6 7 8
mark to adjust the lamp
81
Focus and Optical
Microscopy
System Adjustment
9 10 11 12 13 14 15 16 17 18 19 20 21 22 ■
Brightness adjustment with ND filter
Photomicrography voltage setting
■
ND ND NCB
8 32 11
OUT
IN
Photomicrography
voltage mark