Adjusting The Aperture Diaphragm (As) - Olympus GX53 Instructions Manual

3-10 Adjusting the aperture diaphragm (AS)

a
c
26
1
2
b
3
4
NOTE
Slide the BF/DF selection lever to set to the BF side.
Rotate the revolving nosepiece to engage the 10X objective in the light
path and bring the sample into focus approximately.
If you remove the eyepieces and look into the observation tube, you
can see the aperture diaphragm image. Pull the aperture diaphragm
lever a to adjust so that the aperture diaphragm image becomes
approximately 70% of the diameter of the pupil of the objective (as
shown in the picture on the left).
Aperture diaphragm image Aperture diaphragm image
Pupil of objective Pupil of objective
· If the center of the aperture diaphragm is deviated from the
TIP
center of field, insert the Allen screwdriver into both AS centering
screws b (2 positions) and rotate them to adjust it to come to
the center of the field of view.
· Attaching the AS extension lever c provided with microscope
frame allows you to operate AS easily.
Return the eyepieces back to the observation tube. While looking at
the observed image, rotate the aperture diaphragm ring and fine-adjust
it to obtain the desired image.
In case of reflected light darkfield observation, push in the
aperture diaphragm lever a to fully open the aperture
diaphragm. If the aperture diaphragm is not fully opened,
the observed image becomes dark and the darkfield
observation may not be available.
Depending on samples to be observed, you can obtain the
TIP
image with high contrast and few flare by slightly narrowing
down the aperture diaphragm in some cases.
70%
30%