Adjusting The Aperture Diaphragm (As) - Olympus BXC-CBB Instructions Manual

4.3 Adjusting the aperture diaphragm (AS)

NOTE:
If you do not combine the eyepieces, the standard straight tube and the centering telescope are necessary. Contact us for
assistance.
1.
Select BF.
2.
Bring the sample into focus.
3.
When you remove the eyepieces and look into the observation tube, you can see the image of aperture diaphragm.
Send the command so that the image of aperture diaphragm becomes approx. 70% of the diameter of the pupil of
objective (as shown in the picture below). For details on the commands, refer to the command reference manual.
TIP:
The optimum pulse numbers for typical objectives are listed below.
AS recommended
Objective pulse number
MPLFLN1.25X
MPLFLN2.5X2
MPLFLN2.5X2BD
MPLFLN5X2
MPLFLN5X2BD2
MPLFLN10X
MPLFLN10XBD
MPLFLN20X
MPLFLN20XBD
MPLFLN50X
MPLFLN50XBD
4. Operation procedures
Objective
[pulses]
MPLFLN100X
1571
MPLFLN100XBD
1571 MPLFLN150XBD
LMPLFLN5X
1465
LMPLFLN5XBD
LMPLFLN10X
1465
LMPLFLN10XBD
LMPLFLN20X
1071
LMPLFLN20XBD
LMPLFLN50X
729
LMPLFLN50XBD
AS recommended
pulse number Objective
[pulses]
LMPLFLN100X
361
LMPLFLN100XBD
204 MPLAPON50X
1255 MPLAPON100X
1203 MXPLFLN20X
940 MXPLFLN20XBD
MXPLFLN50X
414
MXPLFLN50XBD
4.3 Adjusting the aperture diaphragm (AS)
AS recommended
pulse number
[pulses]
309
887
388
1465
1334
729
28