Multi-Channel Performance - Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual

Data collection rate
• Data can be generated with a maximum collection rate of 100 Hz or 200 Hz
(FLD-3400RS under Chromeleon 7.1 or later). The LongLife lamp mode supports a
maximum data collection rate of 20 Hz.
• In general, each peak should be defined by at least 20 data points. For chromatograms
with co-eluting peaks or low signal-to-noise ratios, 40 data points per peak is
recommended.
• If all peaks are relatively wide, select a slower data collection rate (for example, 1.0 Hz).
This saves disk space and allows for a faster display of data in Chromeleon.
• If the data collection rate is too slow, the start points and end points of peaks will not be
determined accurately.
• If any peaks of interest are less than a few seconds, select a faster data collection rate
(10.0 Hz, for example). If the collection rate is too high, data files may need more disk
space and post-run analyses may require more processing time.

5.7.5 Multi-Channel Performance

In multiple wavelength applications, baseline noise increases at higher data collection
rates. The maximum data collection rate depends on a number of parameters, for example,
the number of recorded channels and the wavelength ranges. Therefore, in Multi-Channel
Mode, the Data Collection Rate and Response Time are always automatically calculated on
the basis of the selected parameters. However, you can select the measuring performance
(Multi-Channel Performance). Determine whether the measurement is performed faster,
but with more noise, or whether minimum noise is required, which means a longer
measuring time and thus a lower Data Collection Rate. The possible values are: UltraFast,
Fast, Standard, LowNoise, UltraLowNoise.
Use the Program Wizard to set the measuring performance for all channels (→ page 68) if
you have selected multiple channels.
Operating Instructions
Fluorescence Detectors FLD-3100 and FLD-3400RS
UltiMate 3000 Series:
Page 87