Scans (Only Chromeleon 7.1 Or Later) - Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual

UltiMate 3000 Series:
Fluorescence Detectors FLD-3100 and FLD-3400RS
Complete the following steps to record an emission spectrum:
1. Open the Commands dialog box for the detector. Under ScanStartEmWavelength,
enter the first wavelength of the range to be scanned, and the last wavelength under
ScanEndEmWavelength. Select an excitation wavelength
(ScanStartExWavelength) and a scan speed (ScanSpeed).
2. Stop the pump flow (Flow=0) or set it to a very low flow rate.
3. Execute the ScanEmission command.
Complete the following steps to perform a Synchro Scan:
1. Open the Commands dialog box for the detector. Under ScanStartExWavelength,
enter the first wavelength of the range to be scanned, and the last wavelength under
ScanEndExWavelength. Then enter the offset between the scanned wavelengths
under ScanSyncOffset and select a scan speed (ScanSpeed).
2. Stop the pump flow (Flow=0) or set it to a very low flow rate.
3. Execute the ScanSynchronous command to run a synchro scan.
5.6.5 3D Scans (only Chromeleon 7.1 or later)
From Chromeleon 7.1 on, 3D scanning is supported for the FLD to facilitate determining
the retention times and absorption maxima. As opposed to 2D scans, where the selected
spectral range is scanned once, the spectral range is scanned continuously, resulting in a
spectral field similar to a 3D field of a diode array detector. As for 2D scans, 3D scan
modes include excitation scan, emission scan, and synchro scan.
Instrument methods must be used for recording the spectra. In the Instrument Method Wizard,
select the 3D Data Acqusition on option. All required parameters can be set on the FL Field
Settings page of the wizard. During a 3D scan, no emission channels can be recorded.
The recorded 3D data are represented as a 3D data field in the Contour Plot. Use the 3D data
field to determine the retention times and optimum emission or excitation wavelengths.
For more information about 3D data fields, see the Chromeleon 7.1 (or later) Help.
Page 76 Operating Instructions