Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual page 26

UltiMate 3000 Series:
Fluorescence Detectors FLD-3100 and FLD-3400RS
No. Component
1 Xenon lamp
2 Lamp optics
3 Excitation
monochromator
4 Flow cell
5 Reference detector
6 Emission optics
7 Filter wheel
8 Emission
monochromator
9 PMT
10 Second PMT
Page 18
Description
Light source for the UV to near-infrared wavelength range
Receives the light beam emitted from the xenon lamp and focuses it so
that the beam passes through the excitation monochromator.
Lets only light with the selected excitation wavelength pass.
The eluent with the analyte travels through the flow cell. The excitation
light passes through the flow cell to the reference detector, the
fluorescence light exits the flow cell at an angle of 90° to the excitation
light.
Measures the excitation light intensity though the flow cell.
Receives the light beam emitted from the flow cell and focuses it so that
the beam passes through the emission monochromator.
Carries the optical filter, which is used to cut off light up to a certain
wavelength. The FLD-3400RS provides five filter positions with
different cut-off wavelengths.
Lets only light with the selected emission wavelength pass.
Photomultiplier tube (PMT) - Converts the emitted light to a
measureable current signal.
(Optional) A second red-sensitive photomultiplier tube measures light in
the near infrared region (up to 900 nm).
Operating Instructions