Overview; Unit Description - Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual

2 Overview

2.1 Unit Description

The detector is a high-quality instrument designed for HPLC analysis as part of the
UltiMate 3000 system.
• A xenon flash lamp is used to exite a wavelength range of 200 to 880 nm.
• With the unique Dual-PMT option, the wavelength range can be extended to the near
infrared spectral region (up to 900 nm) without any loss in sensitivity in the UV/VIS
spectral region.
• Data collection rates in single channel mode of up to 100 Hz (FLD-3100) or up to 200 Hz
(FLD-3400RS under Chromeleon 7) allow rapid separations in UHPLC. The wavelength
switching time is < 250 ms.
• The detector is optimized for high sensitivity (signal-to-noise ratio higher than 550 for
the Raman spectrum of water), over the entire lifetime of the lamp. It is thus possible to
detect even smallest peaks.
• The flow cells are equipped with an active temperature control to ensure improved
reproducibility when ambient temperatures fluctuate.
• To suppress the higher-order radiation typical of grating spectrometers and scattering of
light, the detector is equipped with an optical filter. Users can select the wavelength range
that is suppressed by the filter for FLD-3400RS detectors.
• The FLD-3400RS can measure up to four channels (2D data) with independent
parameters (wavelengths, sensitivity, filter wheel) simultaneously.
• The detector can be controlled by the Chromeleon Chromatography Management
System. This provides a high degree of system integration, as well as maximum analysis
efficiency due to comprehensive data analysis and evaluation features in Chromeleon.
• Various safety and monitoring features are provided for optimum system performance
and reliability (→ page 27).
• All parts that may be exposed to solvents are made of materials that provide optimum
resistance to the most commonly used solvents and buffer solutions in HPLC.
Operating Instructions
Fluorescence Detectors FLD-3100 and FLD-3400RS
UltiMate 3000 Series:
Page 15