Installing The Flow Cell - Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual

UltiMate 3000 Series:
Fluorescence Detectors FLD-3100 and FLD-3400RS

4.4 Installing the Flow Cell

Observe the following when installing a flow cell:
• Always hold flow cells by the flow cell body with the type label. Do not touch the optical
block of the flow cell (→ Fig. 15) or the sensitive electronics on the flow cell rear side.
• A cover is mounted on the flow cell opening when the detector is shipped. Keep this
cover in a safe place. If the detector is shipped or shut down for a longer time period,
reinstall the cover.
• Plugs are installed at the flow cell in and out connections (→ Fig. 19). Keep the plugs in
a safe place. If the detector or the cell is shipped or if the detector is shut down for a
longer time period, reinstall the plugs.
• Capillary connections between the column and flow cell should be as short as possible to
avoid peak broadening effects due to excessive dead volume.
• When connecting the flow cells, make sure not to invert input and output. This will
deteriorate the measured results and may destroy the flow cell due to the increased
backpressure in the cell.
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Fig. 15: Flow cell of the fluorescence detector
Optical block
Flow cell body
Operating Instructions