Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual page 99

To perform wavelength verification
You can perform wavelength verification via Chromeleon. Wavelength verification can
take a few minutes. During this time, data acquisition is not possible.
1. Open the Commands dialog box for the detector.
2. Select ValidateRaman to start the verification.
The following message appears in Chromeleon:
Make sure that
- the system is equilibrated and conditions are stable
- fluorescence-grade water is flowing through the cell
- the water is degassed (no air bubbles) and free of particles
Confirm with OK if you are sure that the above conditions are met.
The result (passed/failed) is displayed in the Chromeleon Audit Trail.
Passed: The calibration accuracy is within the allowed deviation range. The exact
wavelength can be found under RamanValidationWL. The ideal value for the
measurement is 397 nm.
Failed: The calibration of the detector is not sufficient. Perform a wavelength calibration
(→ page 91). If wavelength verification fails again after the recalibration, check the
causes and remedial actions outlined in section 6.3 (→ page 102).
Wavelength Calibration
You can perform wavelength calibration via Chromeleon. Wavelength calibration can take
a few minutes. During this time, data acquisition is not possible.
1. Open the Commands dialog box for the detector.
2. Select CalibrateRaman to start the calibration. The following message appears in
Chromeleon:
Make sure that
- the system is equilibrated and conditions are stable
- fluorescence-grade water is flowing through the cell
- the water is degassed (no air bubbles) and free of particles
Confirm with OK if you are sure that the above conditions are met.
3. The new calibration values are stored in the detector.
4. Perform wavelength verification.
Operating Instructions
Fluorescence Detectors FLD-3100 and FLD-3400RS
UltiMate 3000 Series:
Page 91