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6.10 Optimizing the Performance of the Device
6.10.1 General Guidelines
Charged Aerosol Detectors (VH-D20, VF-D20)
Operating Manual
This section provides information for best performance of the device
and gives hints on what you can do to optimize the performance further.
To optimize the performance of the device, consider the following
general guidelines:
•
Monitor the usage of specific device components that are subject to
wear and stress and schedule appropriate maintenance intervals
(see
Predictive Performance
•
Consider the influences of the parameters on the detection. See
Overview of Optimization Parameters
•
Ensure that the operating conditions are suitable. This includes:
Stable environmental conditions, such as a stable temperature
¨
No air drafts
¨
No vibrations or mechanical shocks caused by external sources
¨
Stable backpressure and correct waste line setup
¨
•
Degas the solvent in the Vanquish pump.
•
For guidelines and recommendations on the use of mobile phases
with the charged aerosol detector, see
the Detector
(} page 96).
•
Maintain the nitrogen or air source for your detector at regular
intervals to ensure optimum performance. Observe the instructions
in the User Documentation for the nitrogen generator.
•
Use a dedicated column for each specific analysis to avoid cross-
contamination of the column.
•
Do not use a column that has been used with non-volatile mobile
phases.
•
Columns that are unstable and/or have a tendency to release
particles from the column bed (i.e. "column bleed") will produce
noise in the detector. Avoid such columns.
•
When the mobile phase was stored over a period of time, high
background current may be observed. Replace old mobile phase
with freshly prepared mobile phase.
•
If you use the detector for the analysis of complex biological
samples, proper sample preparation is especially important.
•
If you perform a gradient, it is recommended to perform blank
injections with the desired method gradient profile prior to analysis.
This "pre-conditioning" of the column improves the performance of
both, the analytical column and the detector.
(} page 127)).
(} page 108).
Mobile Phase Guidelines for
6
•
Operation
Page 107
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