Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual page 6

UltiMate 3000 Series:
Fluorescence Detectors FLD-3100 and FLD-3400RS
4 Preparation for Operation (Startup) ........................................................................ 45
4.1 Overview .................................................................................................................. 45
4.2 General Precautions for Connecting Capillaries ...................................................... 46
4.3 Connecting the Drain System .................................................................................. 47
4.4 Installing the Flow Cell ............................................................................................ 48
4.5 Equilibrating the System .......................................................................................... 51
4.6 General Guidelines for Detector Operation ............................................................. 52
4.6.1 Mobile Phases ................................................................................................. 52
4.6.2 Mobile Phase Delivery System ....................................................................... 53
4.6.3 Flow Cells ....................................................................................................... 53
5 Operation and Maintenance ...................................................................................... 55
5.1 Power-Up ................................................................................................................. 55
5.2 Detector Display ...................................................................................................... 55
5.3 Menus on the Detector Display ................................................................................ 57
5.3.1 Detector Menus ............................................................................................... 58
5.3.2 General Menu Layout and Structure ............................................................... 59
5.3.3 Main Menu ...................................................................................................... 61
5.3.4 Configuration Menu ........................................................................................ 62
5.3.5 Diagnostics Menu ........................................................................................... 63
5.3.6 Service Menu .................................................................................................. 63
5.4 Operation with Chromeleon ..................................................................................... 64
5.4.1 Connecting to Chromeleon ............................................................................. 64
5.4.2 Direct Control ................................................................................................. 65
5.4.3 Automated Control ......................................................................................... 67
5.4.4 SmartStartup and SmartShutdown .................................................................. 71
5.5 Operational Settings ................................................................................................. 72
5.5.1 Detecting Liquid Leaks in the Detector .......................................................... 72
5.5.2 Adjusting the screen brightness or contrast .................................................... 72
5.6 Operational Modes of the Detector .......................................................................... 73
5.6.1 Single-Channel Mode ..................................................................................... 73
5.6.2 Multi-Channel Mode (FLD-3400RS Only) .................................................... 73
5.6.3 Zero Order Mode ............................................................................................ 74
5.6.4 2D Scans ......................................................................................................... 74
5.6.5 3D Scans (only Chromeleon 7.1 or later) ....................................................... 76
5.7 Optimizing Detector Performance ........................................................................... 77
5.7.1 Determining the Optimum Excitation and Emission Wavelength.................. 78
5.7.2 Sensitivity (Detector Sensitivity) .................................................................... 81
5.7.3 Filter Wheel Setting (FLD-3400RS only) ...................................................... 85
5.7.4 Response Time and Data Collection Rate ...................................................... 86
5.7.5 Multi-Channel Performance ........................................................................... 87
5.7.6 Flow Cell Temperature ................................................................................... 88
5.7.7 Lamp Mode ..................................................................................................... 88
5.7.8 Baseline Behavior ........................................................................................... 89
5.8 Monitoring System Functions .................................................................................. 90
5.8.1 Wavelength Calibration and Verification ....................................................... 90
Page ii Operating Instructions