Baseline Behavior - Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual

You can select the Lamp Mode directly in the Program Wizard (→ page 68) if you have
selected a single wavelength channel.
Tip: You can change the lamp mode during a measurement, for example, from
LongLife mode (between the peaks) to Standard or HighPower mode (for the
interesting peaks). This extends the lamp lifetime, without any loss in
sensitivity in the important areas. For further information about the modes for
the Xenon lamp, refer to the Fluorescence Method Development Handbook for
the detector.

5.7.8 Baseline Behavior

If wavelengths are switched or the sensitivity, filter wheel, or PMT setting are changed
during a program, the chromatogram may show baseline jumps. You can define how the
baseline behaves in these cases. Select whether you want to automatically perform an
autozero (Zero), to append the baseline to the previous signal (Append), or to let the
baseline "jump" to the new value (Free).
To set the baseline behavior in Chromeleon
1. Open the Commands dialog box for the detector.
2. Click BaselineBehavior.
3. Select the preferred setting from the list.
4. Click Execute.
Tip: Using the AutoZero command, you can perform an autozero any time, that is,
the intensity measured at the time of the autozero is subtracted from any
intensities measured thereafter. Always select a time at which no sample flows
through the cell to perform an autozero. The values are stored and can be reset
using the ClearAutoZero command.
Operating Instructions
Fluorescence Detectors FLD-3100 and FLD-3400RS
UltiMate 3000 Series:
Page 89