Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual page 92

UltiMate 3000 Series:
Fluorescence Detectors FLD-3100 and FLD-3400RS
Run a sample and read out the MaxPMTSaturation values in Chromeleon and evaluate
the results:
Value
< 30%
30% - 80%
80% - 99%
≥ 100%
If you do not know the expected concentrations of the analytes, reduce the Sensitivity even at
smaller values of MaxPMTSaturation.
Tips: The maximum saturation shown with MaxPMTSaturation affects the signals
of both PMTs with the parameters of all channels.
You can completely turn off fluorescence detection by setting the sensitivity to
Off. This may be required, for example, if you want to record the incoming
signal from a UV detector for a sample of high concentration, or to suppress an
extremely high peak that is not of interest for you but triggers an automatic
adjustment of the sensitivity.
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What you should do...
Increase the Sensitivity. The following is a rough guideline:
< 30 %: by one stage
< 15 %: by two stages
Then repeat the optimization, starting with step 1.
The Sensitivity value is optimal.
The Sensitivity should be reduced by 1 step to avoid undesired
saturation when concentration varies.
The Sensitivity must be reduced by at least one step. In most cases,
the detector has reduced the PMT sensitivity by autoranging.
Check the Audit Trail for the smallest sensitivity value after the
autoranging and repeat the optimization with this value, starting
from step 1.
Operating Instructions