Flow Cells - Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual

UltiMate 3000 Series:
Fluorescence Detectors FLD-3100 and FLD-3400RS

2.8 Flow Cells

The detector is shipped without a flow cell. The following flow cells are available for the
detector:
Flow cell type
Analytical,
pressure limit: 20 bar
Micro,
pressure limit: 40 bar
All parts of the flow cell that are exposed to solvents are made of fused silica, stainless
steel, or PTFE (Polytetrafluorethylene).
All flow cells are optimized for fast separations with no loss in chromatographic
resolution. An identification chip is fitted to the flow cell in the factory. The chip stores
unique information about the flow cell, including the cell type and serial number. When the
flow cell is installed, a contact on the flow cell connects the chip to the detector
electronics.
The flow cells are equipped with a temperature control. Flow cell and heat exchanger can be
heated to a user-defined temperature (→ page 88). The heat exchanger helps to adapt the
temperature of the mobile phase to the flow cell temperature before the mobile phase enters
the optical part of the cell. Note that the volume of the heat exchanger and inlet capillary
indicated in the table influences the retention times and peak widths.
The micro flow cell is optimized for best resolution in UHPLC, and is recommended for use
with 2.1 mm ID columns. The analytical flow cell provides higher sensitivity and a better
signal-to-noise ratio with most other columns.
To ensure optimum performance of the flow cells, observe the guidelines (→ page 53).
Page 24
Flow cell
Flow cell volume
material
Fused silica, 8 µL,
stainless steel,
Ø inlet capillary
PTFE
0.18 mm
Fused silica, 2 µL,
stainless steel,
Ø inlet capillary
PTFE
0.13 mm
Volume
Heat exchanger
and/or inlet capillary
6.3 µL
3.3 µL
Operating Instructions
Part no.
6078.4230
6078.4330