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1
Spectrophotometer Basics
Z-dimensions
Other Guidelines
Z-dimensions
6
Orion AquaMate 7000 Vis and Orion AquaMate 8000 UV-Vis
Note The pathlength of test tubes is not as well defined as that of square cuvettes.
Position cuvettes and test tubes so that the clear sides face the light beam, one clear side facing
the front of the instrument and the other facing the back.
Note Always place test tubes in the instrument in exactly the same orientation in the light
beam. An alignment mark on the test tube helps you orient the test tubes consistently and
correctly.
When using small aperture (small volume) cells:
• Always used cells with black masking
• Use the same cell (or cuvette) for your blank and your samples
The figure below illustrates the position of the light beam in the instrument.
Beam size specifications are shown below.
• Distance from bottom of cuvette to center of beam (Z-dimension): 8.5 mm
• Beam dimensions: 2 mm (wide) by 7 mm (tall)
Thermo Scientific
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