Kinetics; Chapter 21 Kinetics - Thermo Scientific Orion AquaMate 8000 UV-Vis User Manual

Kinetics

Thermo Scientific
The Kinetics application lets you measure the change in the sample absorbance as a function
of time. The local control software allows the determination of a linear rate over a particular
region, which can be defined after the data acquisition. Frequently used in enzymatic kinetics,
a factor can be multiplied by the slope of the linear rate fit to determine activity.
Computer software control lets you greatly expand the kinetics capabilities of your
instrument:
• Multicell parallel kinetics lets you monitor up to five reactions simultaneously. Extended
kinetics data acquisition exceeds the 400 data point limit of the embedded software.
• Software control streamlines the use of more sophisticated computer applications to
analyze kinetics data after collection.
Use Kinetics for:
• Recalling a Test
• Setting Up Test Parameters
• Cell Correction
• Measuring a Blank
• Measuring Samples
• Recalling and Recalculating Graphical Kinetics Results
• Rescaling and Recalculating Tabular Kinetics Results
• Modifying the scale of the plot
You can work with graphical or tabular data and perform the same functions with either.
However, the location of the function keys depends on the display type.
Note The Kinetics application lets you measure only one sample at a time.
Note The Kinetics application lets you collect up to 400 data points per run. When
setting test parameters, select the interval time and total run time accordingly.
To get started, press the Test button, highlight Kinetics and press Enter.
Orion AquaMate 7000 Vis and Orion AquaMate 8000 UV-Vis
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