Chapter 25 Calculations For Software - Thermo Scientific Orion AquaMate 8000 UV-Vis User Manual

Calculations for Software
Calculation Calculation(s)
Standard Curves
Partial sums
SX
SY
SXX
SYY
SXY
SQX
SQY
SSXY
Where:
x
1
y
1
N = number of standards
Linear regression A = A(c)
(general case)
Where:
A = absorbance
c = concentration
A(c) is defined by an equation of the form:
A(c) = a
Where:
a
0
a
1
(The coefficients are computed using the least squares
method.)
Thermo Scientific
 
x
i
 
y
i
  2
x
i
  2
y
i
 
x y
 
i i
2
   
x x N *
 
i
2
   
y y N *
  
i
2
   
x x y y
i i
th
= Concentration of i standard
th
= Absorbance of i standard
4 3 2
c +a c +a c +a c+a
4 3 2 1
= Y-axis intercept
...a = coefficients
4

2
SXX SX
 2
SYY SY
 
N * SXY SX * SY
0
Orion AquaMate 7000 Vis and Orion AquaMate 8000 UV-Vis
Graphs
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