Optical dissolved oxygen measurement system (65 pages)
Summary of Contents for Thermo Scientific Orion AquaMate UV-Vis
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Thermo Scientific Orion AquaMate UV-Vis and Vis Spectrophotometers 269-289800 • Revision C • July 2015...
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Table of Contents Chapter 1 ........................8 Spectrophotometer Introduction ................. 8 Spectrophotometer Overview ...................... 8 Packing List ..........................9 Orion AquaMate User Documentation CD ............... 10 Intended Use ..........................10 Safety and Special Notices ......................11 Chapter 2 ........................12 Spectrophotometer Basics ..................
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Chapter 5 ........................46 Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate ....46 Orion AQUAfast Colorimetric Reagents Compatible with Orion AquaMate Instruments ... 46 Orion AQUAfast Reagent Instructions ..................48 Recommendations for Avoiding Measurement Errors ............48 AC2002 Alkalinity-M (Alkalinity to pH 4.3) Tablet Test ............. 49 AC3002P Alkalinity-P (Alkalinity to pH 8.2) Tablet Test ...........
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ACD095 Phosphate (Total) Digestion Tube Test ............104 ACD095AH Phosphate (Acid Hydrolysable) Digestion Tube Test ......... 106 AC2060 Silica Tablet Test ....................108 AC2061 Silica with Phosphate Removal Tablet Test ............. 109 AC4P60 Silica Powder Pack Test .................. 110 AC4P82 Sulfate Powder Pack Test ................111 AC2016 Sulfide Tablet Test ...................
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Chapter 10 ......................... 178 Maintenance ......................178 Routine Care ..........................179 Cleaning and Maintaining Vials and Cuvettes ..............179 Cleaning the Windows of the Sample Compartment ............181 Changing the Fuse ........................182 Replacing the Tungsten Lamp ....................184 Chapter 11 ......................... 186 Customer Services ....................
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Spectrophotometer Introduction C H A P T E R 1 Spectrophotometer Overview Thermo Scientific Orion AquaMate UV-Vis and Vis spectrophotometers offer the following ™ ™ ™ features and benefits: Easy operation using the 260+ preprogrammed methods for common colorimetric reagents •...
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110V US/Japan power cord, Catalog Number AQ110V • 220V Euro power cord, Catalog Number AQ220V • 240 V UK/Singapore power cord, Catalog Number AQ240V • Note: The China/Australia/New Zealand power cord, Catalog Number AQCHNV, must be ordered separately. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Orion Laboratory Product Warranty Card • USB Driver • Intended Use Please read this user manual thoroughly. Any use outside of these instructions may invalidate the instrument warranty and cause permanent damage to the instrument. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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For Research Use Only. This instrument is not a medical device and is not intended to be used for the prevention, diagnosis, treatment or cure of disease. Warning: Avoid an explosion or fire hazard. This instrument is not designed for use in an explosive atmosphere. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Spectrophotometer Basics C H A P T E R 2 Spectrophotometer Components The following are some of the major components visible on the outside of the instrument: Sample Compartment Keypad USB Port Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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On/Off Switch Warning: Avoid shock hazard. Always turn off the instrument and unplug it from the wall outlet or power strip before you unplug the power cord from the instrument connector. Instrument Keypad Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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B is the blank position and 1-5 are the sample positions when using the Auto 6 sample positioner setting and B is the blank position and 2 and 4 are the sample positions when using the Auto 3 sample positioner setting. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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B location on the sample positioner and measure five samples in the 1-5 locations on the sample positioner. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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The figure below illustrates the position of the light beam in the instrument. Beam size specifications are shown below. Distance from bottom of the vial/cuvette to center of beam (Z-dimension): 8.5 mm • Beam dimensions: 2 mm (wide) by 7 mm (tall) • Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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4. With the other hand, tighten the captive thumbscrew or thumbscrews. 5. Close the sample compartment door. Note: If the carousel is not aligned correctly, you will not be able to tighten the thumbscrews. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Note: When the carousel is installed and Manual 6 is selected, the instrument always considers the vial in the B position as a blank. This means that even after measuring the blank the first time, samples can only be placed in positions 1 through 5. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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““Fatal Error: 8 Press Esc to return to main menu.” If this message is displayed, press the esc key and then press the Run Test function key to re-initialize the vial holder carousel. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Highlight the desired parameter (i.e. Wavelength) and start typing the numeric value. The Entry window will open with the range of the parameter shown below. Type the desired value and then press the enter key. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Name window will open. Press the or key to highlight a character, press the Add Character function key and repeat until the desired name is shown. Press the Accept Name function key to save the entered name. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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3. If interfacing the instrument with an external printer, press the ▲or ▼ key to highlight the External option and then press the enter key to set the External option to On. 4. Press the esc key to return to the main Utility menu. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Approve any security messages by clicking the Install button. 12. Once the installation of the USB driver software is complete, click the Close button. 13. Record the COM port number of the USB connection in the Device Manager window. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Press the Set Minute function key, use the numeric keypad to enter the minute value and press the enter key. c. When using the am/pm time format, press the Set AM/PM function key until the correct setting is shown. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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To set the baseline expiration time, press the ▲or ▼ key to highlight the Baseline Expiration (hr:min) option, press the enter key, use the numeric keypad to enter the time value and press the enter key to save the setting. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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2. Press the + function key, - function key, x function key or ÷ function key. 3. Use the numeric keypad to enter the second value. 4. Press the enter key to perform the calculation. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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When saving a test, it can be saved to an external memory device using the front USB port or saved to the instrument’s internal memory. 1. After setting the test parameters, highlight Test Name and press the enter key. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Press the or key to highlight Include as a SmartStart test or Do not include as a SmartStart test and press the enter key. 5. The test name will be shown with [Saved] after the name. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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5. Press the ▲or ▼ key to highlight the desired test and press the Load Test function key. 6. The test will be loaded in the test technique menu. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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If you select more than one test as SmartStart tests, when the instrument is powered on it • will automatically display a menu containing only those tests. Note: The default menu can always be accessed by pressing the test key. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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6. Press the test key to access the Test Types menu. 7. Press the Smart Start function key to open the Smart Start menu. 8. Press the ▲or ▼ key to highlight a test and press the enter key to start the test. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Water Analysis Test Menu C H A P T E R 4 Preprogrammed Methods Thermo Scientific Orion AquaMate 8000 UV-Vis and AquaMate 7000 Vis spectrophotometers include a USB memory stick that contains over 260 preprogrammed methods for use with Thermo Scientific...
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Note: A copy of the methods on the USB memory stick should be saved to a computer or other data source in case the USB memory stick is accidentally deleted or otherwise corrupted. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Press the test key to access the Test Types menu. 5. Press the ▲or ▼ key to highlight Water Analysis and press the enter key. 6. Press the Stored Tests function key. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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8. Press the ▲or ▼ key to highlight the test method and press the Load Test function key. 9. Press the Save Test function key. 10. Press the ▲or ▼ key to highlight Save as an internal test and press the enter key. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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12. Press the ▲or ▼ key to highlight Include as a SmartStart test or Do not include as a SmartStart test and press the enter key. 13. Press the Run Test function key to begin using the test method. Refer to the specific reagent chemistry instructions for detailed procedures. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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1. Press the test key to access the Test Types menu. 2. Press the SmartStart function key. 3. Press the ▲or ▼ key to highlight the test method and press the Load Test function key. 4. Press the Run Test function key. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Note: Ideally, the same vial should be used or one that has been matched to the sample vial. 5. Close the lid and press the Measure Blank function key. The instrument will set the measurement wavelength and perform a blank measurement. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Using the Reverse Color Feature section for detailed instructions. Note: If the Statistics option is set to Off in the test parameters menu, no Average or Std Dev information will be displayed in the measurement screen. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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5. Enter the concentration of the standard using the numeric keypad and press the enter key. 6. Wipe the exterior of the blank vial and place the blank vial into the holder in the sample chamber and close the chamber door. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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16. Press the Run Test function key. When running a test with an adjustment correction factor in use, the display will show ( 1pt Adj ) next to the test name. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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4. Place the vial into the holder in the sample chamber and close the chamber door. 5. Press the Measure Blank function key to measure the blank. 6. Open the sample chamber door and remove the vial from the holder. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Close the vial tightly with the cap and mix the contents. Wipe the exterior of the vial. 12. Place the vial into the holder in the sample chamber and close the chamber door. 13. Press the Measure Sample function key to display the results. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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(mg/L) = 2301 (abs) - 3 The equation is: Result = -3 + 2301*(Abs) Set the factors as: A = -3 B = 2301 C = 0 D = 0 Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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5. Press the ▲or ▼ key to highlight the A, B, C or D factor, press the enter key, use the numeric keypad to enter the factor value and press the enter key. Repeat for each required equation factor. 6. Save or run the test using the new test parameters. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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- i.e. due to the formation of condensate in the area of the lens or on the vial. Specified tolerances at T = 20 °C. For the best results, use a pipette to measure and add samples to vials or beakers. • Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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9. Press the Measure Sample function key to display the result in mg/l total alkalinity. Notes: The terms total alkalinity, alkalinity-m, m-value and alkalinity to pH 4.3 are identical. • For accurate results, exactly 10 ml of water sample must be taken for the test. • Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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For accurate test results, exactly 10 ml of water sample must be taken for the test. • This method was developed from a volumetric procedure for the determination of • alkalinity-p. Due to undefined conditions, the deviations from the standardized method may be greater. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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To get accurate results the sample temperature must be between 20 °C and 25 °C. • A low test result may be given in the presence of fluorides and polyphosphates. The effect • of this is generally insignificant unless the water has fluoride added artificially. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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To get accurate results the sample temperature must be between 20 °C and 25 °C. • A low test result may be given in the presence of fluorides and polyphosphates. The effect • of this is generally insignificant unless the water has fluoride added artificially. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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The temperature of the sample is important for full color development. At a temperature • below 20 °C, the reaction period is 15 minutes. Conversion: mg/l NH = mg/l N x 1.29 • mg/l NH = mg/l N x 1.22 Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Less common interferences such as hydrazine and glycine will cause intensified Hydrazine, Color, colors in the prepared sample. Turbidity and color will give erroneous high values. Turbidity Samples with severe interferences require distillation. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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To produce the blank add an iron standard solution with the same iron concentration to the vial instead of deionized water. Conversion: mg/l NH = mg/l N x 1.29 • mg/l NH = mg/l N x 1.22 Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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To produce the blank, add an iron standard solution with the same iron concentration to the vial instead of deionized water. Conversion: mg/l NH = mg/l N x 1.29 • mg/l NH = mg/l N x 1.22 Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Strong alkaline or acidic water samples must be adjusted between pH 6 and pH 7 before the reagent is added (use 0.5 mol/l sulfuric acid or 1 mol/l sodium hydroxide). Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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10 ml of the diluted sample should be mixed with the reagent and the measurement repeated. Oxidizing agents such as chlorine or ozone interfere as they react in the same way as • bromine. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Ions that also form deposits with silver nitrate in acidic media, such as bromides, iodides • and thiocyanates, interfere with the analysis. Highly alkaline water should, if necessary, be neutralized using nitric acid before analysis. • Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Vial cleaning: As many household cleaners (i.e. dishwasher detergent) contain reducing • substances, the subsequent determination of chlorine may show lower results. To avoid any measurement errors, only use glassware free of chlorine demand. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Oxidizing agents such as bromine or ozone interfere as they react in the same way as • chlorine. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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The DPD color development is carried out at a pH value of 6.2 to 6.5. The reagents • therefore contain a buffer for the pH adjustment. Strong alkaline or acidic water samples Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Oxidizing agents such as bromine or ozone interfere as they react in the same way as • chlorine. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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(EN ISO 7393-2, 5.3). Preparing the sample: When preparing the sample, the escape of chlorine gases, i.e. by • pipetting or shaking, must be avoided. The analysis must take place immediately after taking the sample. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Oxidizing agents such as bromine or ozone interfere as they react in the same way as • chlorine. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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10 ml of the diluted sample should be mixed with the reagent and the measurement repeated. Oxidizing agents such as bromine or ozone interfere as they react in the same way as • chlorine. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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10 ml of the diluted sample should be mixed with the reagent and the measurement repeated. Oxidizing agents such as bromine or ozone interfere as they react in the same way as • chlorine. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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9. Place the vial into the holder in the sample chamber and close the chamber door. 10. Press the Measure Sample function key to display the result in mg/l chlorine. Notes: Oxidizing agents interfere as they react in the same way as chlorine. • Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Tablet straight from the foil into the vial. Crush the tablet with a clean stir rod. Close the vial tightly with the cap and swirl or invert several times until the tablet is dissolved. 9. Transfer the contents of the second vial into the first vial. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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10 ml of the diluted sample should be mixed with the reagent and the measurement repeated. Oxidizing agents such as chlorine or ozone interfere as they react in the same way as • chlorine dioxide. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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(highest absorbance). The color of samples prepared with the reagent will decrease as the concentration increases for this method. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Samples can be measured when the chloride content does not exceed 1000 mg/l. • In exceptional cases, compounds contained in the water cannot be oxidized adequately, so • results may be lower than reference methods. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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In exceptional cases, compounds contained in the water cannot be oxidized adequately, so • results may be lower than reference methods. For samples under 100 mg/l it is recommended to repeat the test using the COD low range • test (CODL00). Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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For samples under 1000 mg/l it is recommended to repeat the test using the COD mid • range test (CODH00) or for samples under 100 mg/l it is recommended to repeat the test using the COD low range test (CODL00). Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Alternatively, the AC202950 method can be used with 50mm rectangular vials. All blank • and sample volumes must remain the same as those specified in these instructions, so samples may need to be prepared in separate containers and then transferred into the selected vial. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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If turbidity remains and turns black, silver interference is likely. Add 10 drops of saturated potassium chloride solution to 75 ml of water sample. Filtrate through a fine filter. Use 10 ml of the filtered water sample to perform test. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Exceeding the measurement range: samples with concentration above 90 mg/l must be • diluted with water free of cyanuric acid. 10 ml of the diluted sample should be tested as described above and the displayed results calculated using the dilution factor. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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The one point calibration process needs to be performed for each new batch of SPADNS reagent solution (see Standard Methods 20 ed., 1998, APHA, AWWA, WEF 4500 F D, 4.a). Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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1:1 dilution of the sample prior to use and subsequent multiplication of the result by 2. SPADNS reagent solution contains arsenite. Chlorine concentrations up to 5 mg/l do not • interfere. Seawater and wastewater samples must be distilled. • Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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10. Press the Measure Sample function key to display the result in mg/l total hardness. Notes: Strong alkaline or acidic water samples must be adjusted between pH 4 and pH 10 before • the tablet is added (use 1 mol/l hydrochloric acid or 1 mol/l sodium hydroxide). Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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If the result is above the detection limit of 0.05 mg/l, you should only use the reagent with reservations, as there may be a major deviation in results. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Therefore add 1 ml concentrated sulfuric acid and 1 ml concentrated nitric acid to 100 ml water sample and boil to approximately half volume. After cooling down, proceed as described above. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Very strong alkaline or acidic samples must be adjusted to a pH value between 3 and 5 • before analysis. Accuracy is not affected by undissolved powder. • Water samples containing visible rust should be allowed to react for at least 5 minutes. • Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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9. Wait for a reaction period of 5 minutes. 10. Place the vial into the holder in the sample chamber and close the chamber door. 11. Press the Measure Sample function key to display the result in mg/l manganese. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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The turbidity should disappear after the PAN indicator solution is added. Water samples containing more than 5 mg/l iron should be allowed to react for at least 10 • minutes. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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5, as manganese may precipitate. Interferences: • Interfering Substance Interference Level Calcium Greater than 700 mg/l Chloride Greater than 70,000 mg/l Iron Greater than 5 mg/l Magnesium Greater than 100,000 mg/l Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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– so it is very important to perform the test procedure as described. Substances which may interfere when present in concentrations at: • Aluminum 50 mg/l Chromium 1000 mg/l Iron 50 mg/l Nickel 50 mg/l Nitrite All Levels Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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10. Place the sample vial into the holder in the sample chamber and close the chamber door. 11. Press the Measure Sample function key to display the result in mg/l nitrate as N. Notes: Some solids may not dissolve. • Conversion: mg/l NO = mg/l N x 4.43 • Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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It is unlikely in practice that these interfering ions will occur in such high concentrations that they cause significant reading errors. Conversion: mg/l NO = mg/l N x 3.29 • Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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In samples with very high concentrations of nitrate (> 100 mg/l N) a small amount of nitrite will be found. Such high levels of nitrate appear to undergo a slight amount of reduction to nitrite, either spontaneously or during the reaction time of the test. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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16. Wait for a reaction period of 5 minutes. 17. Load and run the ACD004 method. 18. Place the blank vial into the holder in the sample chamber and close the chamber door. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Application: for water, wastewater and seawater. • Interfering substances that resulted in a concentration change of 10%: Bromide more than • 60 mg/l and chloride more than 1000 mg/l produce positive interferences. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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16. Wait for a reaction period of 5 minutes. 17. Load and run the ACD007 method. 18. Place the blank vial into the holder in the sample chamber and close the chamber door. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Application: for water, wastewater and seawater. • Interfering substances that resulted in a concentration change of 10%: Bromide more than • 60 mg/l and chloride more than 1000 mg/l produce positive interferences. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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8. Fill a second clean AQUAfast 24mm round vial with 10 ml of sample. Add one Glycine Tablet straight from the foil to the vial. Crush the tablet with a clean stir rod. Close the vial tightly with the cap and swirl several times until the tablet is dissolved. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Oxidizing agents such as bromine and chlorine interfere as they react in the same way as • ozone. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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– 0.21 – 0.26 – 0.29 The values of Parson and Douglas (1926) are based on the use of Clark and Lubs buffers. • 1 Mol NaCl = 58.4 g/l = 5.8 % Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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This can be minimized by using a pipette (0.18 ml phenol red solution is equivalent to 6 drops). After use, replace the bottle cap securely. • Store the phenol red solution in a cool, dry place ideally at between 6 °C and 10 °C. • Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Organically combined phosphates are converted to ortho-phosphate ions by heating with acid and persulfate. The amount of organically combined phosphates can be calculated: mg/l phosphate (organic) = mg/l phosphate (total) - mg/l phosphate (acid hydrolysable) Phosphate, ortho = Phosphorus, reactive • Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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(organic) = mg/l phosphate (total) - mg/l phosphate (acid hydrolysable) The ortho-phosphate ions react with the vanadate-molybdate reagent under acid conditions • to form a yellow colored product. Phosphate, ortho = Phosphorus, reactive • Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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50 mg/l Chromium greater than 100 mg/l Silicate greater than 10 mg/l Copper greater than 10 mg/l Sulfide at any level Iron greater than 100 mg/l Zinc greater than 80 mg/l Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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50 mg/l Chromium greater than 100 mg/l Silicate greater than 10 mg/l Copper greater than 10 mg/l Sulfide at any level Iron greater than 100 mg/l Zinc greater than 80 mg/l Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Pretreatment of the sample with acid and heat provides the conditions for hydrolysis of the condensed inorganic forms. Organically combined phosphates are converted to ortho-phosphate ions by heating with Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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50 mg/l Silicate greater than 10 mg/l Sulfide at any level Zinc greater than 80 mg/l Conversions: mg/l PO = mg/l P x 3.07 • mg/l P = mg/l P x 2.29 Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Pretreatment of the sample with acid and heat provides the conditions for hydrolysis of the condensed inorganic forms. Organically combined phosphates are converted to ortho-phosphate ions by heating with Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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50 mg/l Silicate greater than 10 mg/l Sulfide at any level Zinc greater than 80 mg/l Conversions: mg/l PO = mg/l P x 3.07 • mg/l P = mg/l P x 2.29 Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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12. Place the vial into the holder in the sample chamber and close the chamber door. 13. Press the Measure Sample function key to display the result in mg/l silica. Notes: The tablets must be added in the correct sequence. • Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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The tablets must be added in the correct sequence. • Phosphate ions do not interfere under the given reaction conditions. • If phosphate is known to be absent, the addition of the Silica PR Tablet may be omitted. • Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Does not interfere at concentrations less than 50 mg/l PO At 60 mg/l PO the interference is approximately 2% At 75 mg/l PO the interference is approximately 11 % Sulfide Interferes at all levels Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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9. Place the vial into the holder in the sample chamber and close the chamber door. 10. Press the Measure Sample function key to display the result in mg/l sulfate. Notes: If sulfate ions are present a cloudy solution will appear. • Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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To avoid loss of sulfide collect the sample carefully with a minimum of aeration. It is • essential to test the sample immediately after collection. The sample temperature should be 20 °C. A different temperature can lead to higher or • lower results. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Measuring the reagent blank needs to be done with each sample analysis. • The tablets must be added in the correct sequence. • In the case of high levels of residual chlorine, perform the analysis with a dechlorinated • water sample. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Hazen units depending on the specific procedures. In the United States, the National Secondary Drinking Water Regulation for color is a maximum of 15 color units. The World Health Organization recommends that drinking water color does not to exceed 15 true color units. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Spectrophotometers allow for more accurate and precise results than colorimeters, but are • more expensive. Lowest detection limits and better accuracy at low levels can be achieved on a spectrophotometer by choosing a sample cell with a longer cell path length. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Thermo Scientific Orion AquaMate Spectrophotometers for Color Measurement The Thermo Scientific Orion AquaMate AQ8000 UV-Vis and AQ7000 Vis spectrophotometers have the required wavelengths to analyze for any color measurement – by preprogrammed methods, by a user-generated standard curve, or by multiple wavelengths, if desired. The following table describes some of the color methods which may be tested on the Orion AQ7000 and AQ8000 spectrophotometers.
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Application Notes Application notes for color testing are available through your local technical sales representative, our technical support service, our website and other Thermo Scientific web locations. These notes give detailed information on how to test for color on our Thermo Scientific Orion AquaMate spectrophotometers: AQ7000 visible range and AQ8000 UV/Vis range.
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2. Standard Methods 5910B, UV-Absorbing Organic Constituents. www.standardmethods.org 3. Orion AquaMate 8000 UV-Vis Instrument User Guide Recommended Equipment Thermo Scientific Orion AquaMate AQ8000 spectrophotometer • Filtration apparatus (e.g., Fisher XX1004707); 0.45-µm filters, 47 mm (e.g., Fisher • HNWP04700) Vacuum source-aspirator, air flow or water flow, hand-operated or low pressure electric •...
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For UVA, UV254: Set up the filtration apparatus with a 0.45 um filter. Wash the filter with 50 mL DI and discard the rinse water. Filter 50 mL of the sample. Test the filtrate. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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The average AQ8000 organic carbon concentration result (calculated per SM 5910B) is • within 0.1 mg/L of the expected value; 0.4% difference (99.6% recovery) from the expected value of 25.0 mg/L organic carbon. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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1. If the preprogrammed method is not on the memory stick, download the method file from the our Online Library at www.thermoscientific.com/waterlibrary, or call Technical Support. 2. Alternately, use disposable cuvettes formulated for UV measurements, available in the 1 cm (10 mm) cell path. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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To edit a standard curve, including changing the number of standards, selecting a different • curve fit or deleting points from the curve To measure samples using the standard curve and to view and save measurement data • To recall existing standard curve methods • Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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See the Parameters section for more information on the test parameter settings. b. See the Sample Positioner Setting for the Vial Holder Carousel section for detailed information on selecting the appropriate Sample Positioner setting. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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4. If Manual 6 or Single Cell Holder is selected for the Sample Positioner setting: a. For each standard, use the numeric keypad to enter the concentration of the standard and press the enter key to save the value. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Standard function key and press the enter key to measure the standard. Repeat for each standard. 5. When all of the standards have been measured, the absorbance of each standard will be displayed with the slope, intercept and correlation coefficient of the standard curve. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Place the blank and samples in the appropriate positions on the vial holder carousel and press the Measure Samples function. b. Confirm that the blank and samples are in the appropriate positions and press the enter key to begin taking measurements. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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USB memory stick. Do not use more than eight characters when naming the saved data file. The data file will be saved as a .TXT file in the Thermo folder on the USB memory stick. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Chapter 6 Standard Curve Test Menu Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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If Clear Measurements is selected, all absorbance measurements will be removed from the standard curve data. e. If Reset Standards is selected, all concentration and absorbance measurements will be removed from the standard curve data. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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2. Press the ▲or ▼ key to highlight the desired curve fit and press the enter key. 3. The instrument will apply the selected curve fit to the data and display the new fit. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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4. Press the ▲or ▼ key to highlight Load internal test or Load test from USB drive and press the enter key. 5. Press the ▲or ▼ key to highlight the desired test and press the enter key. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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To view and manipulate scanning data, including rescaling graphical scan data, • determining peak height using a 3-point net equation, calculating the area under a curve and labeling peaks and valleys To recall existing scanning methods • Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Scan tests only measure one sample at a time, so Auto 6 and Auto 3 Sample Positioner settings are not fully functional. 4. Press the Save Test function key and follow the onscreen prompts to save the test. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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6. Press the Measure Sample function key. 7. When the scan is complete, a graph of the scanned measurement will be displayed. Press the Tabular/Graph function key to switch between tabular and graphical displays. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Chapter 7 Wavelength Scanning Test Menu Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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1. In the graphical display, press the Edit Graph function key. 2. Press the Edit Scale function key to modify the scale. 3. In the Edit Scale menu: Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Modify the scan data graph by performing calculations on the data. 1. In the graphical display, press the Edit Graph function key. 2. Press the Math function key to modify the graph by performing calculations on the data. 3. In the Math menu: Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Press the ←Cursor and Cursor→ function keys to position the left cursor line at the desired wavelength value. Press the Next Cursor function key. iii. Press the ←Cursor and Cursor→ function keys to position the right cursor line at the desired wavelength value. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Press the ▲or ▼ key to highlight the type of labels to display and press the enter key. The instrument will label up to nine peaks or valleys on the scanned data graph. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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End nm function key. 4. The instrument will highlight the selected data points. To display the graph using the highlighted data points, press the Graph function key. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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4. Press the ▲or ▼ key to highlight Load internal test or Load test from USB drive and press the enter key. 5. Press the ▲or ▼ key to highlight the desired test and press the enter key. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Absorbance (A), % Transmittance (%T), or Concentration, the measurement results appear, along with the type of measurement, date and time, wavelength and vial position. In the main measurement mode, press the Change Mode function key to toggle between Absorbance, %Transmittance and Concentration. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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2. Place the sample in the appropriate position on the vial holder carousel. If needed, use the Sample Positioner keypad to set the vial location. 3. The absorbance (ABS) or percent transmittance (%T) measurement will be shown on the instrument display. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Press the ▲or ▼ key to highlight More parameters… and press the enter key to view additional parameters displayed on the second page. 4. Press the Save Test function key and follow the onscreen prompts to save the test. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Sample function key. Repeat for each sample. 5. When all of the samples have been measured, press the Save Data function key and follow the onscreen prompts to save the sample measurements to the USB memory stick. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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4. Press the ▲or ▼ key to highlight Load internal test or Load test from USB drive and press the enter key. 5. Press the ▲or ▼ key to highlight the desired test and press the enter key. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Absorbance, % Transmittance and Concentration. When the mode is set to Conc/Std or Conc/Factor, these capabilities are provided: Measuring a Blank • Measuring a Standard or Entering a Factor • Setting the Wavelength • Measuring Samples • Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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7. Place the sample in the appropriate position on the vial holder carousel. If needed, use the Sample Positioner keypad to set the vial location. The concentration measurement will be shown on the instrument display. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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6. Place the sample in the appropriate position on the vial holder carousel. If needed, use the Sample Positioner keypad to set the vial location. The concentration measurement will be shown on the instrument display. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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▲or ▼ key to highlight Conc/Std or Conc/Factor and press the enter key. 4. Highlight and change the displayed test parameters, including Test Name, Wavelength, Concentration of Standard, Factor, Units and Sample Positioner. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Place the blank and standard in the appropriate positions on the carousel. b. Press the Measure Standard function key. Confirm that the blank and standard are in the appropriate positions on the carousel and press the enter key. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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4. If Manual 6 or Single Cell Holder is selected for the Sample Positioner setting: a. Place the blank in the B position on the vial holder carousel and press the Measure Blank function key. Once the blank has been measured, remove it from the carousel. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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4. Press the ▲or ▼ key to highlight Load internal test or Load test from USB drive and press the enter key. 5. Press the ▲or ▼ key to highlight the desired test and press the enter key. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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4. Press the Set nms function key to add the wavelengths and factors. Factors can only be added when the measurement mode is set to Conc/Factor. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Sample function key. Repeat for each sample. 5. When all of the samples have been measured, press the Save Data function key and follow the onscreen prompts to save the sample measurements to the USB memory stick. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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4. Press the ▲or ▼ key to highlight Load internal test or Load test from USB drive and press the enter key. 5. Press the ▲or ▼ key to highlight the desired test and press the enter key. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Wavelength 2 and Sample Positioner. See the Parameters section for more information on the test parameter settings. 4. Press the Save Test function key and follow the onscreen prompts to save the test. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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4. Press the ▲or ▼ key to highlight Load internal test or Load test from USB drive and press the enter key. 5. Press the ▲or ▼ key to highlight the desired test and press the enter key. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Wavelength 2, Factor 1, Factor 2, Units and Sample Positioner. See the Parameters section for more information on the test parameter settings. 4. Press the Save Test function key and follow the onscreen prompts to save the test. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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4. Press the ▲or ▼ key to highlight Load internal test or Load test from USB drive and press the enter key. 5. Press the ▲or ▼ key to highlight the desired test and press the enter key. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Wavelength 2, Wavelength 3, Factor, Units and Sample Positioner. See the Parameters section for more information on the test parameter settings. 4. Press the Save Test function key and follow the onscreen prompts to save the test. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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4. Press the ▲or ▼ key to highlight Load internal test or Load test from USB drive and press the enter key. 5. Press the ▲or ▼ key to highlight the desired test and press the enter key. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Setting the Parameters for Kinetics Method 1. Press the test key to access the Test Types menu. 2. Press the ▲or ▼ key to highlight Kinetics and press the enter key. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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4. Press the Save Test function key and follow the onscreen prompts to save the test. Taking Measurements with Kinetics Method Once the parameters have been set for the kinetics test: 1. Press the Run Test function key. 2. Prepare the blank and sample that will be measured. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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4. Press the ▲or ▼ key to highlight Load internal test or Load test from USB drive and press the enter key. 5. Press the ▲or ▼ key to highlight the desired test and press the enter key. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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3. When the cursor line is in the correct position, press the Set Min X or Set Max X function key to accept the selected point. 4. The instrument will redraw the plot using the selected minimum and maximum values and display the recalculated rate and result. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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1. With tabular kinetics data displayed on the Edit Data screen, press the ▲or ▼ key to highlight the appropriate data point in the table. 2. Press the Start Time or End Time function key to display the recalculated rate and result. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Performance Verification C H A P T E R 9 Performance Verification Tests Thermo Scientific Orion AquaMate 8000 UV-Vis and AquaMate 7000 Vis spectrophotometers offer performance verification tests that assist with checking the performance of the instrument: Wavelength Accuracy – Internal •...
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Make sure the lamp compartment is clear of obstructions. • Warning: Do not open the lamp compartment unless the instrument power is OFF. Warning: Do not turn the instrument power ON unless the lamp compartment is closed. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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5. Once the test is complete, the results will indicate pass or fail for each wavelength. If the test fails, follow these guidelines: Repeat the test twice to verify that the test is failing consistently. • Contact technical support for more troubleshooting advice. • Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Use the numeric keypad to enter the tolerance for the entered wavelength in the entry field and press the enter key. c. Repeat for each standard. 5. Verify that the wavelengths and tolerances are set correctly. 6. Press the Start Test function key. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Repeat the test twice to verify that the test is failing consistently. • Make sure the target and tolerance values you entered for the calibrated wavelength are • the same as the values on the calibration certificate for the standard. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Use the numeric keypad to enter the absorbance or %T value for the entered wavelength in the entry field and press the enter key. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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If the test fails, follow these guidelines: Repeat the test twice to verify that the test is failing consistently. • Follow the guidelines provided with the standard reference materials. • Contact technical support for more troubleshooting advice. • Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Repeat the test twice to verify that the test is failing consistently. • Make sure the instrument is warmed up for at least 30 minutes, with the standby mode • feature turned off. Contact technical support for more troubleshooting advice. • Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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2. Press the ▲or ▼ key to highlight Performance Verification and press the enter key. 3. Press the ▲or ▼ key to highlight Stray Light and press the enter key. 4. In the Stray Light screen, make sure the wavelengths and tolerances are set correctly. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Make sure all the filters being used are specifically designed to measure stray light at the • specific wavelengths. Verify that the filters are placed in the correct positions. • Contact technical support for more troubleshooting advice. • Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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To protect both yourself and the instrument, be sure to contact an authorized service representative to perform any service procedure you do not feel comfortable performing. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Dilute nitric acid (<10%) rinse Copious water rinse Dilute nitric acid (<10%) rinse Aqueous Salt solutions Copious water rinse Warm water with detergent Aqueous Basic solutions Dilute nitric acid (<10%) rinse Copious water rinse Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Use a neutral pH detergent (Triton X-100), if available, to dilute acid wash; water Detergent rinse to remove residue Note: Do not use 5M nitric acid on an anti-reflection mirror coated vials or cuvettes. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Use the liquid and a soft, lint-free cloth to clean the windows. Do not apply too much pressure or the surface of the windows may be damaged. Be sure to remove all fingerprints. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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2. Position the instrument so you can access the power entry module on the back of the instrument. 3. Remove the power cord. 4. Insert a flat-blade screwdriver into the notch on the fuse cover and pry off the cover. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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9. Replace the power cord. 10. Plug the instrument back in to the wall outlet or power strip and turn on the power. Note: If the fuse blows again, contact your distributor or technical support. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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3. Loosen the captive screw on the lamp door by rotating it counterclockwise about ¼ turn. Aligning the lamp assembly Captive screw on lamp door Thumbscrew Lamp assembly Tab on cable connector 4. Raise the lamp door. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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14. Turn on the instrument and press the utility key. 15. Press the arrow keys to highlight Lamp Hours and press the enter key. 16. Press the enter key to reset the lamp hours to zero. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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For the most current warranty information, refer to the Thermo Scientific Orion warranty card included on the Thermo Scientific Orion AquaMate literature CD and available online at www.thermoscientific.com/water.
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4. Press the ▲or ▼ key to highlight the PC option and press the enter key to set the PC option to On. 5. Press the esc key until the instrument returns to the main measurement screen. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Right-click the Computer button. c. Click the Properties button. d. Click the Device Manager button. 5. Double-click the Spectrophotometer button on the Device Manager window. 6. Click the Update Driver button on the Spectrophotometer Properties window. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Chapter 11 Customer Services 7. Click the Browse my computer for driver software button. 8. Click the Browse button. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Select the Vista64 folder when using a computer with 64-bit Microsoft 7 or Vista operating system. c. Select the XP32 folder when using a computer with Microsoft XP operating system. 10. Click the Next button. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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2. Open the data collection software program by clicking the Realterm icon on the computer desktop. 3. Click the Port tab on the RealTerm window. Confirm that the RealTerm communication settings match the appropriate AquaMate communcation settings. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Chapter 11 Customer Services 4. Click the Port dropdown menu and select the COM port number of the Spectrophotometer USB connection. 5. Click the Baud dropdown menu and select 19200. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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8. When data from the AquaMate spectrophotometer is ready to be sent to the computer, first test the connection between the spectrophotometer and data collection program. 9. Press the print key on the AquaMate spectrophotometer. The data from the AquaMate spectrophotometer should appear on the program display. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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13. Click the Start: Overwrite button on the Capture tab of the RealTerm window. The program is now ready to receive data from the AquaMate spectrophotometer and export the data to the selected file type, name and location. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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1. Power on the Orion AquaMate spectrophotometer. 2. Press the utility key to acess the instrument utility menu. 3. Press the ▲or ▼ key to highlight the Printer option and press the enter key to open the printer menu. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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2. Connect the USB printer cable to the USB port on the printer. 3. When data from the AquaMate spectrophotometer is ready to be sent to the printer, press the print key on the AquaMate spectrophotometer. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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(rear panel), USB type A port for printer (rear panel) Dimensions 30 W x 40 D x 25 H cm (11.8 x 15.7 x 9.8 in) Weight 8.6 kg (19 lb.) Power Requirements 100 to 240 V ; 50 to 60 Hz Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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8.6 kg (19 lb.) Power Requirements 100 to 240 V ; 50 to 60 Hz Note: We reserve the right to make product improvements and updates. Specifications are subject to change without notice. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Sulfide, DPD / catalyst method, tablet reagent, 50 tests AC2065 Zinc, zincon method, tablet reagent, 50 tests Visit www.thermoscientific.com/water for a complete listing of all available Thermo Scientific Orion meters, electrodes, solutions and accessories. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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(Scanning) Calculator Enables the calculator mode (Utility) Displays the vial or cuvette position placed in the light path (only with Auto 6 or Cell Position # Auto 3 sample positioner settings) Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Factor 1 Abs(WL1) x Factor = Result (Abs Ratio, Abs Difference, Multiwavelength) Enters a factor to convert a datum to a result Factor 2 Abs(WL2) x Factor = Result (Abs Ratio, Abs Diff, Multiwavelength) Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Selects a cursor point in functions using more than one cursor setting: Scan-Area Next Cursor and Scan-3-Pt Net calculations in the graph (Scanning) Number of Matched Enters the number of vials or cuvettes that will be run in the Correction Program Cuvettes (maximum of 5) Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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Concentrations the test Selects the time since the last keystroke or instrument activity; powers down the Standby unit to save lamp life (Utility) Start Wavelength Enters the beginning wavelength for a scan (Scanning) Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Scanning, Cell Growth) Removes the “>” tag of the highlighted test name to remove the test from the Unselect Test SmartStart menu (Utility Stored Tests Directory) Wavelength Enters values for the analytical wavelengths Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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3. Use the name entry screen to delete an existing custom unit, delete a character in the name of a unit, add a character to the name of a unit or accept the name of a unit. Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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Add Character function key and repeat until the desired name is shown. Press the Accept Name function key to save the entered name. 4. Press the ▲or ▼ key to highlight the custom concentration unit and press the enter key. Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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This model requires: • Slope is not equal to zero or infinity • At least one standard data point with concentration > 0 • The absorbance of the 0 concentration blank = 0A Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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If this is not the case, there will be more than one solution within the specified domain and the message “Curve cannot be used to determine sample concentrations – it may produce ambiguous results” will appear when the curve is viewed. Graph of Invalid Nonlinear Standard Curve: Orion AquaMate Spectrophotometer User Manual Thermo Scientific...
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3-Point Net Absorbance Sample Curve: 3-Point Net − Baseline correction absorbance = + � [ ] ∗ − � − �� − 3-Point Net (ASTM E16904) Thermo Scientific Orion AquaMate Spectrophotometer User Manual...
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