Limitations And How To Avoid Problems - Agilent Technologies 1260Infinity II Bio-inert FractionCollector User Manual

3
Using the Module
Configuration and Operation of the Fraction Collector

Limitations and How to Avoid Problems

Rinse Fraction Collection Needle
If Rinse Fraction Collection Needle is set to Between fraction collection, at
least 0.3 min are required to perform this task.
When doing time-based fraction collection rinsing the needle is only possible
between two time table entries, which must have a gap of at least 0.3 min. For
peak-based fraction collection a time gap of also at least 0.3 min is required. If
a new peak is detected during the rinse process, it is aborted and the needle
moves back to the next free fraction position. Depending on flow rate and
delay volume VD1 the beginning of this peak may be lost.
If you have recovery positions in your fraction collector or if you are using one
fraction collector for sample recovery in a multiple fraction collector
configuration, the rinse function between fraction collection is ignored.
Needle Movement
The option into location under Needle Movement in the fraction collector
configuration must only be used for capped 2 or 5 mL vials or well-plates.
Using other or open vials with this command can lead to a Movement failed
error.
Replacing fraction containers
When replacing filled tubes, vials or well-plates from the fraction collector
make sure to remove and re-insert the complete tray. Otherwise the fraction
collector will not recognize that the fraction containers were emptied.
Pooling
When pooling fractions, overfill protection no longer exists. It is the user's
responsibility to make sure that all fraction collection locations are large
enough to completely collect all pooled fractions. If a fraction collection
location is overfilled, an error message occurs and the pump is shut OFF.
52 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual