4
Preparing the Fraction Collector
Best Practices
Best Practices
Regular Inspections
Inspect the inlet/waste tubings and exchange them if they are worn out or
show visible signs of damage.
Power up / Shut down
Power up
• Check that the robotics is not obstructed.
Shut down
• Remove filled containers from the fraction collector after use.
• Pump a rinse solution through the fraction collector at the end of a run to
• Use recommended solvents to store the system.
Prepare the Fraction Collector
• Flush the LC system.
• Make sure to have a stable detector baseline.
• Make sure that fraction tubes are empty or that there is at least enough
Using the Fraction Collector
• Rinse the needle between runs.
• Pooling: Make sure that all fraction collection locations are large enough to
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avoid clogging.
space for the next fraction.
completely collect all pooled fractions.
1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual