Agilent Technologies 1260Infinity II Bio-inert FractionCollector User Manual page 33

When a peak is detected during a purification run (
diverter valve is triggered using the following delay time calculations:
• Start of fraction collection: t = t
• End of fraction collection: t = t
Figure 5
Performing a Delay Calibration with an UV Detector
1 Place a vial containing the Delay Sensor Calibrant (5190-8223 or
2 Remove the installed column and replace for the delay coil or union.
3 Connect a bottle of water to Channel A
4 Open a session of LAB Advisor and connect to the system with the 1260
5 Navigate to Service and Diagnostics, select Delay Volume Calibration from the
6 Click Run and follow the prompts from the Wizard.
Every Agilent 1260 Infinity detector that is used for triggering fractions has an internal
N O T E
signal delay caused by filtering the raw data. The signal delay depends on the Peakwidth
setting of the detector and is accounted for when the fraction collector is triggered.
1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual
Chromatogram from a UV-detector with peak starting at t
G1946-85020) in position 1 of the autosampler.
Infinity II Fraction Collector.
available tests.
Configuration and Operation of the Fraction Collector
+ t
0 D1
+ t + t
E D1 D2
Using the Module
Figure 5 on page 33) the
and ending at t
0
3
E
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