Page 1 Agilent InfinityLab LC Series 1260 Infinity II Analytical Fraction Collector & 1260 Infinity II Bio-inert Fraction Collector User Manual Agilent Technologies...
Page 2 Notices Warranty © Agilent Technologies, Inc. 2017 receive no greater than Restricted Rights as defined in FAR 52.227-19(c)(1-2) (June No part of this manual may be reproduced The material contained in this docu- 1987). U.S. Government users will receive in any form or by any means (including ment is provided “as is,”...
Page 3 In This Guide In This Guide This manual contains technical reference information about: • Agilent InfinityLab LC Series 1260 Infinity II Analytical Fraction Collector (G1364F) • Agilent InfinityLab LC Series 1260 Infinity II Bio-inert Fraction Collector (G5664B) 1 Introduction This chapter gives an introduction to the module and an instrument overview. 2 Site Requirements and Specifications This chapter provides information on environmental requirements, physical and performance specifications.
Page 4 In This Guide 8 Parts for Maintenance and Repair This chapter provides information on parts for maintenance and repair. 9 Identifying Cables This chapter provides information on cables used with the module. 10 Hardware Information This chapter describes the module in more detail on hardware and electronics. 11 Appendix This chapter provides additional information on safety, legal, and web.
Page 5: Table Of Contents
Contents Contents 1 Introduction Product Description Features Overview of the Module Fraction Collector Principle Leak and Waste Handling Bio-inert Materials 2 Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications 3 Using the Module Configuration and Operation of the Fraction Collector Solvent Information Turn on/off Status Indicators...
Page 6 Contents 6 Error Information What Are Error Messages General Error Messages Module Error Messages 7 Maintenance Introduction to Maintenance Warnings and Cautions Cleaning the Module Overview of Maintenance Replace the Inlet/Waste Tubings (Analytical and Bio-inert) Replace the Valve to Needle Tubing (Analytical and Bio-inert) Exchange the Analytical Needle Assembly Exchange the Needle/Capillary Carrier Assembly (Analytical and Bio-inert) Exchange the Diverter Valve...
Page 7 Early Maintenance Feedback Instrument Layout 11 Appendix General Safety Information Waste Electrical and Electronic Equipment Directive Radio Interference Sound Emission Installation of Stainless Steel Cladded PEEK Capillaries Agilent Technologies on Internet 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 8 Contents 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 9: Introduction
Overview of the Module Overview of the Analytical and Bio-inert Fraction Collector Fraction Collector Principle Leak and Waste Handling Leak and Waste Waste Concept Leak Sensor Bio-inert Materials This chapter gives an introduction to the module and an instrument overview. Agilent Technologies...
Page 10: Product Description
Introduction Product Description Product Description Product Description (G1364F) The 1260 Infinity II Analytical Fraction Collector is designed for optimized fraction collection at flow rates between 100 μL/min and 10 mL/min. This module fits within any Infinity II LC stack and is ideal for compound purification in the nanogram to milligram range using columns with internal diameters between 2.1 and 9.4 mm.
Page 11 Introduction Product Description Product Description (G5664B) The 1260 Infinity II Bio-inert Fraction Collector is the ideal extension to your system when you need to investigate separated compounds further. It fits perfectly in Agilent‘s modular LC design and does not require extra bench space.
Page 12: Features
Introduction Features Features Features (G1364F) Purification efficiency • Lowest delay volumes for minimum peak dispersion and carryover • Automated delay calibration facilitates highest fraction purity while maintaining high-precision sample recovery Instrument efficiency • Per module collection of up to 216 fractions in glass tubes with 4 outer diameters of tubes available, or in microtiter plates •...
Page 13: Overview Of The Module
Introduction Overview of the Module Overview of the Module Overview of the Analytical and Bio-inert Fraction Collector Figure 3 Overview of the Analytical and Bio-inert Fraction Collector 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 14: Fraction Collector Principle
Introduction Fraction Collector Principle Fraction Collector Principle The movements of the Fraction Collector components during the sequence are monitored continuously by the Fraction Collector processor. The processor defines specific time windows and mechanical ranges for each movement. If a specific step of the sequence is not completed successfully, an error message is generated.
Page 15: Leak And Waste Handling
Introduction Leak and Waste Handling Leak and Waste Handling Leak and Waste 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 16 Introduction Leak and Waste Handling Waste Concept 1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with 4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don't immerse in the liquid.
Page 17: Bio-Inert Materials
Bio-inert Materials Bio-inert Materials For the Bio-inert LC system, Agilent Technologies uses highest quality materials in the flow path (also referred to as wetted parts), which are widely accepted by life science scientists, as they are known for optimum inertness to biological samples and ensure best compatibility with common samples and solvents over a wide pH range.
Page 18 Introduction Bio-inert Materials Table 1 Used Bio-inert materials Module Materials Bio-inert Heat Exchangers, Valves and Capillaries: Quick-Connect Heat Exchanger Bio-inert (G7116-60009) PEEK (steel-cladded) (for Agilent 1260 Infinity II Multicolumn Thermostat G7116A) Bio-inert Valve heads (G4235A, G5631A, G5632A, G5639A) PEEK, ceramic (Al based) Bio-inert Connection capillaries Upstream of sample introduction:...
Page 19: Site Requirements And Specifications
1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications This chapter provides information on environmental requirements, physical and performance specifications. Agilent Technologies...
Page 20: Site Requirements
Site Requirements and Specifications Site Requirements Site Requirements A suitable environment is important to ensure optimal performance of the instrument. Power Considerations The module power supply has wide ranging capability. It accepts any line voltage in the range described in Table 2 on page 23.
Page 21 Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. Power cords WA R N I N G Solvents may damage electrical cables.
Page 22 Site Requirements and Specifications Site Requirements Bench Space The module dimensions and weight (see Table 2 on page 23) allow you to place the module on almost any desk or laboratory bench. It needs an additional 2.5 cm (1.0 inches) of space on either side and approximately 8 cm (3.1 inches) in the rear for air circulation and electric connections.
Page 23: Physical Specifications
Site Requirements and Specifications Physical Specifications Physical Specifications Table 2 Physical Specifications Type Specification Comments Weight 13.5 kg (29.8 lbs) w/o Thermostat Dimensions 200 x 345 × 440 mm (height × width × depth) (8 x 13.5 × 17 inches) Line voltage 100 –...
Page 24: Performance Specifications
Site Requirements and Specifications Performance Specifications Performance Specifications Performance Specifications (G1364F) Table 3 Performance Specifications 1260 Infinity II Analytical Fraction Collector (G1364F) Type Specification Comment Delay Volume (in µL) Fraction collector inlet to diverter valve: ~50 (typical, depends on length of the tubing) Diverter valve: ~15 Diverter valve to needle: ~10...
Page 25 Site Requirements and Specifications Performance Specifications Table 3 Performance Specifications 1260 Infinity II Analytical Fraction Collector (G1364F) Type Specification Comment Operating Modes Discrete fractions: default mode for all vessels. The flow is diverted to waste, while moving from one vessel position to the next vessel position Continuous flow: optional, available only when using deep well plates.
Page 26 12 x 48 mm (OD x L) tubes, ~8 mL / tube Vials, well plates, capped vials, and well plates with closing mats can be used as recommended by Agilent Technologies Maximum tube height 48 mm with long needle assembly G1367-87200...
Page 27 Site Requirements and Specifications Performance Specifications Performance Specifications (G5664B) Table 4 Performance Specifications 1260 Infinity II Bio-inert Fraction Collector (G5664B) Type Specification Comment Delay Volume (in µL) Fraction collector inlet to diverter valve: ~50 (typical, depends on length of the tubing) Diverter valve: ~15 Diverter valve to needle: ~10 Needle: ~4...
Page 28 Site Requirements and Specifications Performance Specifications Table 4 Performance Specifications 1260 Infinity II Bio-inert Fraction Collector (G5664B) Type Specification Comment Operating Modes Discrete fractions: default mode for all vessels. The flow is diverted to waste, while moving from one vessel position to the next vessel position Continuous flow: optional, available only when using deep well plates.
Page 29 12 x 48 mm (OD x L) tubes, ~8 mL / tube Vials, well plates, capped vials, and well plates with closing mats can be used as recommended by Agilent Technologies Maximum tube height 48 mm (with bio-inert needle assembly G5667-87200) Instrument Control LC and CE Drivers A.02.17 or above...
Page 30 Site Requirements and Specifications Performance Specifications 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 31: Using The Module
Setting up a Fraction Collector Method Viewing Your Results Special Applications Optimizing Fraction Collection Limitations and How to Avoid Problems Application Notes Solvent Information Turn on/off Status Indicators Agilent Local Control Modules This chapter explains the essential operational parameters of the module. Agilent Technologies...
Page 32: Configuration And Operation Of The Fraction Collector
Using the Module Configuration and Operation of the Fraction Collector Configuration and Operation of the Fraction Collector Delay Volumes and Delay Calibration Once software is installed and the fraction collector is ready to be operated, the fraction delay time needs to be determined. Figure 4 on page 32 shows a schematic drawing of the flow path between the detector and the fraction...
Page 33 Using the Module Configuration and Operation of the Fraction Collector When a peak is detected during a purification run ( Figure 5 on page 33) the diverter valve is triggered using the following delay time calculations: • Start of fraction collection: t = t •...
Page 34: Perfom A Delay Calibration Run In Openlab Cds Chemstation Edition
Using the Module Configuration and Operation of the Fraction Collector Perfom a Delay Calibration Run in OpenLAB CDS Chemstation Edition The delay calibration procedure determines the delay time between detector(s) and the fraction collector in the system. The delay is used to compensate for the time a compound needs to travel between the point of detection in the detector and the point of collection in the fraction collector.
Page 35: Help
Using the Module Configuration and Operation of the Fraction Collector Help: Start The Start page of the Delay Calibration Wizard contains a description of the fraction collector delay calibration process, together with a schematic diagram of the connections for the detector and fraction collector. Cancel Closes the Delay Calibration Wizard without consequence.
Page 36 Using the Module Configuration and Operation of the Fraction Collector Prepare Instrument for Calibration The Prepare Instrument page of the Delay Calibration Wizard leads you through the preparation of the instrument for the delay calibration run. The required preparation steps are listed in a three-column table: Activity A short description of the preparation activity.
Page 37 Using the Module Configuration and Operation of the Fraction Collector Perform Calibration Run Use the Perform Calibration page of the Delay Calibration Wizard to start the delay calibration run using the current method. The run parameters are listed; a warning is given if the method has been changed. You can switch to a different method or edit the sample information before starting the run.
Page 38 Using the Module Configuration and Operation of the Fraction Collector Finalize Calibration The Finalize Calibration page of the Delay Calibration Wizard leads you through the clean-up of the instrument back to its pre-calibration state. The required clean-up steps are listed in a three-column table: Activity A short description of the preparation activity.
Page 39 Using the Module Configuration and Operation of the Fraction Collector Recommended Calibrants Table 5 Recommended calibrants (P/N) for Agilent Fraction Collectors using ChemStation Fraction Collector type UV and MSD G1364 B/C/D/E/F, G7159B and 5190-8223 5190-8223 G7166A G5664 A/B G1946-85020 or 5190-6887 or 5190-8223 5190-8223...
Page 40: Delay Evaluation
Using the Module Configuration and Operation of the Fraction Collector Delay Evaluation The Delay Evaluation window enables you to determine the delay times/volumes between the peak detector(s) and the fraction collector. The Delay Evaluation window is split into two sections: •...
Page 41 Using the Module Configuration and Operation of the Fraction Collector Apply to Module Click the down-arrow and select the fraction collector to which to apply the calculated delay volumes. Click Apply Delay Volumes to write the delay volumes to the selected fraction collector.
Page 42: Setting Up A Fraction Collector Method
Using the Module Configuration and Operation of the Fraction Collector Setting up a Fraction Collector Method Fraction Trigger Mode Use Timetable: Enables the Timetable, but requires a timetable event. Peak-based: If Peak-based is selected, the collection of a fraction is triggered by the signal of the detector.
Page 43 Using the Module Configuration and Operation of the Fraction Collector Peak Detectors In the Peak Detectors section a list of all peak detectors that are connected to the system is displayed. Agilent InfinityLab LC Series diode array detectors, multiwavelength detectors, variable wavelength detectors and fluorescence detectors are recognized automatically.
Page 44 Using the Module Configuration and Operation of the Fraction Collector Upper Threshold At high absorbance values the light intensity on the detector is extremely low and consequently detector noise will be superimposed on the detector signal. In this case the detector noise might trigger fraction collection. To avoid false fraction collection triggering, we recommend setting an Upper Threshold well below the limit where this false triggering effect might occur.
Page 45 Using the Module Configuration and Operation of the Fraction Collector Time In the time section of the dialog box the Stoptime and the Posttime for the fraction collector can be specified. By default the Stoptime is set to as pump and the posttime is switched OFF.
Page 46 Using the Module Configuration and Operation of the Fraction Collector Fraction Preview To determine the appropriate fraction collection parameters the Agilent ChemStation provides a valuable tool that becomes accessible by pushing the button labelled Fraction Preview Tool (see Figure 7 on page 46) in the Peak Detectors section.
Page 47: Viewing Your Results
Using the Module Configuration and Operation of the Fraction Collector Viewing Your Results Data Analysis In order to display the tick marks for the collected fractions on the screen, click Signal options from the Graphics menu. Then choose Separated in the Layout dropdown list.
Page 48 Using the Module Configuration and Operation of the Fraction Collector Report In order to create reports with a fraction table and tick marks the item Add Fraction Table and Ticks in the Specify Report box has to be checked. 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 49: Special Applications
Using the Module Configuration and Operation of the Fraction Collector Special Applications Pooling We define pooling as collecting fractions from multiple injections into the same fraction locations. In the Agilent ChemStation, there are two ways to initiate pooling: • If you want to collect multiple injections from the one sample vial or sample well into the same fraction location, specify multiple injections in the Sequence Table.
Page 50 Using the Module Configuration and Operation of the Fraction Collector Sample Recovery The fraction collector offers different possibilities for sample recovery: • The preferred recovery strategy is to install two fraction collectors in your LC systems and use the last of those fraction collectors for recovery. This recovery fraction collector can be selected in the Configuration dialog box.
Page 51: Optimizing Fraction Collection
Using the Module Configuration and Operation of the Fraction Collector Using High Test Tubes in the Analytical Fraction Collector In the standard configuration of the analytical fraction collector, the maximum test tube height is limited to 48 mm. This limitation can be extended to 75 mm if •...
Page 52: Limitations And How To Avoid Problems
Using the Module Configuration and Operation of the Fraction Collector Limitations and How to Avoid Problems Rinse Fraction Collection Needle If Rinse Fraction Collection Needle is set to Between fraction collection, at least 0.3 min are required to perform this task. When doing time-based fraction collection rinsing the needle is only possible between two time table entries, which must have a gap of at least 0.3 min.
Page 53: Application Notes
Printed versions can be ordered from Agilent or pdf-files can be downloaded from the Agilent Website http://www.chem.agilent.com Table 8 Selected Agilent Technologies Application Notes Title Publication Number An optimized system for analytical and preparative work 5988-9649EN...
Page 54: Solvent Information
Using the Module Solvent Information Solvent Information Observe the following recommendations on the use of solvents. • Follow recommendations for avoiding the growth of algae, see pump manuals. • Small particles can permanently block capillaries and valves. Therefore, always filter solvents through 0.22 μm filters. •...
Page 55 Using the Module Solvent Information • Solutions of organic acids (acetic acid, formic acid, and so on) in organic solvents. For example, a 1 % solution of acetic acid in methanol will attack steel. • Solutions containing strong complexing agents (for example, EDTA, ethylene diamine tetra-acetic acid).
Page 56: Turn On/Off
Using the Module Turn on/off Turn on/off Power switch: On Turn instrument On/Off with the control software. Power switch: Off 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 57 Using the Module Turn on/off 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 58: Status Indicators
Using the Module Status Indicators Status Indicators 1 The module status indicator indicates one of six possible module conditions: Status indicators 1. Idle 2. Run mode 3. Not-ready. Waiting for a specific pre-run condition to be reached or completed. 4. Error mode - interrupts the analysis and requires attention (for example a leak or defective internal components).
Page 59: Agilent Local Control Modules
Using the Module Agilent Local Control Modules Agilent Local Control Modules Agilent InfinityLab LC Companion G7108AA The Agilent InfinityLab LC Companion gives you complete control, system monitoring, signal plotting and diagnostic capabilities for a wide range of LC system modules. The instrument control solution is available as full package including all hardware and accessories, but can also be used on your own mobile devices like tablets, mobile phones and other electronic equipment.
Page 60 Using the Module Agilent Local Control Modules 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 61: Preparing The Fraction Collector
Install Capillary Connections Setting up the Fraction Collector with the Instrument Control Interface Overview Instrument Configuration Fraction Collector User Interface (Dashboard Panel) Method Parameter Settings Advanced Settings Timetable Settings This chapter explains the operational parameters of the module. Agilent Technologies...
Page 62: Best Practices
Preparing the Fraction Collector Best Practices Best Practices Regular Inspections Inspect the inlet/waste tubings and exchange them if they are worn out or show visible signs of damage. Power up / Shut down Power up • Check that the robotics is not obstructed. Shut down •...
Page 63: Solvent Information
Preparing the Fraction Collector Solvent Information Solvent Information Observe the following recommendations on the use of solvents. • Follow recommendations for avoiding the growth of algae, see pump manuals. • Small particles can permanently block capillaries and valves. Therefore, always filter solvents through 0.22 μm filters. •...
Page 64: Material Information
Preparing the Fraction Collector Solvent Information Material Information Materials in the flow path are carefully selected based on Agilent’s experiences in developing highest quality instruments for HPLC analysis over several decades. These materials exhibit excellent robustness under typical HPLC conditions. For any special condition, please consult the material information section or contact Agilent.
Page 65 Preparing the Fraction Collector Solvent Information Polyimide Agilent uses semi-crystalline polyimide for rotor seals in valves and needle seats in autosamplers. One supplier of polyimide is DuPont, which brands polyimide as Vespel, which is also used by Agilent. Polyimide is stable in a pH range between 1 and 10 and in most organic solvents.
Page 66 Preparing the Fraction Collector Solvent Information chromatography method allows, by phosphoric acid or phosphate buffer which are less corrosive against stainless steel). • Halogenated solvents or mixtures which form radicals and/or acids, for example: → 2 COCl 2 CHCl + 2 HCl This reaction, in which stainless steel probably acts as a catalyst, occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol.
Page 67 Preparing the Fraction Collector Solvent Information Diamond-Like Carbon (DLC) Diamond-Like Carbon is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications. Fused silica and Quartz (SiO Fused silica is used in Max Light Cartridges. Quartz is used for classical flow cell windows.
Page 68 Preparing the Fraction Collector Solvent Information (HFIP). To ensure the longest possible life with HFIP, it is best to dedicate a particular chamber to this solvent, not to switch solvents, and not to let dry out the chamber. For optimizing the life of the pressure sensor, do not leave HFIP in the chamber when the unit is off.
Page 69: Capillary Color Coding Guide
Preparing the Fraction Collector Capillary Color Coding Guide Capillary Color Coding Guide Figure 10 Syntax for capillary description 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 70: Install Capillary Connections
Preparing the Fraction Collector Install Capillary Connections Install Capillary Connections For correct installation of capillary connections of the sampler it's important to choose the correct fittings, see “Capillary Color Coding Guide” on page 69. Parts required Description 5067-4650 Capillary ST 0.12 mm x 150 mm SL/SX 5067-4651 Capillary ST 0.12 mm x 280 mm SL/SX 5067-4720...
Page 71 Preparing the Fraction Collector Install Capillary Connections Carefully slide the ferrule components on after the nut Use a column or injection valve to gently tighten the and then finger-tighten the assembly while ensuring that fitting which forces the ferrule to seat onto the tubing. the tubing is completely seated in the bottom of the end fitting.
Page 72 Preparing the Fraction Collector Example of a perfect fitting Once you believe you have the fitting complete, loosen the nut, and inspect the ferrule for the correct position on the tubing. Example of a perfect fitting Examples of incorrect fittings Figure 11 Examples of incorrect fittings The first time that the swagelock fitting is used on a column or an injection valve, the...
Page 73: Setting Up The Fraction Collector With The Instrument Control Interface
Preparing the Fraction Collector Setting up the Fraction Collector with the Instrument Control Interface Setting up the Fraction Collector with the Instrument Control Interface Overview Parameters described in following sections are offered by the instrument control interface and can usually be accessed through Agilent instrument control software.
Page 74: Instrument Configuration
Preparing the Fraction Collector Setting up the Fraction Collector with the Instrument Control Interface Instrument Configuration Use the Instrument Configuration dialog box to examine and, if necessary, modify your instrument configuration. The Configurable Modules panel contains a list of all modules available for configuration. The Selected Modules panel contains the list of configured modules.
Page 75 Preparing the Fraction Collector Setting up the Fraction Collector with the Instrument Control Interface Table 9 Instrument configuration parameters Parameter Description Peak Detectors: • Module type: product number of the peak detector detected during autoconfiguration • Serial number: serial number of the peak detector detected during autoconfiguration •...
Page 76: Fraction Collector User Interface (Dashboard Panel)
Preparing the Fraction Collector Setting up the Fraction Collector with the Instrument Control Interface Fraction Collector User Interface (Dashboard Panel) Table 10 Fraction Collector User Interface Parameter Description Module graphic The items in the Fraction Collector graphic have the following meaning and function: •...
Page 77 Preparing the Fraction Collector Setting up the Fraction Collector with the Instrument Control Interface Table 10 Fraction Collector User Interface Parameter Description Context Menu The context menu of the dashboard panel contains the following commands: • Control: Displays the Fraction Collector's Control dialog box. •...
Page 78: Method Parameter Settings
Preparing the Fraction Collector Method Parameter Settings Method Parameter Settings Figure 12 Method settings The Fraction Collector method setup parameters are in eight sections: • Collection Behavior • Peak Triggers • Trigger Combinations • Stoptime • Posttime • Advanced • Timetable •...
Page 79 Preparing the Fraction Collector Method Parameter Settings Table 11 Method Parameter Settings Parameter Description Collection Behavior Use this setting to either enable or disable the fraction collection parameters of the instrument. Peak Triggers Use the Peak Triggers table to specify the detection settings of the peak detectors available in your system.
Page 80 Preparing the Fraction Collector Method Parameter Settings Table 11 Method Parameter Settings Parameter Description Trigger Combinations Use the Trigger Combinations to specify how multiple peak triggers are combined to start or stop Fraction Collection. You can choose that: • Collection of a fraction is started when all peak detectors have sent a start trigger, and continues until one detector sends a stop trigger (AND condition)
Page 81 Preparing the Fraction Collector Method Parameter Settings Table 11 Method Parameter Settings Parameter Description Advanced “Advanced Settings” on page 82 Timetable “Timetable Settings” on page 84 Fraction Preview Use the Fraction Preview screen to test the fraction collection parameters against one or more reference signals.
Page 82: Advanced Settings
Preparing the Fraction Collector Method Parameter Settings Advanced Settings Figure 13 Advanced settings The Fraction Collector method setup advanced parameters are in three sections, depending on the configuration: • Delay Settings • Fill Volume Settings • 3rd Party Pump Flow (only visible if there is no Agilent pump recognized.) 1260 Infinity II Analytical-Scale &...
Page 83 Preparing the Fraction Collector Method Parameter Settings Table 12 Advanced Parameters Description Parameter Description Delay Settings Use the Delay Settings table to specify the delay that is applied to a peak trigger signal. You can specify this setting for each peak detector separately.
Page 84: Timetable Settings
Preparing the Fraction Collector Method Parameter Settings Timetable Settings A timetable entry is crucial to enable any fraction collection. N O T E Figure 14 Timetable settings Use the Timetable to program changes in the fraction collector parameters during the analysis by entering a time in the Time field and appropriate values in the following fields of the timetable.
Page 85 Preparing the Fraction Collector Method Parameter Settings Table 13 Timetable Functions Function Parameters Fraction Mode • Off (Turns off the current fraction collection, where you use Off to turn off fraction collection at the end of the run if you have not specified a Stoptime) •...
Page 86 Preparing the Fraction Collector Method Parameter Settings Table 13 Timetable Functions Function Parameters Trigger Settings • Trigger Source (Click the down-arrow and select the trigger source from the drop-down list) • Peak Detection Mode (Click the down-arrow and select the peak detection mode from the drop-down list).
Page 87: Troubleshooting And Diagnostics
1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual Troubleshooting and Diagnostics User Interfaces Agilent Lab Advisor Software This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces. Agilent Technologies...
Page 88: User Interfaces
Troubleshooting and Diagnostics User Interfaces User Interfaces • Depending on the user interface, the available tests and the screens/reports may vary. • Preferred tool should be Agilent Lab Advisor Software, see “Agilent Lab Advisor Software” on page 89. • The Agilent OpenLAB ChemStation C.01.03 and above do not include any maintenance/test functions.
Page 89: Agilent Lab Advisor Software
Troubleshooting and Diagnostics Agilent Lab Advisor Software Agilent Lab Advisor Software The Agilent Lab Advisor Software is a standalone product that can be used with or without a chromatographic data system. Agilent Lab Advisor helps to manage the lab for high-quality chromatographic results by providing a detailed system overview of all connected analytical instruments with instrument status, Early Maintenance Feedback counters (EMF), instrument configuration information, and diagnostic tests.
Page 90 Troubleshooting and Diagnostics Agilent Lab Advisor Software 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 91: Error Information
Needle to Needle Rinse / Funnel Position Failed Needle Carrier Failed Missing Vial or Missing Well-plate Calib delay vol two peaks Valve Switch Failed Adapter Required Funnel Not Supported Pusher Wrong or Defect Wrong or Missing Needle (Analytical Scale) Initialization Failed Agilent Technologies...
Page 92 Error Information Agilent Lab Advisor Software Vessel Stuck to Needle Cluster Partner Lost During Analysis Movement to Next Position Failed Could Not Find a Valid Next Position This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions.
Page 93: What Are Error Messages
Error Information What Are Error Messages What Are Error Messages Error messages are displayed in the user interface when an electronic, mechanical, or hydraulic (flow path) failure occurs which requires attention before the analysis can be continued (for example, repair, or exchange of consumables is necessary).
Page 94: General Error Messages
Error Information General Error Messages General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well. Timeout Error ID: 0062 The timeout threshold was exceeded. Probable cause Suggested actions Check the logbook for the occurrence and The analysis was completed successfully, source of a not-ready condition.
Page 95: Remote Timeout
Error Information General Error Messages Remote Timeout Error ID: 0070 A not-ready condition is still present on the remote input. When an analysis is started, the system expects all not-ready conditions (for example, a not-ready condition during detector balance) to switch to run conditions within one minute of starting the analysis.
Page 96: Leak Sensor Short
Error Information General Error Messages Leak Sensor Short Error ID: 0082 The leak sensor in the module has failed (short circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak sensor current to change within defined limits.
Page 97: Compensation Sensor Open
Error Information General Error Messages Compensation Sensor Open Error ID: 0081 The ambient-compensation sensor (NTC) on the main board in the module has failed (open circuit). The resistance across the temperature compensation sensor (NTC) on the main board is dependent on ambient temperature. The change in resistance is used by the leak circuit to compensate for ambient temperature changes.
Page 98: Fan Failed
Error Information General Error Messages Fan Failed Error ID: 0068 The cooling fan in the module has failed. The hall sensor on the fan shaft is used by the main board to monitor the fan speed. If the fan speed falls below a certain limit for a certain length of time, the error message is generated.
Page 99: Open Cover
Error Information General Error Messages Open Cover Error ID: 0205 The top foam has been removed. Probable cause Suggested actions Please contact your Agilent service Foam not activating the sensor. representative. Please contact your Agilent service Defective sensor or main board. representative.
Page 100: Module Error Messages
Error Information Module Error Messages Module Error Messages Exhaust Fan Failed Error ID: 4456, 4457 The exhaust fan in the module has failed. The hall sensor on the fan shaft is used by the main board to monitor the fan speed.
Page 101: Side Door Error
Error Information Module Error Messages Side Door Error Error ID: 4750 The side door and/or the MTP board are damaged. This error message is not displayed before the initilization is finished. Probable cause Suggested actions Install the side door. The side door is not installed. Change the side door.
Page 102: Arm Movement Failed Or Arm Movement Timeout
Error Information Module Error Messages Arm Movement Failed or Arm Movement Timeout Error ID: 4002 The transport assembly was unable to complete a movement in one of the axes. The processor defines a certain time window for the successful completion of a movement in any particular axis.
Page 103: Needle To Needle Rinse / Funnel Position Failed
Error Information Module Error Messages Needle to Needle Rinse / Funnel Position Failed Error ID: 4955, 4980, 4981-4990 The needle failed to reach the needle rinse / funnel position. The position of the needle is monitored by a position encoder on the needle carrier.
Page 104: Needle Carrier Failed
Error Information Module Error Messages Needle Carrier Failed Error ID: The needle carrier on the transport unit assembly failed to move correctly. Probable cause Suggested actions Exchange the needle carrier assembly. Defective position sensor in the needle carrier assembly. Perform a self-alignment. Bad needle carrier positioning in X or Theta.
Page 105: Calib Delay Vol Two Peaks
Error Information Module Error Messages Calib delay vol two peaks Error ID: 4759 Two peaks have been detected during the delay calibration. Probable cause Suggested actions Check method and delay calibration procedure. Wrong sample has been used for the delay calibration.
Page 106: Adapter Required
Error Information Module Error Messages Adapter Required Error ID: 4961 The wellplate adapter is required for the following operation. Probable cause Suggested actions Attach wellplate adapter. Wellplate adapter not attached. Check cable between needle carrier assembly Cable between needle carrier assembly to to transport unit assembly.
Page 107: Pusher Wrong Or Defect
Error Information Module Error Messages Pusher Wrong or Defect Error ID: 4965 Probable cause Suggested actions Attach wellplate adapter. Wellplate adapter not attached. Exchange needle carrier assembly. Needle carrier assembly defective. Wrong or Missing Needle (Analytical Scale) Error ID: 4966 Probable cause Suggested actions Check which needle has been installed.
Page 108: Initialization Failed
Error Information Module Error Messages Initialization Failed Error ID: 4950 The fraction collector failed to complete initialization correctly. The fraction collector initialization procedure moves the needle arm and transport assembly to their home positions in a predefined routine. During initialization, the processor monitors the position sensors and motor encoders to check for correct movement.
Page 109 Error Information Module Error Messages Vessel Stuck to Needle Error ID: 4453 The vessel sticks to the needle when the needle moves up. Probable cause Suggested actions Check that the closing mat is not too thick. Closing mat to rigid/thick. Please contact your Agilent service Bad X or Theta positioning and the needle representative.
Page 110 Error Information Module Error Messages Movement to Next Position Failed Error ID: 4957 The transport mechanism detected an unexpected situation during the movement to the next fraction position. Probable cause Suggested actions Check the configuration and the loading of the Mismatch between tray configuration and tray.
Page 111: Maintenance
Exchange the Needle/Capillary Carrier Assembly (Analytical and Bio-inert) Exchange the Diverter Valve Exchange the Internal Tray Repair or Exchange a Funnel of the Internal Tray Exchange the Leak Sensor Replace the Module Firmware This chapter describes the maintenance of the module. Agilent Technologies...
Page 112: Introduction To Maintenance
Maintenance Introduction to Maintenance Introduction to Maintenance The module is designed for easy maintenance. Maintenance can be done from the front with module in place in the system stack. There are no serviceable parts inside. N O T E Do not open the module. 1260 Infinity II Analytical-Scale &...
Page 113: Warnings And Cautions
Maintenance Warnings and Cautions Warnings and Cautions Toxic, flammable and hazardous solvents, samples and reagents WA R N I N G The handling of solvents, samples and reagents can hold health and safety risks. ➔ When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.
Page 114 Maintenance Warnings and Cautions Safety standards for external equipment C AU T I O N ➔ If you connect external equipment to the instrument, make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment.
Page 115: Cleaning The Module
Maintenance Cleaning the Module Cleaning the Module To keep the module case clean, use a soft cloth slightly dampened with water, or a solution of water and mild detergent. Liquid dripping into the electronic compartment of your module can cause shock WA R N I N G hazard and damage the module ➔...
Page 116: Overview Of Maintenance
Maintenance Overview of Maintenance Overview of Maintenance The procedures described in this section can be done with the fraction collector in place in the stack. These procedures can be done on a more frequent basis. Table 14 Simple repair procedures (Analytical and Bio-inert Fraction Collectors) Procedure Typical Frequency Notes...
Page 117: Replace The Inlet/Waste Tubings (Analytical And Bio-Inert)
Maintenance Replace the Inlet/Waste Tubings (Analytical and Bio-inert) Replace the Inlet/Waste Tubings (Analytical and Bio-inert) For bio-inert modules use bio-inert parts only! When • When contaminated, worn out or visibly damaged • Typically once every year Parts required Description G1364-68602 Tubing Kit 1 –...
Page 118 Maintenance Replace the Inlet/Waste Tubings (Analytical and Bio-inert) Liquid spills or fraction losses C AU T I O N Worn or damaged tubings can cause potential spills or lead to fraction losses. ➔ Explicitly follow the described installation procedures to maximize the lifetime of inlet / waste tubing assembly and the valve to needle tubing, and to avoid potential spills or fraction losses.
Page 119 Maintenance Replace the Inlet/Waste Tubings (Analytical and Bio-inert) Remove the leak adapter from the module housing. Remove the unions from the inner tubing kit. Remove the clip by pushing it down through the module Remove the inner tubing kit by pulling it down through housing.
Page 120 Maintenance Replace the Inlet/Waste Tubings (Analytical and Bio-inert) Install the leak adapter to the module housing. Connect the outer tubing kit to the unions. Clip the inlet / waste tubing assembly into the leak A color coded ring on one of the tubings and the valve adapter.
Page 121 Maintenance Replace the Inlet/Waste Tubings (Analytical and Bio-inert) Next Steps: Re-install the tray(s) in the tray base. Start the instrument. Close the front cover. 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 122: Replace The Valve To Needle Tubing (Analytical And Bio-Inert)
Maintenance Replace the Valve to Needle Tubing (Analytical and Bio-inert) Replace the Valve to Needle Tubing (Analytical and Bio-inert) For bio-inert modules use bio-inert parts only! When • When contaminated, worn out or visibly damaged • Typically once every year Tools required Description 8710-1534...
Page 123 Maintenance Replace the Valve to Needle Tubing (Analytical and Bio-inert) Liquid spills or fraction losses C AU T I O N Worn or damaged tubings can cause potential spills or lead to fraction losses. ➔ Explicitly follow the described installation procedures to maximize the lifetime of inlet / waste tubing assembly and the valve to needle tubing, and to avoid potential spills or fraction losses.
Page 124 Maintenance Replace the Valve to Needle Tubing (Analytical and Bio-inert) Slide the tubing through the hole in the needle carrier Install the new valve to needle tubing assembly by assembly (from bottom to top) (1.) and out of the holder clipping it in to the holder in the z-arm assembly (1.), and in the z-arm assembly (2.).
Page 125: Exchange The Analytical Needle Assembly
Maintenance Exchange the Analytical Needle Assembly Exchange the Analytical Needle Assembly For bio-inert modules use bio-inert parts only! When • When the needle is visibly damaged • When the needle is blocked or contaminated Tools required Description 8710-1534 Wrench, open end, 4 mm 8710-0510 Wrench open 1/4 —...
Page 126 Maintenance Exchange the Analytical Needle Assembly Liquid spills or fraction losses C AU T I O N Worn or damaged tubings can cause potential spills or lead to fraction losses. ➔ Explicitly follow the described installation procedures to maximize the lifetime of inlet / waste tubing assembly and the valve to needle tubing, and to avoid potential spills or fraction losses.
Page 127 Maintenance Exchange the Analytical Needle Assembly Hold the needle assembly between your thumb and Insert the new needle assembly into the holder of the forefinger, slide out the assembly towards the rear of the needle carrier assembly. Make sure to push it all the way needle carrier assembly.
Page 128: Exchange The Needle/Capillary Carrier Assembly (Analytical And Bio-Inert)
Maintenance Exchange the Needle/Capillary Carrier Assembly (Analytical and Bio-inert) Exchange the Needle/Capillary Carrier Assembly (Analytical and Bio-inert) When If defective Tools required Description 8710-2438 Hex key 2.0 mm Parts required Description G1364-60022 Needle carrier assembly, analytical scale Preparations • Position the transport unit of the fraction collector in the Home position. •...
Page 129 Maintenance Exchange the Needle/Capillary Carrier Assembly (Analytical and Bio-inert) Using the 4 mm wrench and the 5/16“ wrench for Hold the needle/capillary guiding assembly between counter-holding, unscrew the valve to needle tubing your thumb and forefinger and slide out the assembly from the needle.
Page 130 Maintenance Exchange the Needle/Capillary Carrier Assembly (Analytical and Bio-inert) Slide out the needle carrier assembly to the bottom. Install the new carrier assembly and secure it with its three holding screws. The carrier is held in position with a pin / slot - fit. Push it all the way up to the top for correct orientation and installation.
Page 131 Maintenance Exchange the Needle/Capillary Carrier Assembly (Analytical and Bio-inert) Next Steps: Re-install the tray(s) in the tray base. Start the instrument. Close the front cover. 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 132: Exchange The Diverter Valve
Maintenance Exchange the Diverter Valve Exchange the Diverter Valve For bio-inert modules use bio-inert parts only! When If leaky or defective Tools required Description 8710-2438 Hex key 2.0 mm Parts required Description G5664-60901 Diverter valve Preparations • Position the transport unit of the fraction collector in the Home position. •...
Page 133 Maintenance Exchange the Diverter Valve Locate the diverter valve with the finger tight fittings of Unscrew the two finger-tight fittings of the inner tubing the inner tubing kit. kit at the diverter valve. Unscrew the finger-tight fitting of the valve to needle Disconnect the power cable of the diverter valve from tubing assembly at the diverter valve.
Page 134 Maintenance Exchange the Diverter Valve Install the valve to needle tubing at the new diverter A color coded ring on one of the tubings and the valve valve. body indicates, which tubing belongs to which port. Next Steps: Connect the finger-tight fittings of the inner tubing kit to the ports of the diverter valve.
Page 135: Exchange The Internal Tray
Maintenance Exchange the Internal Tray Exchange the Internal Tray When If defective Parts required Description G1364-63123 Internal tray preparative scale G1364-63124 Internal tray analytical scale Preparations • Position the transport unit of the fraction collector in the Home position. • Remove all installed trays from the tray base. •...
Page 136 Maintenance Exchange the Internal Tray Remove the waste tubing from the front of the Install the new tray by sliding it into position underneath instrument (1.) and slide the internal tray’s waste tubing the metal leash that holds it. The waste tubing from the to the rear of the outlet (2.) before sliding the tray out.
Page 137: Repair Or Exchange A Funnel Of The Internal Tray
Maintenance Repair or Exchange a Funnel of the Internal Tray Repair or Exchange a Funnel of the Internal Tray When When leaky or contaminated Parts required Description G1364-68730 Funnel seal kit (pack of 10) G1364-43201 Funnel coupler G1364-86708 Waste tubing kit 0.5T (analytical scale) 5022-2200 Funnel Preparations...
Page 138 Maintenance Repair or Exchange a Funnel of the Internal Tray Exploded view for the parts of the internal tray. Once the Insert the funnel into the tray and screw it tight funnel has been removed from the internal tray, it can be (clockwise).
Page 139: Exchange The Leak Sensor
Maintenance Exchange the Leak Sensor Exchange the Leak Sensor When If defective Parts required Description 5061-3356 Leak Sensor Assembly Preparations • Position the transport unit of the fraction collector in the Home position. • Remove all installed trays from the tray base. •...
Page 140 Maintenance Exchange the Leak Sensor Install the new leak sensor into its position in the leak Route the leak sensor cable nicely into the recess at the plane. front of the instrument. Plug the leak sensor into its connector on the SLF board. Next Steps: Re-install the front tray guide and fasten it with the three screws.
Page 141: Replace The Module Firmware
Maintenance Replace the Module Firmware Replace the Module Firmware When The installation of newer firmware might be necessary • if a newer version solves problems of older versions or • to keep all systems on the same (validated) revision. The installation of older firmware might be necessary •...
Page 142 Maintenance Replace the Module Firmware 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 143: Parts For Maintenance And Repair
Supported Trays for a Fraction Collector List of Recommended Test Tubes List of Recommended Vials and Caps List of Recommended Plates and Closing Mats Fraction Collector Accessory Kits Fraction Collector Thermostat This chapter provides information on parts for maintenance and repair. Agilent Technologies...
Page 144: Analytical-Scale Fraction Collector Parts
Parts for Maintenance and Repair Analytical-Scale Fraction Collector Parts Analytical-Scale Fraction Collector Parts Analytical Fraction Collector Main Assemblies Item Description G1364-63124 Internal tray analytical scale G5664-60901 Diverter valve G1367-87200 Analytical needle assembly G1364-87202 Analytical needle assembly (for extended flow rates and high test tubes) G1364-60022 Needle carrier assembly, analytical scale G1364-43701...
Page 145: Needle Assemblies
Parts for Maintenance and Repair Analytical-Scale Fraction Collector Parts Needle Assemblies Description G1364-87202 Analytical needle assembly (for extended flow rates and high test tubes) G1367-87200 Analytical needle assembly 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 146: Tubing Kits
Parts for Maintenance and Repair Analytical-Scale Fraction Collector Parts Tubing Kits Description G1364-68602 Tubing Kit 1 – 5 mL/min, 0.25 mm ID Standard tubing kit for Analytical and Bio-inert Fraction Collector G1364-68601 Tubing Kit 1 mL/min, 0.15 mm ID (OPTIONAL) G1364-68603 Tubing Kit 4 –...
Page 147: Internal Tray Assembly
Parts for Maintenance and Repair Analytical-Scale Fraction Collector Parts Internal Tray Assembly Item Description G1364-63124 Internal tray analytical scale 5022-2200 Funnel G1364-68730 Funnel seal kit (pack of 10) G1364-43201 Funnel coupler Flow delay sensor Tray internal G1364-86708 Waste tubing kit 0.5T (analytical scale) 1260 Infinity II Analytical-Scale &...
Page 148: Bio-Inert Fraction Collector Parts
Parts for Maintenance and Repair Bio-inert Fraction Collector Parts Bio-inert Fraction Collector Parts Bio-inert Fraction Collector Main Assemblies For bio-inert modules use bio-inert parts only! Item Description G1364-63124 Internal tray analytical scale G5664-60901 Diverter valve G5667-87200 Needle assembly (bio-inert) G1364-60022 Needle carrier assembly, analytical scale G1364-43701 Tray compartment divider...
Page 149 Parts for Maintenance and Repair Bio-inert Fraction Collector Parts 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 150: Needle Assembly
Parts for Maintenance and Repair Bio-inert Fraction Collector Parts Needle Assembly For bio-inert modules use bio-inert parts only! Description G5667-87200 Needle assembly (bio-inert) 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 151: Tubing Kit
Parts for Maintenance and Repair Bio-inert Fraction Collector Parts Tubing Kit Description G1364-68602 Tubing Kit 1 – 5 mL/min, 0.25 mm ID Standard tubing kit for Analytical and Bio-inert Fraction Collector G1364-68601 Tubing Kit 1 mL/min, 0.15 mm ID (OPTIONAL) G1364-68603 Tubing Kit 4 –...
Page 152: Internal Tray Assembly
Parts for Maintenance and Repair Bio-inert Fraction Collector Parts Internal Tray Assembly Item Description G1364-63124 Internal tray analytical scale 5022-2200 Funnel G1364-68730 Funnel seal kit (pack of 10) G1364-43201 Funnel coupler Flow delay sensor Tray internal G1364-86708 Waste tubing kit 0.5T (analytical scale) 1260 Infinity II Analytical-Scale &...
Page 153: Diverter-Valve Assembly
Parts for Maintenance and Repair Diverter-Valve Assembly Diverter-Valve Assembly For bio-inert modules use bio-inert parts only! Description G5664-60901 Diverter valve 0515-1211 PIN screw 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 154: Supported Trays For A Fraction Collector
Parts for Maintenance and Repair Supported Trays for a Fraction Collector Supported Trays for a Fraction Collector For more information on trays, well-plates and collecting tubes for an LC Fraction Collector, refer to the Agilent Web site: • http://www.chem.agilent.com/store/en_US/LCat-SubCat2ECS_31825/Collecting-Tubes-and-Trays • http://www.chem.agilent.com/store/en_US/LCat-SubCat2ECS_31826/Well-Plates-and-Trays Description G1364-84541...
Page 155: List Of Recommended Test Tubes
Parts for Maintenance and Repair List of Recommended Test Tubes List of Recommended Test Tubes Table 15 Round Bottom Test Tubes outer diameter height Volume recommended Part Number tray type 30 mm 100 mm 45 ml G1364-84503 (40 5042-6459 tubes) (100/pk) 30 mm 48 mm...
Page 156: List Of Recommended Vials And Caps
Parts for Maintenance and Repair List of Recommended Vials and Caps List of Recommended Vials and Caps Crimp Top Vials Caps for Use with the Analytical Scale Fraction Collector, only! N O T E Description 5181-3375 Crimp Top Vial, 2 mL, clear glass, 100/Pack 5183-4491 Crimp Top Vial, 2 mL, clear glass, 1000/Pack 5182-0543...
Page 157 Parts for Maintenance and Repair List of Recommended Vials and Caps SnapTop Vials Caps for Use with the Analytical Scale Fraction Collector, only! N O T E Description 5182-0544 Snap Top Vial, 2 mL, clear glass, 100/Pack 5183-4504 Snap Top Vial, 2 mL, clear glass, 1000/Pack 5183-4507 Snap Top Vial, 2 mL, clear glass, 100/Pack (silanized) 5182-0546...
Page 158 Parts for Maintenance and Repair List of Recommended Vials and Caps Crimp Caps Caps for Use with the Analytical Scale Fraction Collector, only! N O T E Description 5181-1210 Crimp Cap, silver aluminum, septum (clear PTFE/red rubber), 100/Pack 5183-4498 Crimp Cap, silver aluminum, septum (clear PTFE/red rubber), 1000/Pack 5181-1215 Crimp Cap, blue aluminum, septum (clear PTFE/red rubber),...
Page 159 Parts for Maintenance and Repair List of Recommended Vials and Caps Screw Caps Caps for Use with the Analytical Scale Fraction Collector, only! N O T E Description 5182-0717 Screw Cap, blue polypropylene, septum (clear PTFE/red rubber), 100/Pack 5182-0718 Screw Cap, green polypropylene, septum (clear PTFE/red rubber), 100/Pack 5182-0719 Screw Cap, red polypropylene, septum (clear PTFE/red rubber),...
Page 160: List Of Recommended Plates And Closing Mats
Parts for Maintenance and Repair List of Recommended Plates and Closing Mats List of Recommended Plates and Closing Mats Warnings Explosive gas mixtures WA R N I N G There is a risk of explosive gas mixtures in the instrument if flammable solvents are used.
Page 161 Parts for Maintenance and Repair List of Recommended Plates and Closing Mats Recommended Plates and Closing Mats (Std. Well Plates and Closing Mats for Use with the Analytical Scale Fraction Collector, only!) Description 5042-1386 96 well plate 0.5 ml, PP (pack of 10) 5042-1385 96 well plate 0.5 ml, PP (pack of 120) 5042-6454...
Page 162: Fraction Collector Accessory Kits
Parts for Maintenance and Repair Fraction Collector Accessory Kits Fraction Collector Accessory Kits Description G1364-68755 Accessory Kit 0100-1711 Male Nut PK 1/16" x 10-32 0100-1856 3/16 ID Tube Barb Y Ftg PP/OBS 5042-6421 Micro Clamp 5042-6472 Clamp Waste Tube 5063-6527 Tubing assembly, i.d.
Page 163: Fraction Collector Thermostat
Parts for Maintenance and Repair Fraction Collector Thermostat Fraction Collector Thermostat Item Description G1330-69030 FRC thermostat, exchange assembly Figure 15 Fraction Collector Thermostat 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 164 Parts for Maintenance and Repair Fraction Collector Thermostat 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 165: Identifying Cables
1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual Identifying Cables Cable Overview Analog Cables Remote Cables CAN/LAN Cables RS-232 Cables This chapter provides information on cables used with the module. Agilent Technologies...
Page 166: Cable Overview
Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent 35900A A/D converter...
Page 167 Identifying Cables Cable Overview LAN cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection) RS-232 cables (not for FUSION Description board) RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female).
Page 168: Analog Cables
Identifying Cables Analog Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules. The other end depends on the instrument to which connection is being made. Agilent Module to 35900 A/D converters p/n 35900-60750 35900 Pin Agilent...
Page 169 Identifying Cables Analog Cables Agilent Module to BNC Connector p/n 8120-1840 Pin BNC Pin Agilent Signal Name module Shield Shield Analog - Center Center Analog + Agilent Module to General Purpose p/n 01046-60105 Pin Agilent Signal Name module Not connected Black Analog - Analog +...
Page 170: Remote Cables
Identifying Cables Remote Cables Remote Cables ERI (Enhanced Remote Interface) • 5188-8029 ERI to general purpose (D-Sub 15 pin male - open end) • 5188-8044 ERI to ERI (D_Sub 15 pin male - male) • 5188-8059 ERI-Extension-Cable 1.2 m (D-Sub15 pin male / female) p/n 5188-8029 Color code Enhanced...
Page 171 Identifying Cables Remote Cables • 5188-8045 ERI to APG (Connector D_Subminiature 15 pin (ERI), Connector D_Subminiature 9 pin (APG)) p/n 5188-8045 Pin (ERI) Signal Pin (APG) Active (TTL) Start Request Stop Ready High Power on High Future Shut Down Start Prepare Ground Cable Shielding...
Page 172 Ground Cable Shielding One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 173 Identifying Cables Remote Cables Agilent Module to Agilent 35900 A/D Converters p/n 5061-3378 Pin 35900 Pin Agilent Signal Name Active module (TTL) 1 - White 1 - White Digital ground 2 - Brown 2 - Brown Prepare run 3 - Gray 3 - Gray Start 4 - Blue...
Page 174: Can/Lan Cables
Identifying Cables CAN/LAN Cables CAN/LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors. CAN Cables Description 5181-1516 CAN cable, Agilent module to module, 0.5 m 5181-1519 CAN cable, Agilent module to module, 1 m LAN Cables Description 5023-0203...
Page 175: Rs-232 Cables
Identifying Cables RS-232 Cables RS-232 Cables Description RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It's also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.
Page 176: Usb
Identifying Cables To connect a USB Flash Drive use a USB OTG cable with Mini-B plug and A socket. Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) 1260 Infinity II Analytical-Scale &...
Page 177: Hardware Information
Serial Number Information (ALL) Interfaces Overview Interfaces ERI (Enhanced Remote Interface) USB (Universal Serial Bus) Setting the 6-bit Configuration Switch Special Settings Early Maintenance Feedback Instrument Layout This chapter describes the module in more detail on hardware and electronics. Agilent Technologies...
Page 178: Firmware Description
Hardware Information Firmware Description Firmware Description The firmware of the instrument consists of two independent sections: • a non-instrument specific section, called resident system • an instrument specific section, called main system Resident System This resident section of the firmware is identical for all Agilent 1100/1200/1220/1260/1290 series modules.
Page 179 Hardware Information Firmware Description Firmware Updates Firmware updates can be done with the Agilent Lab Advisor software with files on the hard disk (latest version should be used). Required tools, firmware and documentation are available from the Agilent web: http://www.agilent.com/en-us/firmwareDownload?whid=69761 The file naming conventions are: PPPP_RVVV_XXX.dlb, where •...
Page 180 Hardware Information Firmware Description Some modules are limited in downgrading due to their main board version or their initial N O T E firmware revision. For example, a G1315C DAD SL cannot be downgraded below firmware revision B.01.02 or to a A.xx.xx. Some modules can be re-branded (e.g.
Page 181: Electrical Connections
There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply. Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations.
Page 182: Rear View Of The Module
Hardware Information Electrical Connections Rear View of the Module Figure 17 Fraction collector electrical connections 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 183: Serial Number Information (All)
Hardware Information Electrical Connections Serial Number Information (ALL) The serial number information on the instrument labels provide the following information: CCXZZ00000 Format Country of manufacturing • DE = Germany • JP = Japan • CN = China Alphabetic character A-Z (used by manufacturing) Alpha-numeric code 0-9, A-Z, where each combination unambiguously denotes a module (there can be more than one code for the same module)
Page 184: Interfaces
Hardware Information Interfaces Interfaces The Agilent InfinityLab LC Series modules provide the following interfaces: Table 17 Agilent InfinityLab LC Series Interfaces Module RS-232 Analo Special (on-board) (A) / ERI (E) Pumps G7104A/C G7110B G7111A/B, G5654A G7112B G7120A G7161A/B Samplers G7129A/B/C G7167B/C, G5667A G7157A Detectors...
Page 185 Hardware Information Interfaces Table 17 Agilent InfinityLab LC Series Interfaces Module RS-232 Analo Special (on-board) (A) / ERI (E) Fraction Collectors G7159B G7166A Requires a host module with on-board LAN with minimum FW B.06.40 or C.06.40, or with additional G1369C LAN Card G1364E/F, G5664B THERMOSTAT for G1330B...
Page 186: Overview Interfaces
Hardware Information Interfaces Overview Interfaces The CAN is inter-module communication interface. It is a 2-wire serial bus system supporting high speed data communication and real-time requirement. The modules have either an interface slot for an LAN card (e.g. Agilent G1369B/C LAN Interface) or they have an on-board LAN interface (e.g. detectors G1315C/D DAD and G1365C/D MWD).
Page 187 Remote (ERI) The ERI (Enhanced Remote Interface) connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. It allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
Page 188 Hardware Information Interfaces Table 18 ERI signal distribution Signal Description START REQUEST (L) Request to start injection cycle (for example, by start key on any module). Receiver is the autosampler. STOP (L) Request to reach system ready state as soon as possible (for example, stop run, abort or finish and stop injection).
Page 189: Eri (Enhanced Remote Interface)
Hardware Information Interfaces ERI (Enhanced Remote Interface) ERI replaces the AGP Remote Interface that is used in the HP 1090/1040/1050/1100 HPLC systems and Agilent 1100/1200/1200 Infinity HPLC modules. All new InfinityLab LC Series products using the FUSION core electronics use ERI. This interface is already used in the Agilent Universal Interface Box 2 (UIB2) ERI Description The ERI interface contains eight individual programmable input/output pins.
Page 190 Hardware Information Interfaces Enhanced Remote IO 1 (START REQUEST) IO 2 (STOP) IO 3 (READY) IO 4 (POWER ON) IO 5 (NOT USED) IO 6 (SHUT DOWN) IO 7 (START) IO 8 (PREPARE) 1 wire DATA DGND +5 V ERI out PGND PGND +24 V ERI out...
Page 191: Usb (Universal Serial Bus)
Hardware Information Interfaces 5V Distribution (Future Use) • Available directly after turn on oft the hosting module (assures that certain base functionality of the device can be detected by firmware). • For digital circuits or similar. • Provided 500 mA maximum. •...
Page 192: Setting The 6-Bit Configuration Switch
Hardware Information Setting the 6-bit Configuration Switch Setting the 6-bit Configuration Switch The 6-bit configuration switch is located at the rear of the module with FUSION electronics. Switch settings provide configuration parameters for LAN and instrument specific initialization procedures. All modules with FUSION electronics: •...
Page 193 Hardware Information Setting the 6-bit Configuration Switch Table 19 6-bit Configuration Switch Mode Function/Setting Switch 1 Switch 2 Switch 3 Switch 4 Switch 5 Switch 6 n.a. LAN Init Mode n.a. n.a. Use Default IP Address Use Stored IP Address Use DHCP to request IP Address Test System...
Page 194: Special Settings
Hardware Information Setting the 6-bit Configuration Switch Special Settings Boot-Resident/Main Firmware update procedures may require this mode in case of firmware loading errors (main/resident firmware part). If you use the following switch settings and power the instrument up again, the instrument firmware stays in the resident/main mode. In resident mode, it is not operable as a module.
Page 195 Hardware Information Setting the 6-bit Configuration Switch Table 20 Boot Resident / Forced Coldstart Init Mode Boot Main System / Keep Data Boot Resident System / Keep Data Boot Main System / Revert to Default Data Boot Resident System / Revert to Default Data Note: The setting '0' (down) is essential.
Page 196: Early Maintenance Feedback
Hardware Information Early Maintenance Feedback Early Maintenance Feedback Maintenance requires the exchange of components which are subject to wear or stress. Ideally, the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions, and not on a predefined time interval.
Page 197: Instrument Layout
Hardware Information Instrument Layout Instrument Layout The industrial design of the module incorporates several innovative features. It uses Agilent’s E-PAC concept for the packaging of electronics and mechanical assemblies. This concept is based upon the use of expanded polypropylene (EPP) layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed.
Page 198 Hardware Information Instrument Layout 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 199: Appendix
Waste Electrical and Electronic Equipment Directive Radio Interference Sound Emission Installation of Stainless Steel Cladded PEEK Capillaries First Step: Finger-tight Fitting Second Step: Installation to Connector Removing Capillaries Agilent Technologies on Internet This chapter provides additional information on safety, legal, and web. Agilent Technologies...
Page 200: General Safety Information
Appendix General Safety Information General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument.
Page 201: Before Applying Power
Verify that the voltage range and frequency of your power distribution matches to the power specification of the individual instrument. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. ➔...
Page 202: Do Not Operate In An Explosive Atmosphere
Appendix General Safety Information Do Not Operate in an Explosive Atmosphere Presence of flammable gases or fumes WA R N I N G Explosion hazard ➔ Do not operate the instrument in the presence of flammable gases or fumes. Do Not Remove the Instrument Cover Instrument covers removed WA R N I N G Electrical shock...
Page 203: Solvents
Appendix General Safety Information Solvents Toxic, flammable and hazardous solvents, samples and reagents WA R N I N G The handling of solvents, samples and reagents can hold health and safety risks. ➔ When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.
Page 204: Safety Symbols
Appendix General Safety Information Safety Symbols Table 21 Symbols The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage. Indicates dangerous voltages.
Page 205 Appendix General Safety Information Table 21 Symbols Magnetic field Magnets produce a far-reaching, strong magnetic field. They could damage TVs and laptops, computer hard drives, credit and ATM cards, data storage media, mechanical watches, hearing aids and speakers. Keep magnets at least 25 mm away from devices and objects that could be damaged by strong magnetic fields.
Page 206: Waste Electrical And Electronic Equipment Directive
Appendix Waste Electrical and Electronic Equipment Directive Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC), adopted by EU Commission on 13 February 2003, is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005.
Page 207: Radio Interference
Appendix Radio Interference Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference. All cables are in compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with unscreened cables, or...
Page 208: Sound Emission
Appendix Sound Emission Sound Emission Manufacturer’s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991. This product has a sound pressure emission (at the operator position) < 70 dB. •...
Page 209: Installation Of Stainless Steel Cladded Peek Capillaries
Appendix Installation of Stainless Steel Cladded PEEK Capillaries Installation of Stainless Steel Cladded PEEK Capillaries The 1260 Infinity Bio-inert LC system uses PEEK capillaries that are cladded with stainless steel. These capillaries combine the high pressure stability of steel with the inertness of PEEK. They are used in the high pressure flow path after sample introduction (loop/needle seat capillary) through the thermostatted column compartment/heat exchangers to the column.
Page 210: Second Step: Installation To Connector
Appendix Installation of Stainless Steel Cladded PEEK Capillaries Second Step: Installation to Connector In the second step (“Second Step: Installation to Hard Connectors” page 210 or “Second Step: Installation to Soft Connectors” on page 211), a wrench is used to rotate the fitting relative to the finger-tight position by a defined angle.
Page 211 Appendix Installation of Stainless Steel Cladded PEEK Capillaries Second and subsequent installations of a capillary to a hard connector 1 When tightening the fitting for the second and subsequent times, again start from the finger-tight position (which is not necessarily a vertical wrench position) and rotate the wrench by 90 –...
Page 212 Appendix Installation of Stainless Steel Cladded PEEK Capillaries First installation of a capillary to a soft connector 1 When tightening a fitting for the first time, start from the finger-tight position (which does not necessarily need to be a vertical wrench position) and rotate the wrench by 180 –...
Page 213 Appendix Installation of Stainless Steel Cladded PEEK Capillaries Summary for Second Step Table 22 Summary for second step First installation Subsequent installations Step Hard connectors Soft connectors 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 214: Removing Capillaries
Appendix Installation of Stainless Steel Cladded PEEK Capillaries Removing Capillaries Potential damage of capillaries C AU T I O N ➔ Do not remove fittings from used capillaries. To keep the flow path free of stainless steel, the front end of the capillary is made of PEEK.
Page 215: Agilent Technologies On Internet
Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com 1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual...
Page 216 Index Index installing compensation sensor short could not find a valid next caps accessory kit position cleaning adapter required fan failed cluster partner lost during analysis Agilent Lab Advisor software ignition without cover coding Agilent Lab Advisor leak sensor open color Agilent leak sensor short...
Page 217 Index definition of physical specifications feedback power consideration general error messages replacing firmware power consumption guide materials power cords coding bio-inert color message ignition without cover radio interference remote timeout recommended calibrants humidity module firmware recommended wavelength settings replace remote (ERI) remote inlet tubings cables...
Page 218 Index target masses temperature sensor test tubes timeout troubleshooting error messages tubing kits 146, tubings replacing 122, tubing valve to needle valve to needle tubing vials voltage range waste tubings waste electrical and electronic equipment wavelength settings WEEE directive weight 1260 Infinity II Analytical-Scale &...
Page 220 • using the fraction collector, • preparing, • troubleshooting and diagnostics, • errors, • maintenance, • parts and materials, • cables, • hardware information, • safety and related information. © Agilent Technologies 2017 Printed in Germany 09/2017 *G1364-90040* *G1364-90040* G1364-90040 Agilent Technologies...

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1260 infinity ii analyticalfraction collector

Agilent Technologies 1260Infinity II Bio-inert FractionCollector User Manual

Table of Contents

1 Introduction

2 Site Requirements and Specifications

3 Using the Module

4 Preparing the Fraction Collector

6 Error Information

7 Maintenance

8 Parts for Maintenance and Repair

10 Hardware Information

11 Appendix

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