Special Applications - Agilent Technologies 1260Infinity II Bio-inert FractionCollector User Manual

Special Applications

Pooling
We define pooling as collecting fractions from multiple injections into the
same fraction locations. In the Agilent ChemStation, there are two ways to
initiate pooling:
• If you want to collect multiple injections from the one sample vial or sample
• In case you want to collect multiple injections from the several sample vials
Figure 9
Data loss due to pump shut-down
C AU T I O N
If you specify multiple injections for pooling, the overfill protection is not activated. If a
fraction collection location is overfilled, an error message occurs and the pump is shut
OFF.
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1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual
well into the same fraction location, specify multiple injections in the
Sequence Table.
or sample wells into the same fraction location, specify the same Fract. Start
for multiple samples in the Sequence Table. An example is displayed in
Figure 9 on page 49. In this sequence the sample 1 will be injected from
plate 1 position A1 of the Autosampler and fraction collection will start at
plate 1 position A1 of the fraction collector.
For the next sample 2, which is injected from a different location in the high
performance autosampler, the fraction collection will start again at plate 1
position A1 of the fraction collector.
Pooling sequence
Make sure that all fraction collection locations are large enough to completely
collect all pooled fractions.
Configuration and Operation of the Fraction Collector
3
Using the Module
49