Page 1 1260 Infinity II Flexible Pumps Agilent InfinityLab LC Series User Manual 1260 Infinity II Flexible Pump User Manual...
Page 2 CAUTION beyond a notice until ble for errors or for incidental or conse- sent from Agilent Technologies, Inc. as the indicated conditions are fully governed by United States and interna- quential damages in connection with the understood and met.
Page 3 In This Guide... This manual covers: • Agilent 1260 Infinity II Flexible Pump (G7104C) • Agilent 1260 Infinity II Bio Flexible Pump (G7131C) Introduction This chapter gives an introduction to the module, instrument overview and internal connectors. Site Requirements and Specifications This chapter provides information on environmental requirements, physical and performance specifications.
Page 4 Error Information This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions. Maintenance This chapter describes the maintenance of the Agilent 1260 Infinity II Flexible Pumps. Parts and Materials for Maintenance This chapter provides information on parts for maintenance.
Page 5: Table Of Contents
Contents Introduction Agilent 1260 Infinity II Flexible Pump Agilent 1260 Infinity II Bio Flexible Pump Operating Principle Positions of the Multi Purpose Valve Leak and Waste Handling Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications Using the Module Magnets Turn on/off Status Indicators...
Page 6 How to Optimize the Performance of Your Module Delay Volume and Extra-Column Volume How to Configure the Optimum Delay Volume How to Achieve Higher Resolution Using Solvent Calibration Tables Troubleshooting and Diagnostics User Interfaces Agilent Lab Advisor Software Pump Leak Rate Test System Pressure Test Pump Self Test Error Information...
Page 7 Release a Stuck Inlet Valve Remove the Pump Head Assembly (G7104C) Remove the Pump Head Assembly (G7131C) Pump Head Maintenance (Tool Free) Install the Pump Head Assembly (G7104C) Install the Pump Head Assembly (G7131C) Replace the Outlet Valve (G7104C) Replace the Outlet Valve (G7131C) Replace the Multipurpose Valve Replace Parts of the Multipurpose Valve (G7104C) Replace Parts of the Multipurpose Valve (G7131C)
Page 8 Dynamic Host Configuration Protocol (DHCP) Link Configuration Selection Manual Configuration PC and User Interface Software Setup Appendix General Safety Information Waste Electrical and Electronic Equipment Directive Radio Interference Sound Emission Agilent Technologies on Internet 1260 Infinity II Flexible Pump User Manual...
Page 9: Introduction
Introduction Agilent 1260 Infinity II Flexible Pump Product Description Features Agilent 1260 Infinity II Bio Flexible Pump Product Description Features Operating Principle Positions of the Multi Purpose Valve Leak and Waste Handling Leak Sensor Waste Concept This chapter gives an introduction to the module, instrument overview and internal connectors.
Page 10: Agilent 1260 Infinity Ii Flexible Pump
Introduction Agilent 1260 Infinity II Flexible Pump Agilent 1260 Infinity II Flexible Pump Product Description The new Agilent 1260 Infinity II Flexible Pump improves your everyday efficiency by combining the performance of a high-pressure mixing UHPLC pump with the flexibility of a low-pressure mixing UHPLC pump. The power range of 5 mL/min with a maximum pressure up to 800 bar allows to run HPLC and UHPLC methods on the same LC system.
Page 11: Features
Introduction Agilent 1260 Infinity II Flexible Pump Features • High accuracy and precision for solvent flow and composition. • Maximum backpressure up to 800 bar and high analytical flow rates up to 5 mL/min for high chromatographic performance. • Integrated high efficiency degasser with low internal volume is based on PTFE AF technology and has a fast change-over of solvents for purging the pump.
Page 12: Agilent 1260 Infinity Ii Bio Flexible Pump
Introduction Agilent 1260 Infinity II Bio Flexible Pump Agilent 1260 Infinity II Bio Flexible Pump Product Description The 1260 Infinity II Bio Flexible Pump is a UHPLC pump comprising biocompatible components, developed for biopharma applications and for other analyses requiring high-salt and extreme-pH conditions. This pump enables quaternary solvent delivery at pressures up to 800 bar and flow rates up to 5 mL/min.
Page 13: Features
Introduction Agilent 1260 Infinity II Bio Flexible Pump Features • Biocompatible wetted parts in the solvent delivery unit ensure integrity of biomolecules and minimize unwanted surface interaction while increasing column lifetime • Biocompatible pump is resistant to corrosion from high salt concentrations and harsh cleaning procedures for increased instrument uptime, while the wide pH range offers increased flexibility •...
Page 14: Operating Principle
Introduction Operating Principle Operating Principle The pump head comprises two pump chambers in series with independent high-resolution motion control. A pressure sensor in the flow path monitors the pressure. The pump control uses this signal for minimizing the pressure ripple in order to achieve highest flow precision.
Page 15 Introduction Operating Principle Multisampler Pressure sensor Multipurpose valve Degasser Seal wash pump Weaver Pump head MCGV Inlet weaver Seal wash sensor Figure 3 The hydraulic path (example of G7104A Flexible Pump installed with a G7167B Multisampler) 1260 Infinity II Flexible Pump User Manual...
Page 16: Positions Of The Multi Purpose Valve
Introduction Positions of the Multi Purpose Valve Positions of the Multi Purpose Valve The Multipurpose Valve allows easy software controlled switching between different modes of operation. Normal Operating Mode Without Mixer In normal operating mode, the flow comes from the pump head, passes the pressure sensor and arrives at the central port of the Multipurpose Valve.
Page 17 Introduction Positions of the Multi Purpose Valve Purge Mode In purge mode, the flow is diverted to the waste container. From pump To waste Figure 5 Valve position in purge mode 1260 Infinity II Flexible Pump User Manual...
Page 18 Introduction Positions of the Multi Purpose Valve Normal Operating Mode With Jet Weaver and Optional Inline Filter In this mode, the flow passes an optional Jet Weaver and the optional inline filter. This configuration is recommended for special applications which require an increased mixing efficiency.
Page 19 Introduction Positions of the Multi Purpose Valve Filter Flush Mode This mode is used for cleaning the inline filter by back-flushing it. The flow goes to port 5, passes the inline filter in opposite direction and leaves to the waste through port 7.
Page 20: Leak And Waste Handling
Introduction Leak and Waste Handling Leak and Waste Handling The Agilent InfinityLab LC Series has been designed for safe leak and waste handling. It is important that all security concepts are understood and instructions are carefully followed. The solvent cabinet is designed to store a maximum volume of 8 L solvent. The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 2 L.
Page 21 Introduction Leak and Waste Handling Figure 8 Infinity II Leak Waste Concept (Flex Bench installation) 1260 Infinity II Flexible Pump User Manual...
Page 22 Introduction Leak and Waste Handling Figure 9 Infinity II Single Stack Leak Waste Concept (bench installation) 1260 Infinity II Flexible Pump User Manual...
Page 23: Leak Sensor
Introduction Leak and Waste Handling Figure 10 Infinity II Two Stack Leak Waste Concept (bench installation) The waste tube connected to the leak plane outlet on each of the bottom instruments guides the solvent to a suitable waste container. Leak Sensor Solvent incompatibility C AU T I O N The solvent DMF (dimethyl formamide) leads to corrosion of the leak sensor.
Page 24: Waste Concept
Introduction Leak and Waste Handling Waste Concept 1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with 4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don't immerse in the liquid. 1260 Infinity II Flexible Pump User Manual...
Page 25: Site Requirements And Specifications
Site Requirements and Specifications Site Requirements Physical Specifications Physical Specifications Agilent 12690 Infinity II Flexible Pump (G7104C) Physical Specifications Agilent 1260 Infinity II Bio Flexible Pump (G7131C) Performance Specifications Performance Specifications Agilent 1260 Infinity II Flexible Pump (G7104C) Performance Specifications Agilent 1260 Infinity II Bio Flexible Pump (G7131C) This chapter provides information on environmental requirements, physical and performance specifications.
Page 26: Site Requirements
Site Requirements and Specifications Site Requirements Site Requirements A suitable environment is important to ensure optimal performance of the instrument. Power Considerations The module power supply has wide ranging capability. It accepts any line voltage in the range described in Table 1 on page 29.
Page 27 Never use a power cord other than the one that Agilent shipped with this  instrument. Never use the power cords that Agilent Technologies supplies with this  instrument for any other equipment. Never use cables other than the ones supplied by Agilent Technologies ...
Page 28 Site Requirements and Specifications Site Requirements Bench Space The module dimensions and weight (see Table 1 on page 29) allow you to place the module on almost any desk or laboratory bench. It needs an additional 2.5 cm (1.0 inches) of space on either side and approximately 8 cm (3.1 inches) in the rear for air circulation and electric connections.
Page 29: Physical Specifications
Site Requirements and Specifications Physical Specifications Physical Specifications Physical Specifications Agilent 12690 Infinity II Flexible Pump (G7104C) Table 1 Physical Specifications G7104C Type Specification Comments Weight 16.1 kg (35.5 lbs) Dimensions 180 x 396 x 436 mm (height × width × depth) (7.1 x 15.6 x 17.2 inches) Line voltage 100 –...
Page 30: Physical Specifications Agilent 1260 Infinity Ii Bio Flexible Pump (G7131C)
Site Requirements and Specifications Physical Specifications Physical Specifications Agilent 1260 Infinity II Bio Flexible Pump (G7131C) Table 2 Physical Specifications Type Specification Comments Weight 16.8 kg (37.0 lbs) Dimensions 180 x 396 x 436 mm (height × width × depth) (7.1 x 15.6 x 17.2 inches) Line voltage 100 –...
Page 31: Performance Specifications
Site Requirements and Specifications Performance Specifications Performance Specifications Performance Specifications Agilent 1260 Infinity II Flexible Pump (G7104C) Table 3 Performance Specifications G7104C Type Specification Comments Hydraulic system Dual pistons in series pump with proprietary servo-controlled variable stroke design and smooth motion control for active damping.
Page 32 Site Requirements and Specifications Performance Specifications Table 3 Performance Specifications G7104C Type Specification Comments Composition precision < 0.15 % RSD or 0.02 min SD, whichever is greater Composition accuracy ± 0.4 % absolute At 1 mL/min for water/water with tracer Number of solvents Solvent selection valve Internal 4-solvent gradient formation valve included.
Page 33 Site Requirements and Specifications Performance Specifications Table 3 Performance Specifications G7104C Type Specification Comments Safety features and Leak detection, safe leak handling, maintenance leak output signal for shutdown of the pumping system. No hazardous voltages in major maintenance areas. Extensive diagnostics, error detection and display with Agilent Lab Advisor software.
Page 34: Performance Specifications Agilent 1260 Infinity Ii Bio Flexible Pump (G7131C)
Site Requirements and Specifications Performance Specifications Performance Specifications Agilent 1260 Infinity II Bio Flexible Pump (G7131C) Table 4 Performance Specifications Type Specification Comments Hydraulic system Dual pistons in series pump with proprietary servo-controlled variable stroke design and smooth motion control for active damping. Pump resolution step 300 pL size...
Page 35 Site Requirements and Specifications Performance Specifications Table 4 Performance Specifications Type Specification Comments Solvent selection valve Internal 4-solvent gradient formation valve included. External 2 x 12 solvent valve as option, fully integrated in the pump control interface. Integrated degassing Number of channels: 4, unit Internal volume per channel: 1.5 mL Materials in contact...
Page 36 Site Requirements and Specifications Performance Specifications Table 4 Performance Specifications Type Specification Comments GLP features Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of seal wear and volume of pumped mobile phase with pre-defined and user settable limits and feedback messages.
Page 37: Using The Module
Using the Module Magnets Turn on/off Status Indicators Best Practices Daily / Weekly tasks Power up / Shut-down the pump Prepare the pump Seal Wash How to deal with solvents Select channels for Multi-Channel Gradient Valve (MCGV) Optional Inline Filter Flushing the Filter Purging the Pump Recommendations for Bio-Compatible and Bio-Inert System...
Page 38: Magnets
Using the Module Magnets Magnets This stack exemplarily shows the magnets' positions in the modules. 1260 Infinity II Flexible Pump User Manual...
Page 39: Turn On/Off
Using the Module Turn on/off Turn on/off This procedure exemplarily shows an arbitrary LC stack configuration. Power switch: On 1260 Infinity II Flexible Pump User Manual...
Page 40 Using the Module Turn on/off Turn instrument On/Off with the control software. Power switch: Off 1260 Infinity II Flexible Pump User Manual...
Page 41: Status Indicators
Using the Module Status Indicators Status Indicators This procedure exemplarily shows an arbitrary LC stack configuration. 1 The module status indicator indicates one of six possible module conditions: Status indicators 1. Idle 2. Run mode 3. Not-ready. Waiting for a specific pre-run condition to be reached or completed.
Page 42: Best Practices
Using the Module Best Practices Best Practices Please also refer to TechNote SD-29000194, which can be downloaded from our website www.agilent.com. Daily / Weekly tasks Daily Tasks • Replace solvents and solvent bottles for mobile phases based on water/buffer. • Replace solvents and solvent bottles for organic mobile phase latest every second day.
Page 43: Power Up / Shut-Down The Pump
Using the Module Best Practices Power up / Shut-down the pump Prepare the Pump • Use fresh or different mobile phase (as required). • Purge each channel with 2.5 – 3 mL/min for 5 min. Open the manual purge valve or use the purge command, depending on the pump type. Shut Down the System •...
Page 44: Prepare The Pump
Using the Module Best Practices Prepare the pump The Agilent 1290 Infinity and 1290 Infinity II Pumps are equipped with automatic purge valves. This enables a variety of additional functions not available in Agilent Pumps with manual purge valves. It is possible to prepare the pump (set paramaters and start the functions Purge, Condition, or Prime) with the software.
Page 45 Using the Module Best Practices Conditioning may be necessary: • After a long period of standby • After running out of solvent • After service or repair Filling empty solvent lines C AU T I O N Damage to the seals Use a syringe or the Purge function to fill empty solvent lines.
Page 46 Using the Module Best Practices Figure 11 Prepare the pump (1290 Infinity). Right click on the module dashboard Select the appropriate function to start the procedure 1260 Infinity II Flexible Pump User Manual...
Page 47: Seal Wash
Using the Module Best Practices The user-optimized Prepare Pump context menu replaces the classical menu: • 1290 Infinity II Pumps Figure 12 Prepare the pump (1290 Infinity II pumps) Right click on the module dashboard Select Prepare Pump... Select the procedure and fill in adequate parameters Click Start to run the selected procedure Seal Wash Seal Wash guarantees a maximum seal life time.
Page 48: How To Deal With Solvents
Using the Module Best Practices How to deal with solvents • Use clean bottles only. • Exchange water-based solvents daily. • Select solvent volume to be used up within 1 – 2 days. • Use only HPLC-grade solvents and water filtered through 0.2 µm filters. •...
Page 49: Optional Inline Filter
Using the Module Best Practices Optional Inline Filter The pump can be equipped with an additional inline filter (Inline Filter Assembly (5067-5407) or Inline Filter Assembly Bio-Compatible (5720-0003)) with a nominal filter pore size of 0.3 µL. Advantages of the inline filter: •...
Page 50: Flushing The Filter
Using the Module Flushing the Filter Flushing the Filter For highest performance and robustness, the pump uses three solvent filters: 1 Solvent inlet filters (5041-2168) as part of Bottle Head Assembly (G7120-60007) have a large pore size of about 20 µm and filter out particles before they reach the pump.
Page 51 Using the Module Flushing the Filter 3 An optional Inline Filter connected to the Multipurpose Valve (average pore size 0.3 µm: Inline Filter Assembly (5067-5407) or Inline Filter Assembly Bio-Compatible (5720-0003)). This filter can be flushed using the graphical user interface or replaced as required (see “Replace Parts of the Inline Filter”...
Page 52 Using the Module Flushing the Filter Optional inline filter (for G7104A/C, G7131A, G4204A) The pump can be equipped with an additional inline filter (Inline Filter Assembly (5067-5407) or Inline Filter Assembly, Bio-Compatible (5720-0003)) with a nominal filter pore size of 0.3 µm. Advantages of the inline filter: •...
Page 53: Purging The Pump
Using the Module Purging the Pump Purging the Pump When the solvents have been exchanged or the pumping system has been turned off for a certain time (for example, overnight) oxygen will re-diffuse into the solvent channel between the solvent reservoir, vacuum degassing unit (when available in the system) and the pump.
Page 54 Using the Module Purging the Pump If the system has been run dry or air has diffused into the pump it might require additional steps to get rid of the air again. Following the procedure below will give the best and fastest results. 1 Change solvents to isopropanol.
Page 55: Recommendations For Bio-Compatible And Bio-Inert System
Using the Module Recommendations for Bio-Compatible and Bio-Inert System Recommendations for Bio-Compatible and Bio-Inert System • Make sure all supplies (fittings, capillaries, inline filters, columns, etc.) are bio-inert / bio-compatible. • Be aware that some columns for bio-related applications have a stainless steel case and can introduce iron and other metal ions in the flow path.
Page 56 Using the Module Recommendations for Bio-Compatible and Bio-Inert System Flushing Procedure  This procedure should be used when salt-containing mobile phases are used. It has to be performed regularly, at least once a week, or prior a long standby or off time, to remove salt deposits from the flow path and surfaces in contact with the solvents.
Page 57: Recommendations For Degassers
Using the Module Recommendations for Degassers Recommendations for Degassers Liquid inside the degasser C AU T I O N In case a low boiling point solvent condensates or there is a leak, liquid can accumulate inside the degasser chambers, and this will compromise performance.
Page 58: Solvent Information
Using the Module Solvent Information Solvent Information Observe the following recommendations on the use of solvents. • Follow recommendations for avoiding the growth of algae, see “Algae Growth in HPLC Systems” on page 70. • Small particles can permanently block capillaries and valves. Therefore, always filter solvents through 0.22 µm filters.
Page 59: Materials In Flow Path (G7314C)
Using the Module Solvent Information Materials in Flow Path (G7314C) Following materials are used in the flow path of this module: Part Materials Degasser chamber TFE/PDD copolymer, PFA (internal tubings); PEEK (inlets); FEP (tubings); ETFE (fittings) PFA (tubings), PEEK (fittings) Ultra clean tubings Microfluidic SST and Bio-Compatible Coating...
Page 60: General Information About Solvent/Material Compatibility
Using the Module Solvent Information General Information about Solvent/Material Compatibility Materials in the flow path are carefully selected based on Agilent’s experiences in developing highest-quality instruments for HPLC analysis over several decades. These materials exhibit excellent robustness under typical HPLC conditions. For any special condition, please consult the material information section or contact Agilent.
Page 61 Using the Module Solvent Information Polyphenylene Sulfide (PPS) Polyphenylene sulfide has outstanding stability even at elevated temperatures. It is resistant to dilute solutions of most inorganic acids, but it can be attacked by some organic compounds and oxidizing reagents. Nonoxidizing inorganic acids, such as sulfuric acid and phosphoric acid, have little effect on polyphenylene sulfide, but at high concentrations and temperatures, they can still cause material damage.
Page 62 Using the Module Solvent Information Polyimide is stable in a pH range between 1 and 10 and in most organic solvents. It is incompatible with concentrated mineral acids (e.g. sulphuric acid), glacial acetic acid, DMSO and THF. It is also degraded by nucleophilic substances like ammonia (e.g.
Page 63 Using the Module Solvent Information • Chromatographic grade ethers, which can contain peroxides (for example, THF, dioxane, diisopropylether). Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides. • Solutions of organic acids (acetic acid, formic acid, and so on) in organic solvents.
Page 64 Using the Module Solvent Information Gold Gold is inert to all common HPLC solvents, acids and bases within the specified pH range. It can be corroded by complexing cyanides and concentrated acids like aqua regia. Zirconium Oxide (ZrO Zirconium Oxide is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications.
Page 65: Solvent Recommendation For Agilent 1290 Infinity And 1290 Infinity Ii And 1260 Infinity Ii Flexible Pumps
Using the Module Solvent Recommendation for Agilent 1290 Infinity and 1290 Infinity II and 1260 Infinity II Flexible Pumps Solvent Recommendation for Agilent 1290 Infinity and 1290 Infinity II and 1260 Infinity II Flexible Pumps While the Agilent 1290 Infinity, 1290 Infinity II, and 1260 Infinity II Flexible Pumps guarantee a high performance with a wide variety of solvents, other solvents may cause harm to the pump or to the (U)HPLC system.
Page 66: Normal Phase Applications
Using the Module Solvent Recommendation for Agilent 1290 Infinity and 1290 Infinity II and 1260 Infinity II Flexible Pumps Normal Phase Applications Valves for Normal Phase Applications (G7104A/C, G7120A) Current passive inlet valves and outlet ball valves used with Agilent Infinity and Infinity II pumps do not work well while running nonpolar solvents for normal phase applications (for example, hexane and heptane).
Page 67: Solvent Handling
Using the Module Solvent Recommendation for Agilent 1290 Infinity and 1290 Infinity II and 1260 Infinity II Flexible Pumps Table 6 Recommended valves for normal phase applications Binary or High-Speed Pumps Quaternary or Flexible Pumps (G7120A) (G7104A/C) Inlet valves 1290 Infinity Inlet Valve Type N 1290 Infinity Quat Inlet Valve Type N (G4220-60122) (G4204-60122)
Page 68 Using the Module Solvent Recommendation for Agilent 1290 Infinity and 1290 Infinity II and 1260 Infinity II Flexible Pumps Handling of Buffers The following recommendations should be observed when using buffer solutions: • Buffers and aqueous solutions are possible sources of algae contamination, for avoiding related problems, please read “Algae Growth in HPLC Systems”...
Page 69 Using the Module Solvent Recommendation for Agilent 1290 Infinity and 1290 Infinity II and 1260 Infinity II Flexible Pumps Handling of Acetonitrile Acetonitrile is a solvent that is frequently used in reversed-phase chromatography. Despite of its common use, it can be a source of issues if not handled correctly.
Page 70: Algae Growth In Hplc Systems
Using the Module Algae Growth in HPLC Systems Algae Growth in HPLC Systems The presence of algae in HPLC systems can cause a variety of problems that may be incorrectly diagnosed as instrument or application problems. Algae grow in aqueous media, preferably in a pH range of 4-8. Their growth is accelerated by buffers, for example phosphate or acetate.
Page 71: Setting Up The Pump With The Instrument Control Interface
Using the Module Setting up the Pump with the Instrument Control Interface Setting up the Pump with the Instrument Control Interface Overview Parameters described in following sections are offered by the instrument control interface and can usually be accessed through Agilent instrument control software.
Page 72 Using the Module Setting up the Pump with the Instrument Control Interface Options: • Pressure Unit: Select the pressure units from the drop-down list (bar, psi or MPa). • Seal wash installed: This check box is marked to indicate that an optional seal wash has been detected during autoconfiguration.
Page 73: The Pump User Interface (Dashboard Panel)
Using the Module Setting up the Pump with the Instrument Control Interface The Pump User Interface (Dashboard Panel) Module Graphic The items in the pump graphic have the following meaning and function: The level of solvent in the bottle is denoted by the gray area; when the solvent level falls below the specified volume, the area turns yellow;...
Page 74 Using the Module Setting up the Pump with the Instrument Control Interface Instrument Signals The instrument signals are displayed when clicking on the icon in the upper right corner of the module dashboard. The following pump signals are displayed: Flow The current solvent flow rate (in mL/min).
Page 75 Using the Module Setting up the Pump with the Instrument Control Interface Context Menu The context menu of the dashboard panel contains the following commands: Control Displays the pump's Control dialog box. Method Displays the pump's Method Setup dialog box. Set Error Method Sets the method that is loaded if an error occurs to the method that is currently available in the hardware.
Page 76 Using the Module Setting up the Pump with the Instrument Control Interface Prepare Pump Dialog Table 7 Prepare Pump dialog Parameter Limits Description Purge Time: 0 – 100.00 min Setup and activation of Purge parameters. The automatic purge valve can in steps of 0.01.
Page 77: Control Settings
Using the Module Setting up the Pump with the Instrument Control Interface Control Settings The pump control parameters are in two sections: • Pump • Automatic Turn On Table 8 Pump control parameters Parameter Description Pump Enables you to switch the pump On, Off or to a Standby condition. In the Standby condition, the pump motor is still active, and when the pump is switched on again, it does not need to be re-initialized.
Page 78 Using the Module Setting up the Pump with the Instrument Control Interface Table 9 Method parameters Parameter Limits Description Flow 0.00 – 5.00 mL/min in The flow is the rate of movement of eluent along the column. It is important steps of 0.001.
Page 79 79 ISET Set the ISET parameters in this dialog box. For further information on ISET, please refer to the Online Help or to the Agilent InfinityLab LC Series Systems with Intelligent System Emulation Technology - User Manual (p/n G7120-90310).
Page 80 Using the Module Setting up the Pump with the Instrument Control Interface Table 10 Advanced method parameters Parameter Limits Description Minimum Stroke 20 – 100 µL The Stroke Volume is used for optimizing between performance of the module and seal life time. For performance a low stroke volume is beneficial, as it divides disturbances into smaller packages, but a larger volume is extending the life time of the pump seals.
Page 81 Using the Module Setting up the Pump with the Instrument Control Interface Timetable Settings Use the Timetable to program changes in the pump parameters during the analysis by entering a time in the Time field and appropriate values in the following fields of the timetable.
Page 82 Using the Module Setting up the Pump with the Instrument Control Interface Blend Assist The Blend Assist table allows you to blend two or more solvents or solvent mixtures from stock solutions. The blends must be of pure solvents or pure solvents with additives.
Page 83: Agilent Local Control Modules
Using the Module Agilent Local Control Modules Agilent Local Control Modules Agilent InfinityLab Companion G7108AA The Agilent InfinityLab Companion gives you complete control, system monitoring, signal plotting, and diagnostic capabilities for a wide range of LC system modules. The instrument control solution is available as full package including all hardware and accessories, but can also be used on your own mobile devices like tablets, mobile phones and other electronic equipment.
Page 84: Preparing The Pump
Preparing the Pump Capillary Coding Guide Syntax for Capillary Description At-a-glance color-coding keys Installing Capillaries This chapter provides information on how to set up the module for an analysis and explains the basic settings. 1260 Infinity II Flexible Pump User Manual...
Page 85: Capillary Coding Guide
Preparing the Pump Capillary Coding Guide Capillary Coding Guide Syntax for Capillary Description The tables below are your guide to identifying the proper specifications for your capillary. On all capillaries, dimensions are noted in id (mm), length (mm) and, where applicable, volume (µL). When you receive your capillary, these abbreviations are printed on the packaging.
Page 86 Preparing the Pump Capillary Coding Guide Table 11 Capillary coding guide Type Material Fitting left/fitting right The type gives some The material indicates The fitting left/right indication on the primary which raw material is indicate which fitting is function, like a loop or a used.
Page 87 Preparing the Pump Capillary Coding Guide At-a-glance color-coding keys The color of your capillary will help you quickly identify the capillary id. Table 12 Color-coding key for Agilent capillary tubing Internal diameter in Color code 0.015 Orange 0.025 Yellow 0.05 Beige 0.075 Black...
Page 88: Installing Capillaries
Preparing the Pump Installing Capillaries Installing Capillaries Capillaries and connections depend on which system is installed. Table 13 Capillary connections for 1260 Infinity II systems From Bottle Head Assembly (G7120-60007) Solvent Bottle Infinity II Pump Capillary ST 0.17 mm x 500 mm SI/SI (5500-1246) Pump Sampler Capillary, ST, 0.17 mm x 900 mm SI/SX (5500-1217)
Page 89 Preparing the Pump Installing Capillaries Table 14 Capillary connections for 1290 Infinity II systems From Bottle Head Assembly (G7120-60007) Solvent Bottle Infinity II Pump Capillary ST 0.17 mm x 400 mm SI/SI (5500-1245) Pump Sampler Capillary, ST, 0.17 mm x 900 mm SI/SX (5500-1217) Pump Vialsampler with ICC Capillary ST 0.12 mm x 500 mm SL/S (5500-1157)
Page 90 Preparing the Pump Installing Capillaries Table 16 Capillary connections for 1290 Infinity II Bio LC From Bottle Head Assembly (G7120-60007) Solvent Bottle Infinity II Pump Capillary MP35N 0.17 mm x 500 mm, SI/SI (5500-1419) Pump Multisampler Capillary MP35N 0.12 mm x 500 mm SI/SI (5500-1279) Multisampler Quick Connect Capillary MP35N 0.12 mm x 105 mm MCT Heat Exchanger...
Page 91 Preparing the Pump Installing Capillaries Carefully slide the ferrule components on after the nut Use a column or injection valve to gently tighten the and then finger-tighten the assembly while ensuring fitting which forces the ferrule to seat onto the tubing or that the tubing is completely seated in the bottom of the capillary.
Page 92: How To Optimize The Performance Of Your Module
How to Optimize the Performance of Your Module Delay Volume and Extra-Column Volume Delay Volume How to Configure the Optimum Delay Volume How to Achieve Higher Resolution Using Solvent Calibration Tables How it Works How to Import the Latest Solvent Calibration Tables Recommendations for Setting up Methods Using Salt-Containing Eluents This chapter gives hints on how to optimize the performance or use additional devices.
Page 93: Delay Volume And Extra-Column Volume
How to Optimize the Performance of Your Module Delay Volume and Extra-Column Volume Delay Volume and Extra-Column Volume The delay volume is defined as the system volume between the point of mixing in the pump and the front of the column. The extra-column volume is defined as the volume between the injection point and the detection point, excluding the volume in the column.
Page 94: How To Configure The Optimum Delay Volume
How to Optimize the Performance of Your Module How to Configure the Optimum Delay Volume How to Configure the Optimum Delay Volume The design of the Flexible Pump offers a strongly decreased delay volume compared to standard 600 bar pressure pumps. For the Flexible Pump, mixing is done in the multi-channel gradient valve at ambient pressure.
Page 95 How to Optimize the Performance of Your Module How to Configure the Optimum Delay Volume Figure 14 The Jet Weaver mixer The installation procedure is illustrated in “Install the Jet Weaver” on page 167. 1260 Infinity II Flexible Pump User Manual...
Page 96: How To Achieve Higher Resolution
How to Optimize the Performance of Your Module How to Achieve Higher Resolution How to Achieve Higher Resolution Increased resolution in a separation will improve the qualitative and quantitative data analysis, allow more peaks to be separated or offer further scope for speeding up the separation.
Page 97 How to Optimize the Performance of Your Module How to Achieve Higher Resolution square root of N so doubling the length of the column will increase resolution by a factor of 1.4. What is achievable depends on the viscosity of the mobile phase as this relates directly to the pressure.
Page 98 How to Optimize the Performance of Your Module How to Achieve Higher Resolution This shows that k and hence resolution can be increased by having shallower gradients (2 to 5 %/min change is a guideline), higher flow rates and smaller volume columns.
Page 99: Using Solvent Calibration Tables
How to Optimize the Performance of Your Module Using Solvent Calibration Tables Using Solvent Calibration Tables How it Works The compressibility of the mobile phase has an effect on the performance of the pump. For best flow accuracy and mixing performance, the compressibility parameter in the Method Settings of the pump shall be chosen according to the mobile phase being used.
Page 100 How to Optimize the Performance of Your Module Using Solvent Calibration Tables The procedure for importing the latest solvent calibration tables is as follows: 1 Enter the Instrument configuration of the Agilent LC Driver. 1260 Infinity II Flexible Pump User Manual...
Page 101 How to Optimize the Performance of Your Module Using Solvent Calibration Tables 2 Click on the module that needs the latest solvent calibration table (ie G7120A). Then choose configure, launching the additional configuration of the pump module. 1260 Infinity II Flexible Pump User Manual...
Page 102 How to Optimize the Performance of Your Module Using Solvent Calibration Tables 3 In the additional configuration of the pump module, choose Configure Solvent Type Catalogs… 4 In the Solvent Type Catalogs, press the Import button. 5 Navigate to the location of the downloaded Agilent Solvent Calibration Table and click the Open button.
Page 103: Recommendations For Setting Up Methods Using Salt-Containing Eluents
How to Optimize the Performance of Your Module Using Solvent Calibration Tables 6 The imported solvent table will now appear in the Solvent Type Catalogs. The imported solvent table is now available for selection in the Method Settings of the pump module. Recommendations for Setting up Methods Using Salt-Containing Eluents For LC Drivers 3.4 or newer, please use Ammonium Sulfate in Water V.04 and...
Page 104 How to Optimize the Performance of Your Module Using Solvent Calibration Tables Main salt component Total salt content (c, mol/L) Select solvent definition 0.25 < c ≤ 0.75 Sodium Chloride 0.5M V.03 0.75 < c ≤ 1.25 Sodium Chloride 1.0M V.03 NaCl 1.25 <...
Page 105: Troubleshooting And Diagnostics
Troubleshooting and Diagnostics User Interfaces Agilent Lab Advisor Software Pump Leak Rate Test Troubleshooting the Pump Leak Rate Test System Pressure Test Pump Self Test Overview about the troubleshooting and diagnostic features. 1260 Infinity II Flexible Pump User Manual...
Page 106: User Interfaces
Troubleshooting and Diagnostics User Interfaces User Interfaces • Depending on the user interface, the available tests and the screens/reports may vary. • The preferred tool for troubleshooting and diagnostics should be Agilent Lab Advisor Software, see “Agilent Lab Advisor Software” on page 107.
Page 107: Agilent Lab Advisor Software
Lab Advisor Advanced Lab Advisor Basic is included with every Agilent 1200 Infinity Series and Agilent InfinityLab LC Series instrument. The Lab Advisor Advanced features can be unlocked by purchasing a license key, and include real-time monitoring of instrument actuals, all various instrument signals, and state machines.
Page 108: Pump Leak Rate Test
Troubleshooting and Diagnostics Pump Leak Rate Test Pump Leak Rate Test The Pump Leak Rate Test is a diagnostic test to check the integrity and tightness of the pump components. The test is started from the Services & Diagnostics section in the Agilent Lab Advisor Software. The test is first evaluating the tightness from the outlet valve downstream to the purge valve.
Page 109 Troubleshooting and Diagnostics Pump Leak Rate Test Evaluation: The result as well as the applied limits are displayed after the automatic evaluation. The limits are: • The allowed leak limit for the secondary piston is ≤3 µL/min • The allowed leak limit for the primary piston is ≤30 µL/min A report can be displayed, saved or printed by opening it with the Print Result button at the lower right of the screen.
Page 110 Troubleshooting and Diagnostics Pump Leak Rate Test Figure 16 Dynamic (primary) Leak Test 1260 Infinity II Flexible Pump User Manual...
Page 111: Troubleshooting The Pump Leak Rate Test
Troubleshooting and Diagnostics Pump Leak Rate Test Troubleshooting the Pump Leak Rate Test Secondary Leak > 3 µL/min • Leak between the OBV and automatic purge valve • Check for visible leaks on fittings and connectors • Connector not fixed / tight enough •...
Page 112 Troubleshooting and Diagnostics Pump Leak Rate Test • Outlet valve not properly assembled • Retighten the outlet valve • Check the position of the gold seal • Leaky piston seals and/or position-dependent leaks on the piston • Remove the SW tubes from the support ring and check for leaks •...
Page 113: System Pressure Test
Troubleshooting and Diagnostics System Pressure Test System Pressure Test The System Pressure Test is performed to evaluate the leak tightness of the system up to the point where the system is capped off. The test is started from the Services & Diagnostics section in the Agilent Lab Advisor Software or in the Local Controller.
Page 114 Troubleshooting and Diagnostics System Pressure Test Figure 17 System Pressure Test 1260 Infinity II Flexible Pump User Manual...
Page 115: Pump Self Test
Troubleshooting and Diagnostics Pump Self Test Pump Self Test The Pump Self Test combines a Pump Leak Rate Test with several consecutive System Pressure Tests to determines the leak rates at various positions in the flow path of the Quaternary Pump. During the test either the leak rate or the flow restriction is determined, according to the run protocol, which can be configured during the test setup.
Page 116 Troubleshooting and Diagnostics Pump Self Test Pump Leakage: The pump leakage is measured when any of the other leakage tests is selected. First, a full pump leak rate test is run, which can be limited to the maximum allowed system pressure. After that a pressure test is performed. Mixer Leakage: Determines the leakage at the mixer (if installed).
Page 117 Troubleshooting and Diagnostics Pump Self Test Preparations: 1 Flush the system with HPLC grade water for several minutes from any solvent channel. 2 Start the Pump Self Test and choose the test you want to test the system with. The pressure will automatically adjust to best fit your system. 3 Choose if you want to use a blank nut or the sampler (only certain modules) to block the pump.
Page 118 Troubleshooting and Diagnostics Pump Self Test Figure 18 Pump Self Test 1260 Infinity II Flexible Pump User Manual...
Page 119: Error Information
Error Information What Are Error Messages General Error Messages Timeout Shutdown Remote Timeout Lost CAN Partner Leak Sensor Short Leak Sensor Open Compensation Sensor Open Compensation Sensor Short Fan Failed Leak Pump Error Messages Pressure of quaternary pump above upper limit Pressure below lower limit Target pressure not reached for quaternary pump degasser Solvent counter exceeded limit...
Page 120 Error Information Pump Self Test Maximum stroke is too short Pump drive stop not found Timeout: Wait for Composition Timeout: Wait for run volume Timeout: Wait for Volume Timeout: Wait for Flow Timeout: Wait for Pressure Drive Encoder failed Drive phases differ too much in electric resistance Degasser's pressure limit violation Seal wash pump was missing when tried to turn on Valve hardware overcurrent (MCGV)
Page 121: What Are Error Messages
Error Information What Are Error Messages What Are Error Messages Error messages are displayed in the user interface when an electronic, mechanical, or hydraulic (flow path) failure occurs which requires attention before the analysis can be continued (for example, repair, or exchange of consumables is necessary).
Page 122: General Error Messages
Error Information General Error Messages General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well. Timeout Error ID: 0062 The timeout threshold was exceeded. Probable cause Suggested actions Check the logbook for the occurrence and source The analysis was completed successfully, of a not-ready condition.
Page 123: Shutdown
Error Information General Error Messages Shutdown Error ID: 0063 An external instrument has generated a shutdown signal on the remote line. The module continually monitors the remote input connectors for status signals. A LOW signal input on pin 4 of the remote connector generates the error message.
Page 124: Remote Timeout
Error Information General Error Messages Remote Timeout Error ID: 0070 A not-ready condition is still present on the remote input. When an analysis is started, the system expects all not-ready conditions (for example, a not-ready condition during detector balance) to switch to run conditions within one minute of starting the analysis.
Page 125: Leak Sensor Short
Error Information General Error Messages Leak Sensor Short Error ID: 0082 The leak sensor in the module has failed (short circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak sensor current to change within defined limits.
Page 126: Leak Sensor Open
Error Information General Error Messages Leak Sensor Open Error ID: 0083 The leak sensor in the module has failed (open circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak sensor current to change within defined limits.
Page 127: Compensation Sensor Short
Error Information General Error Messages Compensation Sensor Short Error ID: 0080 The ambient-compensation sensor (NTC) on the power switch board in the module has failed (open circuit). The resistance across the temperature compensation sensor (NTC) on the power switch board is dependent on ambient temperature. The change in resistance is used by the leak circuit to compensate for ambient temperature changes.
Page 128: Fan Failed
Error Information General Error Messages Fan Failed Error ID: 0068 The cooling fan in the module has failed. The hall sensor on the fan shaft is used by the mainboard to monitor the fan speed. If the fan speed falls below a certain limit for a certain length of time, the error message is generated.
Page 129: Leak
Error Information General Error Messages Leak Error ID: 0064 A leak was detected in the module. The signals from the two temperature sensors (leak sensor and board-mounted temperature-compensation sensor) are used by the leak algorithm to determine whether a leak is present. When a leak occurs, the leak sensor is cooled by the solvent.
Page 130: Pump Error Messages
Error Information Pump Error Messages Pump Error Messages These errors are pump specific. Pressure of quaternary pump above upper limit Error ID: 29163 The pressure has exceeded the upper pressure limit. • Parameter: Measured pressure Probable cause Suggested actions • Check for blockages in the LC system, e.g.
Page 131: Pressure Below Lower Limit
Error Information Pump Error Messages Pressure below lower limit Error ID: 29176 The pressure has dropped below the lower limit. • Parameter: None Probable cause Suggested actions Check for leaks. Leak Check bottle filling. Bottle empty Check solvent. Wrong solvent (viscosity) Check flow rate and lower pressure limit.
Page 132: Solvent Counter Exceeded Limit
Error Information Pump Error Messages Solvent counter exceeded limit Error ID: 29146 The counter for the solvent volume has exceeded the limit, which has been set in the user interface. Probable cause Suggested actions Refill solvent bottle. No solvent present. Check solvent counter setting in user interface.
Page 133: Flow Rate Limit Exceeded
Error Information Pump Error Messages Flow rate limit exceeded Error ID: 29164 The flow rate of the quaternary pump has exceeded the limit, while the pump runs in pressure controlled mode, e.g. during a pressure test. • Parameter: None Probable cause Suggested actions Check for leaks in the pump and flow path.
Page 134: Reading The Pump Encoder Tag Failed
Error Information Pump Error Messages Reading the pump encoder tag failed Error ID: 29201 Reading the pump encoder tag has failed. • Parameter: 1 – 2 referring to pump drive Probable cause Suggested actions Please contact your Agilent service Defect connection between encoder and representative.
Page 135: Pump Drive Blocked Or Encoder Failed
Error Information Pump Error Messages Pump drive blocked or encoder failed Error ID: 29214 Pump drive blocked or encoder failed. • Parameter: None Probable cause Suggested actions Please contact your Agilent service Blockage of the system before pressure representative. sensor. Please contact your Agilent service Drive encoder failed.
Page 136: Drive Current Too High
Error Information Pump Error Messages Drive current too high Error ID: 29236 The current consumption of the pump drive is too high. • Parameter: 1 – 2 referring to pump drive Probable cause Suggested actions Check for blockage of e.g. outlet valve filter frit, Blockage of system before pressure sensor.
Page 137: Overcurrent Of Pump Drive
Error Information Pump Error Messages Overcurrent of pump drive Error ID: 29202 The current consumption of the pump drive is too high. • Parameter: 1 – 2 referring to pump drive Probable cause Suggested actions Check for blockage of e.g. outlet valve filter frit, Blockage of system before pressure sensor.
Page 138: Defect Connection Between Main Board And Pump Drive Encoder
Error Information Pump Error Messages Defect connection between main board and pump drive encoder Error ID: 29208 Defect connection between main board and pump drive encoder. • Parameter: 1 – 2 referring to pump drive Probable cause Suggested actions Please contact your Agilent service Defect connection between main board and representative.
Page 139: Reading Of Multipurpose Valve Tag Failed
Error Information Pump Error Messages Reading of multipurpose valve tag failed Error ID: 29240 Reading the multipurpose valve tag failed. • Parameter: None Probable cause Suggested actions Check cable connection. Reading of multipurpose valve tag failed. Multipurpose valve head tag defect or empty. Replace multipurpose valve head. Please contact your Agilent service Multipurpose valve tag reader is defect.
Page 140: Insufficient Power Of Drive Encoder Led
Error Information Pump Error Messages Insufficient power of drive encoder LED Error ID: 29235 Insufficient power of drive encoder LED. • Parameter: 1 – 2 referring to pump drive Probable cause Suggested actions Please contact your Agilent service Pump drive encoder is defect. representative.
Page 141: Unknown Multipurpose Valve Type
Error Information Pump Error Messages Unknown multipurpose valve type Error ID: 29242 The type information of the multipurpose valve is invalid. • Parameter: None Probable cause Suggested actions Check or replace multipurpose valve head. Wrong valve head installed. Check or replace multipurpose valve head. Valve head has invalid RFID tag content.
Page 142: Maximum Stroke Is Too Short
Error Information Pump Error Messages Maximum stroke is too short Error ID: 29203 The maximum stroke is too short. During initialization the pump defines the operation position of the pump drives and therefore the pistons. First the pump drive moves backwards to find a mechanical stop within the ball screw.
Page 143: Timeout: Wait For Composition
Error Information Pump Error Messages Timeout: Wait for Composition Error ID: 29180 A target condition (composition) has been sent to the instrument which should have been reached within an expected time frame but didn’t. Either the limit, time frame or the current value of the variable has been modified later directly or indirectly.
Page 144: Timeout: Wait For Volume
Error Information Pump Error Messages Timeout: Wait for Volume Error ID: 29182 A target condition (volume, which is the delivered flow since the limit has been set) has been sent to the instrument which should have been reached within an expected time frame but didn’t.
Page 145: Timeout: Wait For Pressure
Error Information Pump Error Messages Timeout: Wait for Pressure Error ID: 29185 A target condition (pressure) has been sent to the instrument which should have been reached within an expected time frame but didn’t. Either the limit, time frame or the current value of the variable has been modified later directly or indirectly.
Page 146: Drive Phases Differ Too Much In Electric Resistance
Error Information Pump Error Messages Drive phases differ too much in electric resistance Error ID: 29216 Pump drive calibration has failed due to a strong difference electric resistances for different motor phases. Probable cause Suggested actions Please contact your Agilent service Pump drive cable defect.
Page 147: Seal Wash Pump Was Missing When Tried To Turn On
Error Information Pump Error Messages Seal wash pump was missing when tried to turn on Error ID: 29223 The seal wash pump has not been detected (while being configured or detected before) Probable cause Suggested actions Defect cable connection to seal wash pump. Check cable connection. Please contact your Agilent service Defect seal wash pump motor.
Page 148: Invalid Degasser Pressure Signal
Error Information Pump Error Messages Invalid degasser pressure signal Error ID: 29253 The degasser pressure signal is invalid. Probable cause Suggested actions Please contact your Agilent service Degasser might be disconnected representative. Please contact your Agilent service Pressure sensor might be defective representative.
Page 149: Maintenance
Maintenance Introduction to Maintenance Warnings and Cautions Overview of Maintenance Cleaning the Module Install Fittings and Capillaries Remove and Install Doors Replace the Pressure Sensor Replace the Inlet Weaver Replace the Inlet Valve (G7104C) Replace the Inlet Valve (G7131C) Remove the Jet Weaver Install the Jet Weaver Replace the Seal Wash Pump Cartridge Replace the Multi-Channel Gradient Valve (MCGV)
Page 150 Maintenance Pump Error Messages Replace Parts of the Multipurpose Valve (G7104C) Replace Parts of the Multipurpose Valve (G7131C) Replace the High Pressure Outlet Filter or Filter Frit Install the Inline Filter Remove the Inline Filter Replace Parts of the Inline Filter Replace the Seal Wash Sensor Replace the Module Firmware Prepare the Pump Module for Transport...
Page 151: Introduction To Maintenance
Maintenance Introduction to Maintenance Introduction to Maintenance Figure 19 on page 151 shows the main user-accessible assemblies of the Agilent 1260 Infinity II Flexible Pump. These parts can be accessed from the front (simple repairs) and don’t require to remove the pump from the system stack. Seal Wash Sensor High Pressure Filter Housing...
Page 152 Maintenance Introduction to Maintenance Seal Wash Sensor High Pressure Filter Housing Jet Weaver (optional) Seal Wash Pump Degasser Multi-Channel Gradient Valve (MCGV) Inlet Weaver Pump Head Assembly Pressure Sensor Multipurpose Valve Figure 20 Overview of maintenance parts for the Bio Flexible Pump Recommended Interval for Preventive Maintenance The recommended interval for preventive maintenance is: •...
Page 153: Warnings And Cautions
Maintenance Warnings and Cautions Warnings and Cautions Toxic, flammable and hazardous solvents, samples and reagents WAR N IN G The handling of solvents, samples and reagents can hold health and safety risks. When working with these substances observe appropriate safety ...
Page 154: Overview Of Maintenance
Maintenance Overview of Maintenance Overview of Maintenance The following pages describe maintenance (simple repairs) of the module that can be carried out without opening the main cover. 1260 Infinity II Flexible Pump User Manual...
Page 155: Cleaning The Module
Maintenance Cleaning the Module Cleaning the Module To keep the module case clean, use a soft cloth slightly dampened with water, or a solution of water and mild detergent. Avoid using organic solvents for cleaning purposes. They can cause damage to plastic parts. Liquid dripping into the electronic compartment of your module can cause WAR N IN G shock hazard and damage the module...
Page 156: Install Fittings And Capillaries
Maintenance Install Fittings and Capillaries Install Fittings and Capillaries Solvent can spray under high pressure. WAR N IN G Observe appropriate safety procedures (for example, goggles, safety  gloves and protective clothing), when opening flow path. Deformation of fittings and seals C AU T I O N Liquid drops under high pressure act like solid parts.
Page 157: Remove And Install Doors
Maintenance Remove and Install Doors Remove and Install Doors Parts required Description 5067-5745 Door Assembly Infinity 180 Left 5067-5746 Door Assembly Infinity 180 Right The figures shown in this procedure exemplarily show the Infinity II Multisampler NOTE module. The principle of how to remove and/or install doors works in the same way for all Infinity II modules.
Page 158 Maintenance Remove and Install Doors Press the release buttons and pull the front door out. For the Installation of the front door. Insert the hinges into their guides and move the door in until the release buttons click into their final position. 1260 Infinity II Flexible Pump User Manual...
Page 159: Replace The Pressure Sensor
Maintenance Replace the Pressure Sensor Replace the Pressure Sensor When No or invalid pressure signal Tools required Description 8710-2412 Hex key 2.5 mm, 15 cm long, straight handle 8710-0510 Open-end wrench 1/4 — 5/16 inch Parts required Description 5067-6791 Pressure sensor 1300 bar 5067-7010 Pressure sensor Bio-Compatible 1300 bar Preparations...
Page 160 Maintenance Replace the Pressure Sensor Carefully pull out the pressure sensor for about 2 cm. Connect the new pressure sensor to the pressure Then unscrew the cable from the pressure sensor. sensor connector. Fix the pressure sensor to the instrument chassis. Connect the capillary from the pump head outlet to the pressure sensor inlet (1).
Page 161: Replace The Inlet Weaver
Maintenance Replace the Inlet Weaver Replace the Inlet Weaver Parts required Description G4204-81090 Quaternary Pump/Flexible Pump Inlet Weaver Assembly G7131-60090 Inlet Weaver Assembly Bio-Compatible Preparations • Switch off pump at the main power switch • Open the doors • Use an optional solvent shutoff valve or lift up solvent filters inside solvent bottles for avoiding leakages •...
Page 162 Maintenance Replace the Inlet Weaver Fix the fitting of the new inlet weaver to the MCGV. Reconnect tubings between MCGV and degasser. 1260 Infinity II Flexible Pump User Manual...
Page 163: Replace The Inlet Valve (G7104C)
Maintenance Replace the Inlet Valve (G7104C) Replace the Inlet Valve (G7104C) When If Inlet valve is defective. Tools required Description Wrench, 14 mm 5067-5688 Torque wrench 1 – 25 Nm with 14 mm wrench Parts required Description G4204-60022 Inlet Valve Quaternary Pump/Flexible Pump Preparations •...
Page 164: Replace The Inlet Valve (G7131C)
Maintenance Replace the Inlet Valve (G7131C) Replace the Inlet Valve (G7131C) When If Inlet valve is defective. Tools required Description Wrench, 14 mm 5067-5688 Torque wrench 1 – 25 Nm with 14 mm wrench Parts required Description G7131-60022 Inlet Valve Bio-Compatible Flexible Pump Preparations •...
Page 165: Remove The Jet Weaver
Maintenance Remove the Jet Weaver Remove the Jet Weaver Tools required Description 8710-0510 Open-end wrench 1/4 — 5/16 inch 8710-0899 Screwdriver Pozidrive Shaft 5023-2502 Hex driver SW-6.35, slitted Parts required Description 0100-1259 Plastic fittings G4204-04002 Cover RFID Tag Metal lid for Jet Weaver Preparations •...
Page 166 Maintenance Remove the Jet Weaver If no other Jet Weaver shall be installed, use plastic Fix the metal lid to the instrument chassis with a screw. fittings for closing unused ports of the valve and install the metal lid. Otherwise continue at “Install the Jet Weaver”...
Page 167: Install The Jet Weaver
Maintenance Install the Jet Weaver Install the Jet Weaver When Optional Jet Weavers are available for applications which require highest mixing performance, see chapter Optimizing Performance. Tools required Description 8710-0510 Open-end wrench 1/4 — 5/16 inch 8710-0899 Screwdriver Pozidrive Shaft 5023-2502 Hex driver SW-6.35, slitted Parts required...
Page 168 Maintenance Install the Jet Weaver Insert the Jet Weaver to the opening in the front panel Fix the Jet Weaver with a screw. (1) and push it down (2). Mount both capillary connections to the Jet Weaver Connect the inlet capillary of the Jet Weaver to port 2 of observing the correct orientation (long fitting to Jet the Multipurpose Valve.
Page 169: Replace The Seal Wash Pump Cartridge
Maintenance Replace the Seal Wash Pump Cartridge Replace the Seal Wash Pump Cartridge When In case of seal wash pump wear Parts required Description 5065-4445 Peristaltic pump with PharMed tubing 5065-9978 Tubing, 1 mm i.d., 3 mm o.d., silicone, 5 m Preparations Remove the flow connections from and to the seal wash pump.
Page 170: Replace The Multi-Channel Gradient Valve (Mcgv)
Maintenance Replace the Multi-Channel Gradient Valve (MCGV) Replace the Multi-Channel Gradient Valve (MCGV) Tools required Description 0100-1710 Mounting Tool for Tubing Connections 8710-0899 Screwdriver Pozidrive Shaft Parts required Description G1311-67701 Multi channel gradient valve (MCGV) Preparations • Switch off pump at the main power switch •...
Page 171 Maintenance Replace the Multi-Channel Gradient Valve (MCGV) Remove the cover from the MCGV. Disconnect the MCGV cable (1), unscrew the two screws (2) and remove the valve (3). Place the new MCGV into position. Tighten the two screws (1) and connect the cable to its connector (2).
Page 172: Replace The Four-Channel Gradient Valve (4Cgv)
Maintenance Replace the Four-Channel Gradient Valve (4CGV) Replace the Four-Channel Gradient Valve (4CGV) Tools required Description 0100-1710 Mounting Tool for Tubing Connections 8710-0899 Screwdriver Pozidrive Shaft Parts required Description G7111-67701 Four-Channel Gradient Valve (4CGV) Preparations • Switch off pump at the main power switch •...
Page 173 Maintenance Replace the Four-Channel Gradient Valve (4CGV) Place the new 4CGV into position. Tighten the two screws (1) and connect the cable to its connector (2). NOTE Make sure that the THIS SIDE UP label arrow is pointing upward towards the top of the module. Next Steps: Install the 4CGV cover.
Page 174: Release A Stuck Inlet Valve
Maintenance Release a Stuck Inlet Valve Release a Stuck Inlet Valve Tools required Description 9301-0411 Syringe, Plastic 9301-1337 Syringe adapter 0100-1710 Mounting Tool for Tubing Connections Beaker Pressure damages the multi-channel gradient valve (MCGV) and/or degasser C AU T I O N Never apply pressure to the MCGV or degasser.
Page 175 Maintenance Release a Stuck Inlet Valve Slightly open the black plastic screw at the bottom of Disconnect the capillary from the pressure sensor inlet the inlet valve, and rotate the inlet weaver to the front. and route the capillary to a small beaker. Then retighten the screw.
Page 176: Remove The Pump Head Assembly (G7104C)
Maintenance Remove the Pump Head Assembly (G7104C) Remove the Pump Head Assembly (G7104C) Tools required Description G7120-68708 HPLC System Tool Kit-Infinity-II In Lab Advisor go to Service & Diagnostics > Open the doors. Remove/Install Pump Head and follow instructions given on the screen.
Page 177 Maintenance Remove the Pump Head Assembly (G7104C) Turn the inlet valve 90 ° counterclockwise (1) and Loosen the inlet valve. Keep the inlet valve installed to remove the inlet weaver from the inlet valve (2). the pump head assembly. Counter the lock screw of the heat exchanger capillary 10 Remove the four screws holding the pump heads.
Page 178: Remove The Pump Head Assembly (G7131C)
Maintenance Remove the Pump Head Assembly (G7131C) Remove the Pump Head Assembly (G7131C) Tools required Description G7120-68708 HPLC System Tool Kit-Infinity-II In Lab Advisor go to Service & Diagnostics > Open the doors. Remove/Install Pump Head and follow instructions given on the screen.
Page 179 Maintenance Remove the Pump Head Assembly (G7131C) Remove the capillary connection from the outlet Open the black plastic screw of the inlet valve at the adapter on the secondary pump head to the pressure bottom of the primary pump head. sensor.
Page 180 Maintenance Remove the Pump Head Assembly (G7131C) 11 Remove the complete pump head assembly by holding 12 Remove the seal wash tubing interconnecting the two both heads and pulling it to the front. pump heads. 1260 Infinity II Flexible Pump User Manual...
Page 181: Pump Head Maintenance (Tool Free)
Maintenance Pump Head Maintenance (Tool Free) Pump Head Maintenance (Tool Free) Infinity II Flexible Pumps (G7104A/C and G7131A/C) and 1290 Infinity II High-Speed Pumps (G7120A and G7132A) are equipped with Long Life Pump Heads. Long Life Pump Heads offer a significantly increased lifetime of pistons and seals compared to other pump heads.
Page 182: Disassemble Pump Heads (G7104C)
Maintenance Pump Head Maintenance (Tool Free) Disassemble Pump Heads (G7104C) This procedure shows how to open the pump head assembly, exchange seals, and clean pistons. Exchanging seals and cleaning pistons is exemplarily shown for the primary pump head, but works in the same way for the secondary pump head. Tools required Description G7120-68708...
Page 183 Maintenance Pump Head Maintenance (Tool Free) Turn the pump head assembly upside down. Remove the link plate by gently pulling it off the pump head assembly. The two pump chambers are now isolated. Remove the inlet valve and the outlet valve from the Binary/High-Speed Pumps only: Remove the high primary pump head.
Page 184 Maintenance Pump Head Maintenance (Tool Free) Open the pump heads and remove the piston housings 10 Remove the piston by pressing it out of the seal holder from the pump chambers. with a finger. 11 Remove the seal holder from the spring housing. 12 Screw the pin of the Seal Handling Device into the piston seal.
Page 185 Maintenance Pump Head Maintenance (Tool Free) 15 Screw the pin of the Seal Handling Device into the wash 16 Pull out the Seal Handling Device with the wash seal in a seal. straight movement with only gentle force. NOTE The seal holder has two different sides. The black backup ring is supporting the piston seal and must not be removed.
Page 186: Disassemble Pump Heads (G7131C)
Maintenance Pump Head Maintenance (Tool Free) Disassemble Pump Heads (G7131C) This procedure shows how to open the pump head assembly, exchange seals, and clean pistons. Exchanging seals and cleaning pistons is exemplarily shown for the primary pump head, but works in the same way for the secondary pump head. Tools required Description G7120-68708...
Page 187 Maintenance Pump Head Maintenance (Tool Free) Turn the pump head assembly upside down. Remove the link plate by gently pulling it off the pump head assembly. The two pump chambers are now isolated. Remove the inlet valve and the outlet valve from the Binary/High-Speed Pumps only: Remove the primary pump head.
Page 188 Maintenance Pump Head Maintenance (Tool Free) Open the pump heads and remove the piston housings 10 Remove the piston by pressing it out of the seal holder from the pump chambers. with a finger. 11 Remove the seal holder from the spring housing. 12 Screw the pin of the Seal Handling Device into the piston seal.
Page 189 Maintenance Pump Head Maintenance (Tool Free) 15 Screw the pin of the Seal Handling Device into the wash 16 Pull out the Seal Handling Device with the wash seal in a seal. straight movement with only gentle force. NOTE The seal holder has two different sides. The black backup ring is supporting the piston seal and must not be removed.
Page 190: Replace The Heat Exchanger (G7104C)
Maintenance Pump Head Maintenance (Tool Free) Replace the Heat Exchanger (G7104C) Tools required Description Wrench, 19 mm 5023-2501 Screwdriver Torx-T10 5067-5688 Torque wrench 1 – 25 Nm with 14 mm wrench G4220-20013 4 mm hex bit G4220-20015 Adapter ¼ in square to hex G4220-20041 Bit Torx 10x25 mm Parts required...
Page 191 Maintenance Pump Head Maintenance (Tool Free) Remove the screw at the bottom of the pump head. Do Lift out the heat exchanger. not drop the golden spacer fitting. Screw If removed, first insert the spacer fitting. Then insert the Use the 19 mm screw for fixing the front plate. new heat exchanger to the opening in the pump head and lift it over the pins.
Page 192: Replace The Heat Exchanger (G7131C)
Maintenance Pump Head Maintenance (Tool Free) Replace the Heat Exchanger (G7131C) For 1290 Infinity II Bio LC modules, use bio / bio-compatible parts only. Do not mix parts between 1260 Infinity II Bio-Inert LC modules and 1290 Infinity II Bio LC modules. Tools required Description Wrench, 19 mm...
Page 193 Maintenance Pump Head Maintenance (Tool Free) Remove the screw at the bottom of the pump head. Lift out the heat exchanger. Screw Insert the new heat exchanger into the opening of the Use the 19 mm screw for fixing the front plate. pump head and lift it over the pins.
Page 194: Assemble Pump Heads (G7104C)
Maintenance Pump Head Maintenance (Tool Free) Assemble Pump Heads (G7104C) This procedure shows how to exchange seals, and reassemble the pump head assembly. Exchanging seals is exemplarily shown for the primary pump head, but works in the same way for the secondary pump head. Tools required Description G7120-68708...
Page 195 Maintenance Pump Head Maintenance (Tool Free) Take care that the Seal Handling Device is seating flush Repeat for the other pump chamber. and press the seal into the pump chamber. Place the seal holder onto the pump chamber. Place the wash seal onto the designated nose of the Seal Handling Device.
Page 196 Maintenance Pump Head Maintenance (Tool Free) Take care that the Seal Handling Device is seating flush Repeat for the other seal holder. and press the wash seal into the seal holder. NOTE The Seal Handling Device has a cavity to fit over the pins of the seal wash tubings.
Page 197 Maintenance Pump Head Maintenance (Tool Free) 11 Place the seal holder onto the spring housing. 12 Place the assembled spring housings on top of the pump chambers. NOTE NOTE Mind the correct orientation: The backup ring must face Both spring housings are identical, there is no risk when upwards and the seal holder must sit correcly.
Page 198 Maintenance Pump Head Maintenance (Tool Free) 15 Tighten the pump head screws with a torque wrench 16 Mount the pump head to the module. Do not fix the set to approx. 5 Nm in a crosswise manner. screws at this stage! approx.
Page 199 Maintenance Pump Head Maintenance (Tool Free) 21 Counter the outlet valve and tighten the lock screw of 22 Attach the seal wash tubing interconnecting the two the heat exchanger capillary with a torque wrench set to pump heads. approx. 3 Nm. approx.
Page 200: Assemble Pump Heads (G7131C)
Maintenance Pump Head Maintenance (Tool Free) Assemble Pump Heads (G7131C) For 1290 Infinity II Bio LC modules, use bio / bio-compatible parts only. Do not mix parts between 1260 Infinity II Bio-Inert LC modules and 1290 Infinity II Bio LC modules. This procedure shows how to exchange seals, and reassemble the pump head assembly.
Page 201 Maintenance Pump Head Maintenance (Tool Free) Lubricate the seals, the seal holder, and the pump Place the piston seal onto the designated nose of the chambers with isopropanol. Seal Handling Device. The metal spring of the piston seal must be visible. Take care that the Seal Handling Device is seating flush Repeat for the other pump chamber.
Page 202 Maintenance Pump Head Maintenance (Tool Free) Take care that the Seal Handling Device is seating flush Repeat for the other seal holder. and press the wash seal into the seal holder. NOTE The Seal Handling Device has a cavity to fit over the pins of the seal wash tubings.
Page 203 Maintenance Pump Head Maintenance (Tool Free) 11 Place the seal holder onto the spring housing. 12 Place the assembled spring housings on top of the pump chambers. NOTE NOTE Mind the correct orientation: The backup ring must face Both spring housings are identical, there is no risk when upwards and the seal holder must sit correctly.
Page 204 Maintenance Pump Head Maintenance (Tool Free) 15 Tighten the pump head screws with a torque wrench 16 Mount the pump head to the module. Do not fix the set to approx. 5 Nm in a crosswise manner. screws at this stage! approx.
Page 205 Maintenance Pump Head Maintenance (Tool Free) 21 Counter the outlet valve and tighten the lock screw of 22 Attach the seal wash tubing interconnecting the two the heat exchanger capillary with a torque wrench set to pump heads. approximately 3 Nm. 23 Insert the screws that later fix the pump head assembly to the module housing.
Page 206: Install The Pump Head Assembly (G7104C)
Maintenance Install the Pump Head Assembly (G7104C) Install the Pump Head Assembly (G7104C) Tools required Description G7120-68708 HPLC System Tool Kit-Infinity-II 5067-5688 Torque wrench 1 – 25 Nm with 14 mm wrench G4220-20013 4 mm hex bit G4220-20015 Adapter ¼ in square to hex Bring the pump drive to the maintenance position using C AU T I O N the Lab Advisor user interface: Go to Service &...
Page 207 Maintenance Install the Pump Head Assembly (G7104C) Insert the inlet weaver to the inlet valve (1). Fix the Connect the capillary from the outlet adapter on the weaver with the plastic screw to the inlet valve (2). secondary pump head to the pressure sensor. Connect the seal wash tubes.
Page 208: Install The Pump Head Assembly (G7131C)
Maintenance Install the Pump Head Assembly (G7131C) Install the Pump Head Assembly (G7131C) Tools required Description G7120-68708 HPLC System Tool Kit-Infinity-II 5067-5688 Torque wrench 1 – 25 Nm with 14 mm wrench G4220-20013 4 mm hex bit G4220-20015 Adapter ¼ in square to hex Bring the pump drive to the maintenance position using C AU T I O N the Lab Advisor user interface: Go to Service &...
Page 209 Maintenance Install the Pump Head Assembly (G7131C) Insert the inlet weaver to the inlet valve (1). Fix the Connect the capillary from the outlet adapter on the weaver with the plastic screw to the inlet valve (2). secondary pump head to the pressure sensor. Connect the seal wash tubes.
Page 210: Replace The Outlet Valve (G7104C)
Maintenance Replace the Outlet Valve (G7104C) Replace the Outlet Valve (G7104C) When If Outlet valve is defective. Tools required Description Wrench, 14 mm 5067-5688 Torque wrench 1 – 25 Nm with 14 mm wrench G4220-20015 Adapter ¼ in square to hex G4220-20041 Bit Torx 10x25 mm Parts required...
Page 211 Maintenance Replace the Outlet Valve (G7104C) Remove the heat exchanger capillary by pushing the Unscrew the outlet valve with a 14 mm wrench (1) and connector up and pulling it out of the valve. remove it (2). NOTE A gold seal between outlet valve and heat exchanger capillary is used for a tight connection.
Page 212 Maintenance Replace the Outlet Valve (G7104C) Counter the outlet valve and tighten the lock screw of Next Steps: the heat exchanger capillary with a torque wrench set to approx. 3 Nm. 10 Place the cap on the Outlet Valve. approx. 11 Install the pump head assembly, reconnect all hydraulic 3 Nm connections, and power up the pump.
Page 213: Replace The Outlet Valve (G7131C)
Maintenance Replace the Outlet Valve (G7131C) Replace the Outlet Valve (G7131C) When If Outlet valve is defective. Tools required Description 8710-0510 Open-end wrench 1/4 — 5/16 inch Spanner-double open ended 14 mm Parts required Description G7131-60028 Outlet Valve Bio-Compatible Preparations •...
Page 214 Maintenance Replace the Outlet Valve (G7131C) Remove the heat exchanger capillary from the outlet Unscrew the outlet valve with a 14 mm wrench (1) and valve. remove it (2). NOTE A gold seal between outlet valve and heat exchanger capillary is used for a tight connection. Insert the outlet valve into the pump head (1).
Page 215: Replace The Multipurpose Valve
Maintenance Replace the Multipurpose Valve Replace the Multipurpose Valve Tools required Description 5023-2502 Hex driver SW-6.35, slitted Parts required Description 0100-1259 Blank nut (plastic) 01080-83202 Blank nut (stainless steel) 5067-4237 Multipurpose Valve Head 5500-1253 Capillary ST 0.17 mm x 130 mm SX/S (OPTIONAL) 5067-6704 Multipurpose Valve Bio-Compatible 5043-0277...
Page 216 Maintenance Replace the Multipurpose Valve 5 6 7 The central (C) port is connected to the outlet of the filter outlet. • Port 1 is connected to the outlet of the optional Jet Weaver • Port 2 is connected to the inlet of the optional Jet Weaver •...
Page 217: Replace Parts Of The Multipurpose Valve (G7104C)
Maintenance Replace Parts of the Multipurpose Valve (G7104C) Replace Parts of the Multipurpose Valve (G7104C) Tools required Description 8710-2394 9/64 inch hex key Parts required Description 1535-4045 Bearing ring 5068-0202 Rotor seal, Multipurpose Valve, PEEK, 1300 bar 5068-0120 Stator ring 5068-0001 Stator head 1535-4857...
Page 218: Replace Parts Of The Multipurpose Valve (G7131C)
Maintenance Replace Parts of the Multipurpose Valve (G7131C) Replace Parts of the Multipurpose Valve (G7131C) Tools required Description 8710-2394 9/64 inch hex key Parts required Description 5068-0379 Rotor Seal Bio-Compatible, Multipurpose Valve Bio-Compatible, PEEK, 1300 bar 5068-0378 Stator head Bio-Compatible 5428-0006 Stator screws Bio-Compatible Preparations...
Page 219: Replace The High Pressure Outlet Filter Or Filter Frit
Maintenance Replace the High Pressure Outlet Filter or Filter Frit Replace the High Pressure Outlet Filter or Filter Frit When For removing blockages and leaks in the high pressure filter assembly. The outlet filter should be replaced as required depending on the system usage. Tools required Description 5023-2502...
Page 220 Maintenance Replace the High Pressure Outlet Filter or Filter Frit Unscrew the high pressure outlet filter. Replace the filter frit. NOTE Remove the old filter frit with the sharp side of the blade of a knife. Frit Reassemble the high pressure outlet filter (approx. Reinstall the high pressure outlet filter to the filter 16 Nm).
Page 221: Install The Inline Filter
Maintenance Install the Inline Filter Install the Inline Filter Tools required Description 8710-0510 Open-end wrench 1/4 — 5/16 inch Parts required Description G7104-68000 Inline Filter Upgrade Kit G7131-68000 Inline Filter Upgrade Kit Bio-Compatible Preparations Turn the pump off. Remove the capillary between ports 5 and 8 from the Clip the inline filter clamp to the Multipurpose Valve.
Page 222 Maintenance Install the Inline Filter Install the integrated capillary of the inline filter to port 5 Install the removable capillary of the inline filter to port 8 of the Multipurpose Valve. of the Multipurpose Valve. 1260 Infinity II Flexible Pump User Manual...
Page 223: Remove The Inline Filter
Maintenance Remove the Inline Filter Remove the Inline Filter Tools required Description 8710-0510 Open-end wrench 1/4 — 5/16 inch Parts required Description 5067-5416 Capillary ST 0.17 x 120 mm, SLV/SV 5500-1284 Capillary MP35N 0.17 mm x 120 mm SI/SX Remove the capillaries from the Multipurpose Valve to Remove the clamp with the inline filter.
Page 224: Replace Parts Of The Inline Filter
Maintenance Replace Parts of the Inline Filter Replace Parts of the Inline Filter Tools required Description 8710-0510 Open-end wrench 1/4 — 5/16 inch Parts required Description 5023-0271 Frit 0.3 µm for inline filter, 5/pk 5230-0022 Frit 0.3 µm for Inline Filter Bio-Compatible Stuck Capillary in Multi Purpose Valve C AU T I O N Shortcutting the inline filter by directly connecting its right capillary to valve...
Page 225 Maintenance Replace Parts of the Inline Filter Use two 5/16 wrenches for opening the inline filter. Replace the filter frit and reassemble the inline filter. Filter frit Put the inline filter to the clamp and install its capillaries. The integrated capillary is connected to port 5 of the Multi Purpose Valve.
Page 226: Replace The Seal Wash Sensor
Maintenance Replace the Seal Wash Sensor Replace the Seal Wash Sensor Parts required Description 5067-6172 Seal Wash Sensor Pre Assembly Preparations • Shut-down the pump. • Remove the seal wash tubing from the seal wash solvent bottle. Remove the solvent inlet and outlet tubes from the seal Press at the sides of the seal wash sensor.
Page 227 Maintenance Replace the Seal Wash Sensor Remove the seal wash sensor completely. Connect the cable to the new seal wash sensor. Install the new sensor to the module chassis. Install the solvent inlet and outlet tubes to the seal wash sensor.
Page 228: Replace The Module Firmware
Maintenance Replace the Module Firmware Replace the Module Firmware When The installation of newer firmware might be necessary • if a newer version solves problems of older versions or • to keep all systems on the same (validated) revision. The installation of older firmware might be necessary •...
Page 229: Prepare The Pump Module For Transport
Maintenance Prepare the Pump Module for Transport Prepare the Pump Module for Transport When If the module shall be transported or shipped. Parts required Description 9301-0411 Syringe; Plastic 9301-1337 Syringe adapter G7104-44000 Transport protection foam (G7104A/C) G7131-98000 Transport protection foam (G7131A/C) Mechanical damage C AU T I O N For shipping the module, insert the Protective Foam to protect the module...
Page 230 Maintenance Prepare the Pump Module for Transport Reconnect the degasser outlet tubings to the MCGV. 10 You may keep internal tubing and capillary connections. Remove the degasser inlet tubings. MCGV Pressure sensor Degasser High pressure outlet filter Valve Inlet weaver Inline filter Pump heads Next Steps:...
Page 231: Parts And Materials For Maintenance
Parts and Materials for Maintenance Overview of Main Assemblies (G7104C) Overview of Main Assemblies (G7131C) Flow Connections (G7104C) Flow Connections (G7131C) Pump Heads (G7104C) Pump Head Assembly Parts Primary Pump Head Parts Secondary Pump Head Parts Pump Heads (G7131C) Pump Head Assembly Parts Primary Pump Head Parts Secondary Pump Head Parts Multipurpose Valve (G7104C)
Page 232: Overview Of Main Assemblies (G7104C)
Parts and Materials for Maintenance Overview of Main Assemblies (G7104C) Overview of Main Assemblies (G7104C) 1260 Infinity II Flexible Pump User Manual...
Page 233 Parts and Materials for Maintenance Overview of Main Assemblies (G7104C) Item Description 5067-5892 Status-LED Assembly 700 5067-6172 Seal Wash Sensor Pre Assembly G4204-60004 Outlet filter Quaternary Pump/Flexible Pump G4204-68000 Jet Weaver 380 µL for Quaternary Pump/Flexible Pump (not shown) (OPTIONAL) 5067-4237 Multipurpose Valve Head 5067-5407...
Page 234: Overview Of Main Assemblies (G7131C)
Parts and Materials for Maintenance Overview of Main Assemblies (G7131C) Overview of Main Assemblies (G7131C) 1260 Infinity II Flexible Pump User Manual...
Page 235 Parts and Materials for Maintenance Overview of Main Assemblies (G7131C) Item Description 5067-5892 Status-LED Assembly 700 5067-6172 Seal Wash Sensor Pre Assembly G7131-60004 Outlet Filter Flex Bio-Compatible 5067-6704 Multipurpose Valve Bio-Compatible 5720-0003 Inline Filter Assembly, Bio-Compatible 5067-7010 Pressure sensor Bio-Compatible 1300 bar G7131-60300 Bio-Compatible Pump Head Flexible G7131-60090...
Page 236: Flow Connections (G7104C)
Parts and Materials for Maintenance Flow Connections (G7104C) Flow Connections (G7104C) Figure 21 Flow connections of the pump 1260 Infinity II Flexible Pump User Manual...
Page 237 Parts and Materials for Maintenance Flow Connections (G7104C) Item Description G4220-60035 Tubing kit 140 mm, 2/pk Degasser to MCGV G7120-60007 Bottle Head Assembly 5067-6871 Solvent Cabinet Kit (not shown) 5065-9978 Tubing, 1 mm i.d., 3 mm o.d., silicone, 5 m 5065-4445 Peristaltic pump with PharMed tubing 5067-4656...
Page 238: Flow Connections (G7131C)
Parts and Materials for Maintenance Flow Connections (G7131C) Flow Connections (G7131C) Figure 22 Flow connections of the pump 1260 Infinity II Flexible Pump User Manual...
Page 239 Parts and Materials for Maintenance Flow Connections (G7131C) Item Description G4220-60035 Tubing kit 140 mm, 2/pk Degasser to 4CGV G7120-60007 Bottle Head Assembly 5067-6871 Solvent Cabinet Kit (not shown) 5065-9978 Tubing, 1 mm i.d., 3 mm o.d., silicone, 5 m 5065-4445 Peristaltic pump with PharMed tubing 5500-1283...
Page 240: Pump Heads (G7104C)
Parts and Materials for Maintenance Pump Heads (G7104C) Pump Heads (G7104C) The following pages contain parts information for LongLife Pump Heads. For parts information on other pump head types, please refer to Agilent 1290 Infinity II Easy Maintenance Pump Head Technical Note (01200-90120) and to Agilent 1290 Infinity Pump Head Maintenance Technical Note (G4220-90122).
Page 241: Pump Head Assembly Parts
Parts and Materials for Maintenance Pump Heads (G7104C) Pump Head Assembly Parts Figure 23 Pump head assembly parts Long Life Pump Head Quat (G4204-60350) Item Description G4220-60660 Secondary Pump Head Assembly Pendulum G4220-60661 Primary Pump Head Assembly Pendulum G4204-60022 Inlet Valve Quaternary Pump/Flexible Pump G4220-60028 Outlet valve (primary pump head)
Page 242: Primary Pump Head Parts
Parts and Materials for Maintenance Pump Heads (G7104C) Primary Pump Head Parts Primary Pump Head Assembly Pendulum (G4220-60661) Item Description 5067-5975 Plunger Assy ZrO 5810-0009 Screw M5x40 A4-80 Lubric Silver G4220-60046 Preload-Support Assembly LL 0905-1175 Wash seal (PTFE) G4220-60616 Seal Holder Integrated Assembly EM/LL 0905-1719 PE Seal G4220-60533...
Page 243: Secondary Pump Head Parts
Parts and Materials for Maintenance Pump Heads (G7104C) Secondary Pump Head Parts Secondary Pump Head Assembly Pendulum (G4220-60660) Item Description 5067-5975 Plunger Assy ZrO 5810-0009 Screw M5x40 A4-80 Lubric Silver G4220-60046 Preload-Support Assembly LL 0905-1175 Wash seal (PTFE) G4220-60616 Seal Holder Integrated Assembly EM/LL 0905-1719 PE Seal G4220-25513...
Page 244: Pump Heads (G7131C)
Parts and Materials for Maintenance Pump Heads (G7131C) Pump Heads (G7131C) Pump Head Assembly Parts Figure 24 Pump head assembly parts Bio-Compatible Pump Head Flexible (G7131-60300) Item Description G7131-60640 Secondary Pump Head Assembly Bio-Compatible G7131-60641 Primary Pump Head Assembly Bio-Compatible G7131-60022 Inlet Valve Bio-Compatible Flexible Pump G7131-60028...
Page 245: Primary Pump Head Parts
Parts and Materials for Maintenance Pump Heads (G7131C) Primary Pump Head Parts Primary Pump Head Assembly Bio-Compatible (G7131-60641) Item Description 5067-5975 Plunger Assy ZrO 5810-0009 Screw M5x40 A4-80 Lubric Silver G4220-60046 Preload-Support Assembly LL 0905-1731 Bio-Inert Wash Seal G7131-60616 Seal Holder Integrated Assembly Bio-Compatible G7131-20009 Seal Bio-Compatible G7131-60533...
Page 246: Secondary Pump Head Parts
Parts and Materials for Maintenance Pump Heads (G7131C) Secondary Pump Head Parts Secondary Pump Head Assembly Bio-Compatible (G7131-60640) Item Description 5067-5975 Plunger Assy ZrO 5810-0009 Screw M5x40 A4-80 Lubric Silver G4220-60046 Preload-Support Assembly LL 0905-1731 Bio-Inert Wash Seal G7131-60616 Seal Holder Integrated Assembly Bio-Compatible G7131-20009 Seal Bio-Compatible G7132-60000...
Page 247: Multipurpose Valve (G7104C)
Parts and Materials for Maintenance Multipurpose Valve (G7104C) Multipurpose Valve (G7104C) Item Description 5067-4237 Multipurpose Valve Head 1535-4857 Stator screws, 10/pk 5068-0001 Stator head 5068-0120 Stator ring 5068-0202 Rotor seal, Multipurpose Valve, PEEK, 1300 bar 1535-4045 Bearing ring 5068-0106 Spanner nut 5067-4106 Valve drive Valve drive housing...
Page 248: Multipurpose Valve (G7131C)
Parts and Materials for Maintenance Multipurpose Valve (G7131C) Multipurpose Valve (G7131C) Item Description 5067-6704 Multipurpose Valve Bio-Compatible 5428-0006 Stator screws Bio-Compatible 5068-0378 Stator head Bio-Compatible 5068-0379 Rotor Seal Bio-Compatible, Multipurpose Valve Bio-Compatible, PEEK, 1300 bar 5068-0106 Spanner nut 5067-4106 Valve drive G4204-40001 Valve Drive Holder 2200-0141...
Page 249: Cover Parts
Parts and Materials for Maintenance Cover Parts Cover Parts Top cover Side cover left Door assy right Door assy left Base cover Side cover right Description G7104-68713 Cabinet Kit 180 Infinity II (includes sides, bottom, top, leak adapter top and Status Indicator Insert) 5043-0286 Base Cover Infinity II...
Page 250: Accessory Kit (G7104C)
Parts and Materials for Maintenance Accessory Kit (G7104C) Accessory Kit (G7104C) G7104-68705 Description G4220-67000 Waste Tubing with Fitting 5043-1013 Tubing Clip 5063-6527 Tubing assembly, i.d. 6 mm, o.d. 9 mm, 1.2 m (to waste) 5067-5443 Inlet tubing 5181-1519 CAN cable, Agilent module to module, 1 m 5500-1155 Tube Connector, 90 degree, ID 6.4 5500-1246...
Page 251: Accessory Kit (G7131C)
Parts and Materials for Maintenance Accessory Kit (G7131C) Accessory Kit (G7131C) G7131-68755 Description 5043-1013 Tubing Clip 5063-6527 Tubing assembly, i.d. 6 mm, o.d. 9 mm, 1.2 m (to waste) 5067-5443 Inlet tubing 5181-1519 CAN cable, Agilent module to module, 1 m 5500-1155 Tube Connector, 90 degree, ID 6.4 5500-1419...
Page 252: Tool Kit
Parts and Materials for Maintenance Tool Kit Tool Kit 5023-2504 (Hex Driver SW-4 slitted) 5023-2503 8710-0899 (Hex Driver (Screwdriver Pozidrive Shaft) SW-5 slitted) 5023-2502 (Hex Driver SW-6, 35/ ¼” slitted) 5023-3088 8710-1924 8710-2409 8710-0510 8710-0510 5023-2500 8710-1534 8710-2394 (Hex Key 9/64“, 15cm) Box with: 9301-0411 (Syringe, Plastic) 5023-3089...
Page 253: Identifying Cables
Identifying Cables Cable Overview Analog Cables Remote Cables CAN/LAN Cables Agilent Module to PC USB Cables This chapter provides information on cables used with the modules. 1260 Infinity II Flexible Pump User Manual...
Page 254: Cable Overview
Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure NOTE proper functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent 35900A A/D converter 01046-60105 Analog cable (BNC to general purpose, spade lugs)
Page 255 Identifying Cables Cable Overview LAN cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection) RS-232 cables (not for Description FUSION board) RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female).
Page 256: Analog Cables
Identifying Cables Analog Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules. The other end depends on the instrument to which connection is being made. Agilent Module to 35900 A/D converters p/n 35900-60750 35900 Pin Agilent...
Page 257 Identifying Cables Analog Cables Agilent Module to BNC Connector p/n 8120-1840 Pin BNC Pin Agilent Signal Name module Shield Shield Analog - Center Center Analog + Agilent Module to General Purpose p/n 01046-60105 Pin Agilent Signal Name module Not connected Black Analog - Analog +...
Page 258: Remote Cables
Identifying Cables Remote Cables Remote Cables ERI (Enhanced Remote Interface) • 5188-8029 ERI to general purpose (D-Sub 15 pin male - open end) • 5188-8044 ERI to ERI (D_Sub 15 pin male - male) • 5188-8059 ERI-Extension-Cable 1.2 m (D-Sub15 pin male / female) p/n 5188-8029 Color code Enhanced...
Page 259 Identifying Cables Remote Cables • 5188-8045 ERI to APG (Connector D_Subminiature 15 pin (ERI), Connector D_Subminiature 9 pin (APG)) p/n 5188-8045 Pin (ERI) Signal Pin (APG) Active (TTL) Start Request Stop Ready High Power on High Future Shut Down Start Prepare Ground Cable Shielding...
Page 260 Ground Cable Shielding One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. 1260 Infinity II Flexible Pump User Manual...
Page 261 Identifying Cables Remote Cables Agilent Module to Agilent 35900 A/D Converters p/n 5061-3378 Pin 35900 A/D Pin Agilent Signal Name Active module (TTL) 1 - White 1 - White Digital ground 2 - Brown 2 - Brown Prepare run 3 - Gray 3 - Gray Start 4 - Blue...
Page 262: Can/Lan Cables
Identifying Cables CAN/LAN Cables CAN/LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors. CAN Cables Description 5181-1516 CAN cable, Agilent module to module, 0.5 m 5181-1519 CAN cable, Agilent module to module, 1 m LAN Cables Description 5023-0203...
Page 263: Agilent Module To Pc
Identifying Cables Agilent Module to PC Agilent Module to PC Description RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It is also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.
Page 264: Usb Cables
Identifying Cables USB Cables USB Cables To connect a USB Flash Drive use a USB OTG cable with Mini-B plug and A socket. Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) 1260 Infinity II Flexible Pump User Manual...
Page 265: Hardware Information
Hardware Information Firmware Description Electrical Connections Serial Number Information Rear View of the Module Interfaces Overview Interfaces USB (Universal Serial Bus) Setting the 8-bit Configuration Switch Special Settings Early Maintenance Feedback Instrument Layout This chapter describes the pump in more detail on hardware and electronics. 1260 Infinity II Flexible Pump User Manual...
Page 266: Firmware Description
Hardware Information Firmware Description Firmware Description The firmware of the instrument consists of two independent sections: • a non-instrument specific section, called resident system • an instrument specific section, called main system Resident System This resident section of the firmware is identical for all Agilent 1100/1200/1220/1260/1290 series modules.
Page 267 Hardware Information Firmware Description Firmware Updates Firmware updates can be done with the Agilent Lab Advisor software with files on the hard disk (latest version should be used). Required tools, firmware and documentation are available from the Agilent web: http://www.agilent.com/en-us/firmwareDownload?whid=69761 The file naming conventions are: PPPP_RVVV_XXX.dlb, where •...
Page 268 Hardware Information Firmware Description Some modules are limited in downgrading due to their main board version or NOTE their initial firmware revision. For example, a G1315C DAD SL cannot be downgraded below firmware revision B.01.02 or to a A.xx.xx. Some modules can be re-branded (e.g. G1314C to G1314B) to allow operation in specific control software environments.
Page 269: Electrical Connections
There are no externally accessible fuses, because automatic electronic fuses are implemented in the power supply. Never use cables other than the ones supplied by Agilent Technologies to ensure NOTE proper functionality and compliance with safety or EMC regulations.
Page 270: Serial Number Information
Hardware Information Electrical Connections Serial Number Information The serial number information on the instrument labels provide the following information: CCXZZ00000 Format Country of manufacturing • DE = Germany • JP = Japan • CN = China Alphabetic character A-Z (used by manufacturing) Alpha-numeric code 0-9, A-Z, where each combination unambiguously denotes a module (there can be more than one code for the same module)
Page 271: Interfaces
Hardware Information Interfaces Interfaces The Agilent InfinityLab LC Series modules provide the following interfaces: Table 18 Agilent InfinityLab LC Series Interfaces Module RS-232 Analog APG (A) Special (on-board) / ERI (E) Pumps G7104A/C G7110B G7111A/B, G5654A G7112B G7120A G7161A/B Samplers...
Page 272 Hardware Information Interfaces Table 18 Agilent InfinityLab LC Series Interfaces Module RS-232 Analog APG (A) Special (on-board) / ERI (E) Fraction Collectors G7159B G7166A Requires a host module with on-board LAN with minimum FW B.06.40 or C.06.40, or with additional G1369C LAN...
Page 273: Overview Interfaces
Hardware Information Interfaces Overview Interfaces The CAN is inter-module communication interface. It is a 2-wire serial bus system supporting high speed data communication and real-time requirement. The modules have either an interface slot for a LAN card (e.g. Agilent G1369B/C LAN Interface) or they have an on-board LAN interface (e.g.
Page 274 Remote (ERI) The ERI (Enhanced Remote Interface) connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. It allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
Page 275: Usb (Universal Serial Bus)
Hardware Information Interfaces Table 19 ERI signal distribution Signal Description START REQUEST (L) Request to start injection cycle (for example, by start key on any module). Receiver is the autosampler. STOP (L) Request to reach system ready state as soon as possible (for example, stop run, abort or finish and stop injection).
Page 276: Setting The 8-Bit Configuration Switch
Hardware Information Setting the 8-bit Configuration Switch Setting the 8-bit Configuration Switch The 8-bit configuration switch is located at the rear of the module. Switch settings provide configuration parameters for LAN, serial communication protocol and instrument specific initialization procedures. All modules with on-board LAN: •...
Page 277 Hardware Information Setting the 8-bit Configuration Switch To perform any LAN configuration, SW1 and SW2 must be set to OFF. For details NOTE on the LAN settings/configuration refer to chapter LAN Configuration. Table 20 8-bit Configuration Switch (with on-board LAN) Mode Function SW 1...
Page 278: Special Settings
Hardware Information Setting the 8-bit Configuration Switch Special Settings The special settings are required for specific actions (normally in a service case). The tables include both settings for modules – with on-board LAN and without NOTE on-board LAN. They are identified as LAN and no LAN. Boot-Resident Firmware update procedures may require this mode in case of firmware loading errors (main firmware part).
Page 279: Early Maintenance Feedback
Hardware Information Early Maintenance Feedback Early Maintenance Feedback Maintenance requires the exchange of components that are subject to wear or stress. Ideally, the frequency at which components are exchanged should be based on the intensity of use of the module and the analytical conditions, and not on a predefined time interval.
Page 280: Instrument Layout
Hardware Information Instrument Layout Instrument Layout The industrial design of the module incorporates several innovative features. It uses Agilent’s E-PAC concept for the packaging of electronics and mechanical assemblies. This concept is based upon the use of expanded polypropylene (EPP) layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed.
Page 281 LAN Configuration What You Have To Do First TCP/IP Parameter Configuration Configuration Switch Initialization Mode Selection Dynamic Host Configuration Protocol (DHCP) General Information (DHCP) Setup (DHCP) Link Configuration Selection Manual Configuration With Telnet PC and User Interface Software Setup PC Setup for Local Configuration User Interface Software Setup This chapter provides information on connecting the module to the controller software.
Page 282: Lan Configuration What You Have To Do First
LAN Configuration What You Have To Do First What You Have To Do First The module has an on-board LAN communication interface. 1 Note the MAC (Media Access Control) address for further reference. The MAC or hardware address of the LAN interfaces is a world wide unique identifier. No other network device will have the same hardware address.
Page 283: Tcp/Ip Parameter Configuration
LAN Configuration TCP/IP Parameter Configuration TCP/IP Parameter Configuration To operate properly in a network environment, the LAN interface must be configured with valid TCP/IP network parameters. These parameters are: • IP address • Subnet Mask • Default Gateway The TCP/IP parameters can be configured by the following methods: •...
Page 284: Configuration Switch
LAN Configuration Configuration Switch Configuration Switch The configuration switch can be accessed at the rear of the module. Configuration switch Figure 30 Location of Configuration Switch The module is shipped with all switches set to OFF, as shown above. To perform any LAN configuration, SW1 and SW2 must be set to OFF. NOTE Table 23 Factory Default Settings...
Page 285: Initialization Mode Selection
LAN Configuration Initialization Mode Selection Initialization Mode Selection The following initialization (init) modes are selectable: Table 24 Initialization Mode Switches SW 6 SW 7 SW 8 Init Mode Using Stored Using Default DHCP Requires firmware B.06.40 or above. Modules without LAN on board, see G1369C LAN Interface Card Using Stored When initialization mode Using Stored is selected, the parameters are taken from the non-volatile memory of the module.
Page 286 LAN Configuration Initialization Mode Selection Using Default When Using Default is selected, the factory default parameters are taken instead. These parameters enable a TCP/IP connection to the LAN interface without further configuration, see Table 25 on page 286. Default Active Parameter Parameter Figure 32...
Page 287: Dynamic Host Configuration Protocol (Dhcp)
LAN Configuration Dynamic Host Configuration Protocol (DHCP) Dynamic Host Configuration Protocol (DHCP) General Information (DHCP) The Dynamic Host Configuration Protocol (DHCP) is an auto configuration protocol used on IP networks. The DHCP functionality is available on all Agilent HPLC modules with on-board LAN Interface or LAN Interface Card G1369C, and “B”-firmware (B.06.40 or above) or modules with "D"-firmware.
Page 288: Setup (Dhcp)
LAN Configuration Dynamic Host Configuration Protocol (DHCP) Setup (DHCP) The DHCP functionality is available on all Agilent HPLC modules with on-board LAN Interface or LAN Interface Card G1369C, and “B”-firmware (B.06.40 or above) or modules with "D"-firmware. All modules should use latest firmware from the same set. 1 Note the MAC address of the LAN interface (provided with G1369C LAN Interface Card or mainboard).
Page 289 LAN Configuration Dynamic Host Configuration Protocol (DHCP) 2 Set the configuration switch to DHCP either on the G1369C LAN Interface Card or the mainboard of above mentioned modules. Table 26 G1369C LAN Interface Card (configuration switch on the card) SW 4 SW 5 SW 6 SW 7...
Page 290: Link Configuration Selection
LAN Configuration Link Configuration Selection Link Configuration Selection The LAN interface supports 10 or 100 Mbps operation in full- or half-duplex modes. In most cases, full-duplex is supported when the connecting network device - such as a network switch or hub - supports IEEE 802.3u auto-negotiation specifications.
Page 291: Manual Configuration
LAN Configuration Manual Configuration Manual Configuration Manual configuration only alters the set of parameters stored in the non-volatile memory of the module. It never affects the currently active parameters. Therefore, manual configuration can be done at any time. A power cycle is mandatory to make the stored parameters become the active parameters, given that the initialization mode selection switches are allowing it.
Page 292: With Telnet
LAN Configuration Manual Configuration With Telnet Whenever a TCP/IP connection to the module is possible (TCP/IP parameters set by any method), the parameters may be altered by opening a Telnet session. 1 Open the system (DOS) prompt window by clicking on Windows START button and select “Run...”.
Page 293 LAN Configuration Manual Configuration 3 Type ? and press enter to see the available commands. Figure 38 Telnet Commands Table 29 Telnet Commands Value Description displays syntax and descriptions of commands displays current LAN settings ip <x.x.x.x> sets new ip address sm <x.x.x.x>...
Page 294 LAN Configuration Manual Configuration 5 Use the “/” and press Enter to list the current settings. information about the LAN interface MAC address, initialization mode Initialization mode is Using Stored active TCP/IP settings TCP/IP status - here ready connected to PC with controller software (e.g. Agilent ChemStation), here not connected Figure 39 Telnet - Current settings in "Using Stored"...
Page 295 LAN Configuration Manual Configuration 7 When you have finished typing the configuration parameters, type exit and press Enter to exit with storing parameters. Figure 41 Closing the Telnet Session If the Initialization Mode Switch is changed now to “Using Stored” mode, the NOTE instrument will take the stored settings when the module is re-booted.
Page 296: Pc And User Interface Software Setup
LAN Configuration PC and User Interface Software Setup PC and User Interface Software Setup PC Setup for Local Configuration This procedure describes the change of the TCP/IP settings on your PC to match the module’s default parameters in a local configuration (see also “Initialization Mode Selection”...
Page 297: User Interface Software Setup
LAN Configuration PC and User Interface Software Setup User Interface Software Setup Install you user interface software according the provided User Interface Software Setup Guide. 1260 Infinity II Flexible Pump User Manual...
Page 298: Appendix
Do Not Modify the Instrument In Case of Damage Solvents Safety Symbols Waste Electrical and Electronic Equipment Directive Radio Interference Sound Emission Agilent Technologies on Internet This chapter provides additional information on safety, legal and web. 1260 Infinity II Flexible Pump User Manual...
Page 299: General Safety Information
Appendix General Safety Information General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument.
Page 300: Before Applying Power
Verify that the voltage range and frequency of your power distribution  matches to the power specification of the individual instrument. Never use cables other than the ones supplied by Agilent Technologies  to ensure proper functionality and compliance with safety or EMC regulations.
Page 301: Do Not Operate In An Explosive Atmosphere
Appendix General Safety Information Do Not Operate in an Explosive Atmosphere Presence of flammable gases or fumes WAR N IN G Explosion hazard Do not operate the instrument in the presence of flammable gases or  fumes. Do Not Remove the Instrument Cover Instrument covers removed WAR N IN G Electrical shock...
Page 302: Solvents
Appendix General Safety Information Solvents Toxic, flammable and hazardous solvents, samples and reagents WAR N IN G The handling of solvents, samples and reagents can hold health and safety risks. When working with these substances observe appropriate safety  procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.
Page 303: Safety Symbols
Appendix General Safety Information Safety Symbols Table 30 Symbols The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage. Indicates dangerous voltages.
Page 304 Appendix General Safety Information Table 30 Symbols Magnetic field Magnets produce a far-reaching, strong magnetic field. They could damage TVs and laptops, computer hard drives, credit and ATM cards, data storage media, mechanical watches, hearing aids and speakers. Keep magnets at least 25 mm away from devices and objects that could be damaged by strong magnetic fields.
Page 305: Waste Electrical And Electronic Equipment Directive
Appendix Waste Electrical and Electronic Equipment Directive Waste Electrical and Electronic Equipment Directive This product complies with the European WEEE Directive marking requirements. The affixed label indicates that you must not discard this electrical/electronic product in domestic household waste. Do not dispose of in domestic household waste NOTE To return unwanted products, contact your local Agilent office, or see http://www.agilent.com...
Page 306: Radio Interference
Appendix Radio Interference Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference. All cables are in compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with unscreened cables, or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises.
Page 307: Sound Emission
Appendix Sound Emission Sound Emission Sound pressure Sound pressure Lp <70 db(A) according to DIN EN ISO 7779 Schalldruckpegel Schalldruckpegel Lp <70 db(A) nach DIN EN ISO 7779 1260 Infinity II Flexible Pump User Manual...
Page 308: Agilent Technologies On Internet
Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com 1260 Infinity II Flexible Pump User Manual...
Page 309 Appendix Agilent Technologies on Internet 1260 Infinity II Flexible Pump User Manual...
Page 310 • using and optimizing, • troubleshooting and diagnostic, • error information, • test functions, • maintenance, • parts identification, • hardware information, • safety and related information. www.agilent.com  Agilent Technologies Inc. 2021 Edition: 6/2021 Document No: SD-29000018 Rev. C...

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Agilent Technologies InfinityLab LC Series User Manual

1 Introduction

Agilent 1260 Infinity II Flexible Pump

Agilent 1260 Infinity II Bio Flexible Pump

Operating Principle

Positions of the Multi Purpose Valve

Leak and Waste Handling

2 Site Requirements and Specifications

3 Using the Module

1260 Infinity II Flexible Pumps 65

4 Preparing the Pump

5 How to Optimize the Performance of Your Module

6 Troubleshooting and Diagnostics

7 Error Information

8 Maintenance

9 Parts and Materials for Maintenance

10 Identifying Cables

11 Hardware Information

12 LAN Configuration What You Have to Do First

13 Appendix

Quick Links

Troubleshooting

Need help?

Questions and answers

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Summary of Contents for Agilent Technologies InfinityLab LC Series