Agilent Technologies 1260 Infinity II User Manual
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Agilent Technologies 1260 Infinity II User Manual

Infinitylab lc series preparative autosampler
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  • Page 1 Agilent InfinityLab LC Series 1260 Infinity II Preparative Autosampler User Manual Agilent Technologies...
  • Page 2 (June 1987) or any equivalent agency regu- lation or contract clause. Use, duplication or disclosure of Software is subject to Agi- lent Technologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 3 Overview about the troubleshooting and diagnostic features. 8 Error Information This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 4 This chapter describes the detector in more detail on hardware and electronics. 13 LAN Configuration This chapter provides information on connecting the module to the Agilent ChemStation PC. 14 Appendix This chapter provides addition information on safety, legal and web. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 5: Table Of Contents

    Transporting the Sampler with a Sample Cooler Installed Agilent Local Control Modules 4 Installation of the Optional Sample Cooler Injector Step Commands Damaged Packaging Install the Sample Cooler Using the Sample Cooler in an Infinity II Sampler Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 6 Step Commands Tests Troubleshooting Troubleshooting Guide for the Sample Transport Assembly Agilent Lab Advisor Software 8 Error Information What are Error Messages General Error Messages Sampler Error Messages Sample Cooler Error Messages Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 7 Standard Parts Drawer Assembly External Tray Analytical-Head Assembly (900 µL) Standard Sampler Accessory Kit Multi-Draw Kit Additional Part List 11 Identifying Cables Cable Overview Analog Cables Remote Cables CAN/LAN Cables RS-232 Cables Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 8 Dynamic Host Configuration Protocol (DHCP) Manual Configuration PC and Agilent ChemStation Setup 14 Appendix General Safety Information Waste Electrical and Electronic Equipment Directive Refrigerant Radio Interference Sound Emission Solvent Information Agilent Technologies on Internet Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 9: Introduction

    Agilent 1260 Infinity II Preparative Autosampler User Manual Introduction Product Description Features Overview of the Module Operating Principle Sampling Sequence Needle Parkstation Hydraulic Box Transport Assembly Leak and Waste Handling Leak Sensor Waste Concept This chapter gives an introduction to the module and instrument overview.

  • Page 10: Product Description

    Introduction Product Description Product Description The 1260 Infinity II Preparative Autosampler is designed for laboratories that need automated sample injection to increase daily throughput of purified sample. This autosampler is easy to use, reliable, and capable of injecting sample volumes from vials in the microliter to milliliter range.

  • Page 11: Features

    • Support of flow rates up to 100 mL/min at 400 bar Laboratory efficiency • Maximum capacity of 132 sample vials within the same foot print • Flexible and convenient sample handling with different types of sample containers Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 12: Overview Of The Module

    Overview of the Module Overview of the Module The Agilent 1260 Infinity II Preparative Autosampler is designed for use with other modules of the Agilent 1200 Infinity Series, 1200 Series, and 1100 Series LC, or with other LC systems if adequate remote control inputs and outputs are available.

  • Page 13: Operating Principle

    If this feature is disabled, the gripper arm positions the sample vial directly below the needle (Step 4) and lowers the needle into the vial. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 14 This ensures that all parts in contact with sample are flushed during the run, thus minimizing carry-over. Figure 2 Mainpass Position - standard position during runs and when the sampler is idle Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 15 15). Solvent from the pump enters the valve unit at port 1, and flows directly to the column through port 6. Figure 3 Valve in bypass - needle in vial, metering device aspirates sample volume Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 16 In the next step, the needle is washed (see Figure 4 page 16). Figure 4 Outer face of needle getting washed in wash port Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 17 (see Figure 5 on page 17). Figure 5 Valve switches to main pass - sample is transferred towards the LC column Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 18: Needle Parkstation

    The upper and lower needle positions are detected by reflection sensors on the needle station board, while the needle-in-vial position is determined by counting the motor steps from the upper needle-sensor position. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 19: Hydraulic Box

    The backward movement of the piston (driven by the spring) draws sample from the vial. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 20 Two microswitches monitor switching of the valve (bypass and mainpass end positions). No valve adjustments are required after replacing internal components. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 21: Transport Assembly

    These positions are used by the processor to calculate the actual motor position. An additional six reflection sensors for tray recognition are mounted on the flex board at the front of the assembly. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 22: Leak And Waste Handling

    PVDF (polyvinylidene fluoride), is incompatible with DMF. ➔ Do not use DMF. The leak sensor in the sampler is hidden under the ICC Column Heater or Column Shelf N O T E respectively. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 23: Waste Concept

    1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with 4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don't immerse in the liquid. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 24 Introduction Leak and Waste Handling Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 25: Site Requirements And Specifications

    Agilent 1260 Infinity II Preparative Autosampler User Manual Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications Specifications of the Sample Cooler This chapter provides information on environmental requirements, physical and performance specifications. Agilent Technologies...

  • Page 26: Site Requirements

    ➔ Make sure the power connector of the instrument can be easily reached and unplugged. ➔ Provide sufficient space behind the power socket of the instrument to unplug the cable. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 27 Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. Power cords WA R N I N G Solvents may damage electrical cables.
  • Page 28 ➔ If your module was shipped in cold weather, leave it in its box and allow it to warm slowly to room temperature to avoid condensation. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 29: Physical Specifications

    Auto-ignition temperature ≥200 °C If a sample cooler is included the upper value for humidity can be reduced. Please check your lab conditions to stay beyond dew point values for non–condensing operation. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 30: Performance Specifications

    Site Requirements and Specifications Performance Specifications Performance Specifications Table 2 Performance Specifications 1260 Infinity II Preparative Autosampler (G7157A) Type Specification Comment Settable injection 0.1 – 900 µL, up to 3600 µL with seat extension range 1 µL: <5 % Injection precision Measured caffeine 5 µL: <2 %...
  • Page 31 Site Requirements and Specifications Performance Specifications Table 2 Performance Specifications 1260 Infinity II Preparative Autosampler (G7157A) Type Specification Comment Instrument Control LC & CE Drivers A.02.17 or above For details about supported software versions Instrument Control Framework (ICF) A.02.04 or...

  • Page 32: Specifications Of The Sample Cooler

    Up to 4600 m (15091 ft) Safety standards: Installation category II, For indoor use only. IEC, EN, CSA, UL Pollution degree 2 ISM Classification ISM Group 1 Class B According to CISPR 11 Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 33 4 °C to 5 °C below ambient Temperature settable from 4 – 40 °C in 1 ° increments Temperature accuracy (<25 °C, <50 % r.H.) 2 °C to 6 °C at a setpoint of 4 °C Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 34 Site Requirements and Specifications Specifications of the Sample Cooler Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 35: Using The Module

    Agilent 1260 Infinity II Preparative Autosampler User Manual Using the Module Magnets Turn on/off Status Indicators Exchange Drawers Install the External Tray Sample Trays Choice of Vials and Caps Transporting the Sampler with a Sample Cooler Installed Agilent Local Control Modules This chapter provides information on how to use the module.

  • Page 36: Magnets

    Using the Module Magnets Magnets This stack exemplarily shows the magnets' positions in the modules. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 37: Turn On/Off

    Using the Module Turn on/off Turn on/off Power switch: On Turn instrument On/Off with the control software. Power switch: Off Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 38 Using the Module Turn on/off Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 39: Status Indicators

    5. Resident mode (blinking) - for example during update of main firmware. 6. Bootloader mode (fast blinking). Try to re-boot the module or try a cold-start. Then try a firmware update. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 40: Exchange Drawers

    Install classic drawer 1-50 to the left, classic drawer 51-100 to the right side. Open the front door. Pull the drawer out. Lift the front of the drawer. Lift the drawer out. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 41 Using the Module Exchange Drawers Remove the drawer. Insert the back of the drawer. Align the drawer. Push in the drawer. Close the doors. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 42: Install The External Tray

    For best temperature performance, and if the external tray is not in use, it is best to cover the opening for the external tray with the original parts. Lift the foam with a flathead screwdriver. Remove the foam. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 43 Using the Module Install the External Tray Push out the plastic cover. Insert the external tray. Vent for the external tray is open. Install the disposal tube. Close the door. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 44: Sample Trays

    Drawers can be installed in any combination enabling both 2 mL- and 6 mL-vials to be used simultaneously. The only exception is the usage of the classical drawer option (100 x 2 mL). This option can't combine with the other drawers. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 45 No. 206) Figure 9 Numbering of drawer position (left-hand Drawer for 66 x 2 mL Vials) Figure 10 Numbering of drawer position (right-hand Drawer for 66 x 2 mL Vials) Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 46 Numbering of drawer position (left-hand Drawer for 18 x 6 mL Vials) Figure 12 Numbering of drawer position (right-hand Drawer for 18 x 6 mL Vials) Figure 13 Numbering of drawer position (Drawer for 50 x 2 mL Vials Classic) Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 47 Using the Module Sample Trays Figure 14 Numbering of tray position (External tray) Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 48: Choice Of Vials And Caps

    (silanized) 5182-0567 Crimp Top Vial, 1 mL, polypropylene, wide opening, 100/Pack 5183-4496 Crimp Top Vial, 1 mL, polypropylene, wide opening, 100/Pack (silanized) 9301-0978 crimp top vial, 0.3 mL, polypropylene, wide opening, 1000/pack Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 49 Screw Cap Vial, 2 mL, amber glass, write-on spot, 100/pk 5183-2069 Screw Top Vial, 2 mL, amber glass, write-on spot, 1000/Pack 5183-2072 Screw Top Vial, 2 mL, amber glass, write-on spot, 100/Pack (silanized) Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 50 Snap Cap, clear polypropylene, septum (clear PTFE/red rubber), 100/Pack 5182-3458 Snap Cap, blue polypropylene, septum (clear PTFE/red rubber), 100/Pack 5182-3457 Snap Cap, green polypropylene, septum (clear PTFE/red rubber), 100/Pack 5182-3459 Snap Cap, red polypropylene, septum (clear PTFE/red rubber), 100/Pack Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 51 Screw Cap, red polypropylene, septum (clear PTFE/red rubber), 100/Pack 5182-0720 Screw Cap, blue polypropylene, septum (clear PTFE/silicone), 100/Pack 5182-0721 Screw Cap, green polypropylene, septum (clear PTFE/silicone), 100/Pack 5182-0722 Screw Cap, red polypropylene, septum (clear PTFE/silicone), 100/Pack Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 52: Transporting The Sampler With A Sample Cooler Installed

    Ensure that you can cope with the weight of your load. Transporting the sampler with a sample cooler installed is only allowed for short N O T E distances. For longer distances, you must separate the units and send them independently. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 53 Advisor online help for more information. Also ensure there is no vial in the gripper arm. • Install the transport protection. • Remove all vials from the drawers. • The condensed water inside of the sample cooler is removed. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 54: Agilent Local Control Modules

    • fast and direct control in front of the instrument • a clear overview of the system status • control functionalities • access to method parameters and sequences • a logbook showing events from the modules • diagnose tests Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 55 • GLP – System logbook and module log-books record errors, unusual events and maintenance activities for GLP traceability. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 56 Using the Module Agilent Local Control Modules Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 57: Installation Of The Optional Sample Cooler

    Agilent 1260 Infinity II Preparative Autosampler User Manual Installation of the Optional Sample Cooler Injector Step Commands Damaged Packaging Install the Sample Cooler Using the Sample Cooler in an Infinity II Sampler Control Interface Control Temperature Mode Online Signal Monitor This chapter describes the installation of the optional sample cooler.

  • Page 58: Injector Step Commands

    Needle into Seat Lowers the needle arm into the seat. Command also returns the vial to the tray position. Valve Mainpass Switches the injection valve to the mainpass position. Reset Resets the injector. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 59: Damaged Packaging

    Damaged Packaging Damaged Packaging If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the instrument may have been damaged during shipment. "Defective on arrival" problems C AU T I O N If there are signs of damage, please do not attempt to install the module.

  • Page 60: Install The Sample Cooler

    A suitable container must be provided and emptied regularly in order to avoid overflow. For best cooling performance of the sampler all drawers must be installed in the module. N O T E Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 61 Ensure that the power switch on the front of the module Ensure that the power cable is removed from the is OFF (switch stands out). instrument. Open the 4 screws on the rear of the module. Remove the sample cooler mainframe cover. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 62 To avoid damages of the electronics of the module make sure the power cords are unplugged before disconnecting or reconnecting the sampler to the sample cooler cables. Connect power cable and signal/data cable. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 63 Fit in the sample cooler perfectly. Slide in the whole unit. Use a bubble level to check the leveling of the sampler. N O T E The sample cooler should be operated in a proper horizontal position. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 64 Wait at least 30 min before switching on the compressor of the sample cooler. ➔ This allows the refrigerant and system lubrication to reach equilibrium. Connect the power cable to the power connector at the rear of the module. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 65: Using The Sample Cooler In An Infinity Ii Sampler

    Drawer Configuration EMF Status ALS configuration is displayed when moving with the mouse cursor over the syringe. The information provides ALS related information like • Part number • ALS setup and other details. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 66: Control Interface

    Installation of the Optional Sample Cooler Using the Sample Cooler in an Infinity II Sampler Control Interface A right-click into the active area will open a menu to show the Control Interface. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 67: Control

    Select On to switch on the cooler. Specify the required temperature in the adjacent field. Note that the specified temperature must be at least 5 °C below ambient for proper temperature control. Select Off to switch off the cooler. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 68: Temperature Mode

    This mode may be used for degradation experiments (this solution is not officially recommended by Agilent) or as a workaround for reporting. Reporting of control parameters will become available with the CDS and ICF. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 69: Online Signal Monitor

    The online signal monitor for the chiller is also available and allows you to monitor the WPS1A temperature signals. The Online Plot Window should look like this: If the change button is pressed you can select the Chiller signal WPS1A in the online plot. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 70 This will disappear after the unit comes out of the heating cycle and goes back into normal operation. Shut down of the sample cooler at low temperatures will build up some condensate inside the autosampler. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 71 Adjusting the set points from a colder to a warmer set point will result in some N O T E condensation. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 72 Installation of the Optional Sample Cooler Using the Sample Cooler in an Infinity II Sampler Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 73: Preparing The Module

    Agilent 1260 Infinity II Preparative Autosampler User Manual Preparing the Module Leak and Waste Handling Preparing the Module Solvent Information Capillary Color Coding Guide Swage Fittings Flow Connections to the sampler Setting up the Sampler Control Settings Method Parameter Settings...

  • Page 74: Leak And Waste Handling

    N O T E has been shipped with the solvent cabinet, electronic copies are available in the Agilent Information Center or via the Internet. For details on correct installation, see separate installation documentation. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 75: Preparing The Module

    To clean the system when Bidistilled water Best solvent to re-dissolve buffer using buffers crystals After a solvent change Bidistilled water Best solvent to re-dissolve buffer crystals Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 76: Solvent Information

    HPLC analysis over several decades. These materials exhibit excellent robustness under typical HPLC conditions. For any special condition, please consult the material information section or contact Agilent. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 77 PEEK capillaries keeps the flow path free of steel and ensures pressure stability to at least 600 bar. If in doubt, consult the available literature about the chemical compatibility of PEEK. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 78 2.3. It can also corrode in following solvents: • Solutions of alkali halides, their respective acids (for example, lithium iodide, potassium chloride, and so on) and aqueous solutions of halogens. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 79 . Titanium is subject to corrosion in anhydrous chlorides like FeCl methanol, which can be avoided by adding a small amount of water (about 3 %). Slight corrosion is possible with ammonia > 10 %. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 80 (perfluoroalkoxy) and FEP (fluorinated ethylene propylene) are inert to almost all common acids, bases, and solvents. FFKM is perfluorinated rubber, which is also resistant to most chemicals. As an elastomer, it may swell in some organic solvents like halogenated hydrocarbons. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 81 Sapphire, Ruby and Al -based ceramics Sapphire, ruby and ceramics based on aluminum oxide Al are inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 82: Capillary Color Coding Guide

    Preparing the Module Capillary Color Coding Guide Capillary Color Coding Guide Figure 15 Syntax for capillary description Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 83: Swage Fittings

    N O T E Don't overtighten. Overtightening will shorten the lifetime of the fitting. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 84 If changing from a column or an injection valve to another, the fitting may leak or decrease the quality of the separation by contributing to band broadening. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 85: Flow Connections To The Sampler

    4 Route the drainage of the washport outlet to the waste container. 5 Prime the wash tubing. 6 Install the capillary from the pump outlet into the port 1 of the injection valve. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 86 7 Install the capillary from port 6 of the injection valve to the ICC or MCT The correct plumbing is shown in the figure below and is attached to the module. 8 Prime and purge the complete sampler. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 87: Setting Up The Sampler

    • Needle Wash: allows you to wash the needle without modifying your current method. The needle wash specified in this dialog box is carried out immediately. • Modify • Flow Path • External Tray • Temperature Mode Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 88 EMF Status shows Run / Ready / Error state and “Not Ready text” or “Error text” • Offline (gray) • Ok No Maintenance required (green) • EMF warning. Maintenance might be required (yellow) • EMF warning. Maintenance required (red) Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 89 Preparing the Module Setting up the Sampler Figure 17 ChemStation Method and Run Control Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 90: Control Settings

    For additional help and support, highlight the desired area and press the F1 key. A help N O T E screen will open with additional information and documentation about the topic. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 91: Method Parameter Settings

    Preparing the Module Setting up the Sampler Method Parameter Settings The method parameter settings are available via right click on the active area of the grafical user interface. Figure 19 Method parameter settings Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 92 Posttime to delay the start of the next analysis. A Posttime period can be used to allow the column to equilibrate after changes in solvent composition (for example after gradient elution). Limits: 0.01 through 99999 min or Off. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 93 (in mm) away from its standard position. The Offset function is useful when analyzing very small sample volumes, or when only a specific part of the sample is required, for example, the top layer. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 94 For additional help and support, highlight the desired area in the user interface and press N O T E the F1 key. A help screen will open with additional information and documentation about the topic. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 95: Injector Programm

    The following functions are available : • Draw • Eject • Mix • Inject • Move • Wait • Valve • Needle • Wash • Remote • Wait For • Repeat . End Repeat • Comment Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 96: Module Configuration View

    N O T E screen will open with additional information and documentation about the topic. The vialsampler configuration is done in the module dashboard context menu, not in the N O T E instrument configuration. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 97: Optimizing Performance

    Agilent 1260 Infinity II Preparative Autosampler User Manual Optimizing Performance Optimization for Lowest Carry-over Using the Automated Needle Wash (Wash Port) Using an Injector Program General Recommendation to Lowest Carry-Over Fast Injection Cycle and Low Delay Volume Overlapped Injection Mode...

  • Page 98: Optimization For Lowest Carry-Over

    It has a volume of ca. 0.5 mL and the peristaltic pump delivers ca. 5 mL/min, which means the wash port volume is completely refilled with fresh solvent in ca. 6 s. If the wash port is selected, the user can set the length Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 99 Line 1 moves the needle to vial P2-A1 or the flushport. Line 2 draws 5 μL from the current sample vial P1-A1. Line 3 injects the sample (valve switches to main pass). Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 100: Using An Injector Program

    Wait (equilibration time - see text above) Valve bypass Wait 0.2 min Valve mainpass Valve bypass Valve mainpass Overlapped injection together with additional injection valve switching is not possible. N O T E Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 101: General Recommendation To Lowest Carry-Over

    • Clean the needle seat with a tissue and remove all liquid. Again be careful not to risk an injury by the uncovered needle. • Reinstall the safety cover of the sampler. • In LabAdvisor, go to Maintenance > Change Needle and finish the procedure. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 102: Fast Injection Cycle And Low Delay Volume

    100 μl instead of 1 μl, increase the injection time by approximately 8 sec. In this case and if the viscosity of the sample allows it, the draw and eject speed of the injection system has to be increased. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 103: General Recommendations For Fast Injection Cycle Times

    • Increase of draw and eject speed for large injection volumes • Add at last run a blank, if overlapped injection is used To reduce the injection time, the detector balance has to be set to OFF. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 104: Precise Injection Volume

    A DRAW statement in an injector program also uses the draw speed setting which is configured for the autosampler. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 105 An EJECT statement in an injector program also uses the eject speed setting which is configured for the autosampler. A faster eject speed shortens the time required to run the injector program. When using viscous samples, a high eject speed should be avoided. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 106: Choice Of Rotor Seal

    It is suitable for application using mobile phases within a pH between 1 and 14. Strong oxidizing acids such as concentrated nitric and sulfuric acids are not compatible N O T E with PEEK. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 107: Troubleshooting And Diagnostics

    Agilent 1260 Infinity II Preparative Autosampler User Manual Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions Maintenance Functions User Interface Change Needle Change Metering Seal or Piston Park Arm Change Gripper Automatic Referencing Sampler Gripper Verification Test...

  • Page 108: Overview Of The Module's Indicators And Test Functions

    Tray alignment is required after repair of internal components. The procedure aligns the gripper arm correctly to ensure the positioning of the gripper arm is correct for all vials (see “Automatic Referencing” on page 117). Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 109 The step functions provide the possibility to execute each step of the sampling sequence individually. The step functions are used primarily for troubleshooting, and for verification of correct autosampler operation after repair (see “Step Commands” on page 119). Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 110: Maintenance Functions

    Change Gripper: The change gripper function moves the gripper to the front of the autosampler enabling easy access to the gripper release mechanism. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 111: Change Needle

    The needle arm moves in the desired position and guide the user to the next procedure step. Read carefully the desciption in the LabAdvisor user interface. N O T E Move Down The needle arm moves slowly down. Move Up The needle arm slowly moves up. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 112 5 Next positions the needle to the top level. Flap the safety wash port back in its origin position. 6 Reinstall the safety cover and complete the procedure. 7 Select Next to move the needle into the needle seat. 8 Select Back to complete the procedure. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 113: Change Metering Seal Or Piston

    3 Select Next to move the piston back to the home position. Read carefully the description in the LabAdvisor user interface. N O T E 4 Select Back to finish the procedure. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 114: Park Arm

    Control display. The commands for the control software are: Park Arm moves the gripper arm to the park position. Home moves the gripper arm out of the park position to the home position. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 115 Gripper function to remove the vial. 1 Select Park Arm. 2 When the arm is in the park position, install Transport-Protection (G7129-40050) . Then the autosampler is ready for shipment, and can be switched OFF. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 116: Change Gripper

    2 Exchange the gripper arm (see “Exchange the Gripper Arm” on page 177). 3 Select Next to move the gripper arm to the home position. 4 Select Back to finish the gripper procedure. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 117: Automatic Referencing

    Mount the calibration fixture. Position P1-C6 and P2-F6. Please check help for further explanation. N O T E Next Mount a 2 mL vial. Next Perform autoreferencing. Next Remove calibration fixture. Back Completes the procedure. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 118: Sampler Gripper Verification Test

    Automatic Referencing procedure should be done. Allows you to select a vial position. You can select from positions 1, 11, 39, 56, 66, 101, 111, 139, 156 and 166. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 119: Step Commands

    Needle into Seat Lowers the needle arm into the seat. Command also returns the vial to the tray position. Valve Mainpass Switches the injection valve to the mainpass position. Reset Resets the injector. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 120: Tests

    0.5 °Cover a 10 s period) the cooler is turned on and measurement is started. For the test to succeed, three temperature checkpoints must be reached within a specified time. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 121: Troubleshooting

    You can use the injector steps to do the injection sequence, while observing how the instrument responds. “Automatic Referencing” on page 117 summarizes the injector steps, and lists the associated error messages and probable causes of step failures. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 122: Troubleshooting Guide For The Sample Transport Assembly

    • motor overtemp (0 or 2 or 3) • movement failed (0 or 2 or 3) • missing vial Motor 0=X; 1=Z; 2=Theta; 3=Gripper. N O T E Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 123: Intermittent Lock-Ups With Or Without Vial In The Gripper Fingers

    (G7129-65800)) or the fusion board ( Fusion Board (G4200-65880)). 7 For both steps (step 5 and 6), you have to do an automatic referencing again to store the correct values in the instrument firmware again. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 124: Poor Alignment

    (G7129-65800)) or the fusion board ( Fusion Board (G4200-65880)). 5 For all three steps (step 2, 3, and 4) you have to do an automatic referencing again to store the correct values in the instrument firmware again. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 125: Agilent Lab Advisor Software

    The tests and diagnostic features that are provided by the Agilent Lab Advisor software may differ from the descriptions in this manual. For details, refer to the Agilent Lab Advisor software help files. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 126 Troubleshooting and Diagnostics Agilent Lab Advisor Software Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 127: Error Information

    Agilent 1260 Infinity II Preparative Autosampler User Manual Error Information What are Error Messages General Error Messages Timeout Shutdown Remote Timeout Lost CAN Partner Leak Sensor Leak Sensor Open Leak Sensor Short Compensation Sensor Open Compensation Sensor Short Fan Failed...
  • Page 128 Cooler temperature below or above target temperatur. Cooler Overpressure Failure Cooler Condensate Error This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 129: What Are Error Messages

    In all cases, error propagation is done via the CAN bus or via an APG/ERI remote cable (see documentation for the APG/ERI interface). Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 130: General Error Messages

    Check the vacuum degasser for an error The degasser failed to generate sufficient condition. Refer to the Service Manual for the vacuum for solvent degassing. degasser or the pump that has the degasser built-in. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 131: Remote Timeout

    Exchange the CAN cable. Defective CAN cable. Switch off the system. Restart the system, and Defective main board in another module. determine which module or modules are not recognized by the system. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 132: Leak Sensor

    Exchange the peristaltic pump. Leaking peristaltic pump The leak sensor in the sampler is hidden under the ICC Column Heater or Column Shelf N O T E respectively. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 133: Leak Sensor Open

    Switch board. Exchange the leak sensor. Defective leak sensor. Exchange the leak sensor. Leak sensor incorrectly routed, being pinched by a metal component. Exchange the power switch assembly. Power switch assembly defective Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 134: Leak Sensor Short

    Check the ribbon cable between the power Loose connection between the power switch board and the main board. switch board and the main board Exchange the power switch assembly. Defective power switch assembly Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 135: Compensation Sensor Short

    Probable cause Suggested actions Ensure the fan is connected correctly. Fan cable disconnected. Exchange fan. Defective fan. Replace the sample cooler. Defective sample cooler fan Exchange the main board. Defective main board. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 136: Sampler Error Messages

    If the valve fails to reach the mainpass position, or if the microswitch does not close, the error message is generated. Probable cause Suggested actions Exchange the injection valve. Defective injection valve. Exchange the main board. Defective main board. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 137: Needle Movement Failed

    Align gripper. Incorrect gripper alignment. Exchange the gripper assembly. Defective gripper assembly (defective gripper fingers or belt). Exchange the transport assembly. Defective transport assembly flex board. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 138: Initialization Failed

    Exchange and reset the needle station board. Defective needle station board. Exchange the transport assembly. Defective transport assembly flex board. Exchange the hydraulic unit motor. Defective hydraulic unit motor. Exchange the main board. Defective main board. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 139: Metering Home Failed

    Exchange the metering piston and seal. Broken piston. Exchange the metering-drive motor. Defective metering-drive motor. Exchange the main board. Defective main board. • Check belt tension. Loose belt. • Exchange the belt Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 140: Motor Temperature

    Switch OFF the autosampler at the power Motor belt tension too high. switch. Wait at least 10 min before switching on again. Exchange the transport assembly. Defective motor. Exchange the transport assembly. Defective transport assembly flex board. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 141: Initialization With Vial

    Probable cause Suggested actions Remove the vial using the Release Vial Vial still in gripper. function in the user interface. Reinitialize the autosampler. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 142: Missing Wash Vial

    Tray recognition defective (dirty sample tray are clean (located at the rear of the sample or defective transport assembly flex board). tray). If this does not solve the problem, replace the transport assembly. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 143: Valve Switch Not Found

    (mainpass or bypass), or if the sensor fails to recognize the valve position, the error message is generated. Probable cause Suggested actions Exchange the rotor seal/stator head. Defective rotor seal/stator head. Exchange the injection valve. Defective injection valve. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 144: Sample Cooler Error Messages

    • Check the power cable from the ALS to the Blown fuses, defective cable sample cooler. • Check if a fuse is blown (LED status on the specific mainboard). Replace the sample cooler. Defective PCB boards Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 145 Probable cause Suggested actions Switch off the instrument and wait for 15 min. Overpressure in the chiller Restart the instrument and check the signals in Lab Advisor. Replace the sample cooler. Defective compressor Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 146 • Switch the sample cooler off/on and check Defective condensate sensor if after 8 min the error shows up again. • Replace the condensate sensor. Replace the sample cooler. Defective leak sensor Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 147: Maintenance

    Agilent 1260 Infinity II Preparative Autosampler User Manual Maintenance Introduction to Maintenance Warnings and Cautions Overview of Maintenance Cleaning the Module Remove and Install Doors Exchange the Needle Assembly Exchange the Needle Seat Assembly Exchange the Sample Loop Assembly Exchange the Rotor Seal...

  • Page 148: Introduction To Maintenance

    Status indicator Needle assembly Drawer Column shelf Leak drain Power switch There are no serviceable parts inside. N O T E Do not open the module. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 149: Warnings And Cautions

    Sharp metal edges WA R N I N G Sharp-edged parts of the equipment may cause injuries. ➔ To prevent personal injury, be careful when getting in contact with sharp metal areas. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 150 Avoid back strain or injury by following all precautions for lifting heavy objects. ➔ Ensure that the load is as close to your body as possible. ➔ Ensure that you can cope with the weight of your load. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 151: Overview Of Maintenance

    Table 11 Overview of maintenance Procedure Typical interval (minimum) Notes Change needle/needle seat 30000 needle into seat movements Change peristaltic pump 3000 min on time cartridge Change rotor seal 30000 injections Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 152: Cleaning The Module

    WA R N I N G hazard and damage the module ➔ Do not use an excessively damp cloth during cleaning. ➔ Drain all solvent lines before opening any connections in the flow path. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 153: Remove And Install Doors

    Press the release buttons and pull the front door out. For the Installation of the front door, insert the hinges into their guides and move the door in until the release buttons click into their final position. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 154: Exchange The Needle Assembly

    Wear safety goggles and safety gloves when removing the needle assembly. It is recommended to always exchange the needle assembly and the needle seat at the N O T E same time to prevent premature leakage. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 155 Unscrew the safety cover screw. WA R N I N G Sharp needle Uncovered needles may cause injuries ➔ Do not touch the tip of the needle. Remove the safety cover. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 156 Turn or flap the wash port to the left. The needle will move slowly downwards and will stop approximately 2 mm above the seat. Disconnect the sample loop from the needle. Loosen the fixing screw. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 157 N O T E Do not overtighten the fitting! N O T E Incorrect positioning and installation of the needle/loop connection can result in damaging and breaking the sample loop. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 158 Install the safety cover (1) and fix the screw (2). Select Next to move the needle into the needle seat. Select Back to finish the procedure. Close the doors. Perform a pressure test. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 159: Exchange The Needle Seat Assembly

    In the Agilent Lab Advisor software select Service & Open the front door. Diagnostics in the system screen (Tools) Maintenance Positions > Change Needle/Loop, click Start and wait until the needle assembly is in maintenance position. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 160 Do not touch the tip of the needle. Turn or flap the wash port to the left. The needle will move slowly downwards and will stop approximately 2 mm above the seat. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 161 Disconnect the seat-capillary fitting from the injection valve (port 5). Use a small flat-head screwdriver to ease out the needle Insert the new needle-seat assembly. Press the seat seat. firmly into position. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 162 N O T E Do not overtighten the fitting! N O T E Incorrect positioning and installation of the needle/loop connection can result in damaging and breaking the sample loop. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 163 Install the safety cover (1) and fix the screw (2). Select Next to move the needle into the needle seat. Close the doors. Select Back to finish the procedure. Perform a pressure test. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 164: Exchange The Sample Loop Assembly

    Change loop function. In the Agilent Lab Advisor software select Service & Diagnostics in the system screen Maintenance Positions > Change loop, click Start and wait until the needle assembly is in maintenance position. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 165 Do not touch the tip of the needle. Remove the safety cover. Check if the needle is in maintenance position. Disconnect the loop capillary from the metering device and from the needle. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 166 Install the safety cover (1) and fix the screw (2). Click Next until the needle assembly is moved to the Close the doors. needle seat. Select Back to finish the procedure. Perform a pressure test. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 167: Exchange The Rotor Seal

    The stator face is held in place by the stator head. When you remove the stator head, the stator face can fall out of the valve. ➔ Carefully handle the valve to prevent damage to the stator face. ➔ Carefully handle the stator face during sonication. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 168 Remove all capillaries from the injection valve with a 1/4 inch wrench. Loosen each fixing bolt two turns at a time. Remove the Remove the stator head and stator ring. bolts from the head. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 169 N O T E Make sure that the rotor sealing surface with its engraved flow passages is facing out. The pattern is asymmetrical to prevent improper placement. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 170 Do not over-tighten the screws. The screws hold the assembly together and do not affect the sealing force. The sealing force is automatically set as the screws close the stator head against the valve body. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 171 Reconnect the capillaries and tubes to the valve ports. Slide the waste tube into the waste holder in the leak tray. The correct plumbing is shown below and on the safety cover of the module. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 172 Maintenance Exchange the Rotor Seal Close the doors. Perform a pressure test. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 173: Exchange The Metering Seal And Piston

    In the Tools section of the Agilent Lab Advisor software Open the front door. select Service & Diagnostics > Maintenance Positions > Change Metering Device, click Start and wait until the metering device is in maintenance position. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 174 Pull the metering head assembly away from the sampler. Remove the two fixing bolts from the base of the metering head assembly. Disassemble the metering head assembly. Use a seal insert tool to carefully remove the seal. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 175 Ensure the large hole in the metering head is facing stator head is secure. The housing must flush with the downwards. Tighten the bolts alternately two turns at a metering head. time until the metering device is secure. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 176 Service & Diagnostics > Maintenance Positions > Change Metering Device: click Next and wait until the metering device is in Home position. Select Back to finish the procedure. Close the doors. Perform a pressure test. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 177: Exchange The Gripper Arm

    Identify the slit below the gripper motor and the gripper Rotate the arm approximately 2.5 cm (1 inch) to the left arm release button. and insert the straightened paper clip into the slit. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 178 Close the doors. In the Agilent Lab Advisor software select Change gripper > Next, click Next and wait until the gripper is in the default position. Select Back to finish the procedure. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 179: Replace The Peristaltic Pump Cartridge

    The peristaltic pump cartridge is a replaceable unit. The tubing inside the pump is not N O T E replaceable. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 180 Push the new cartridge onto the motor shaft until the Connect the wash port tubing to the upper tubing of the clips click into place. new cartridge (use sand paper to get a good grip on the tubing). Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 181 Connect the inlet filter of the solvent bottle. Use the Close the doors. syringe to draw enough solvent for completely filling of the peristaltic pump tubing before continuing to prime the peristaltic pump. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 182: Exchange The Wash Port Assembly

    Diagnostics in the system screen (Tools) Maintenance Positions > Change Needle/Loop, click Start and wait until the needle assembly is in maintenance position. Check if the needle is lifted up. Unscrew the safety cover screw. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 183 In Lab Advisor use Next to move the needle in the down Disconnect the sample loop from the needle. position. The needle will move slowly downwards and will stop approximately 2 mm above the seat. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 184 Disconnect the wash tubing from the peristaltic pump. Release the spring loaded pin. Lift the flapper out of the bracket. Be careful not to Reconnect the wash tubing fitting to the new wash port squeeze the wash tubing. assembly. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 185 (2). N O T E Do not overtighten the fitting! N O T E Incorrect positioning and installation of the needle/loop connection can result in damaging and breaking the sample loop. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 186 Select Next to move the needle into the needle seat. Select Back to finish the procedure. Close the doors. Purge the wash port. Check if solvent will be delivered from the peristaltic pump. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 187: Replace The Module Firmware

    Agilent web. http://www.agilent.com/en-us/firmwareDownload?whid=69761 2 For loading the firmware into the module follow the instructions in the documentation. Module Specific Information There is no specific information for this module. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 188 Maintenance Replace the Module Firmware Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 189: Parts And Materials For Maintenance

    Agilent 1260 Infinity II Preparative Autosampler User Manual Parts and Materials for Maintenance Main Assemblies Standard Parts Drawer Assembly External Tray Analytical-Head Assembly (900 µL) Standard Sampler Accessory Kit Multi-Draw Kit Additional Part List This chapter provides information on parts for maintenance.

  • Page 190: Main Assemblies

    Drawer for 50 x 2 mL Vials Classic Right 5067-4277 2pos/6port Injection Valve, prep, 600 bar G1313-60010 Gripper assembly G7129-60083 Analytical Head Assembly 900 µL 5065-4445 Peristaltic pump with Pharmed tubing Figure 21 Main assemblies Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 191: Standard Parts

    Finger caps (x3) G7157-60515 Loop for Prep-Autosampler G7157-87101 Seat Assy High Flow G7157-87201 Needle High Flow (slotted), indicated by a white ring 5067-4277 2pos/6port Injection Valve, prep, 600 bar Reorder gives pack of 15 Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 192: Drawer Assembly

    Drawer for 50 x 2 mL Vials Classic Right G7129-68210 Drawer-Kit 100 x 2 mL Vials Classic Figure 22 Drawer for 66 x 2.0 mL Vials Figure 23 Drawer for 18 x 6 mL Vials Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 193 It is required that all drawers are put into correct positions, that is, classical drawer 1-50 N O T E must be on the left side and classical drawer 51-100 on the right side. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 194: External Tray

    Parts and Materials for Maintenance External Tray External Tray Description G7129-60000 External Tray for 5 x 2 mL Vials G1313-27302 Disposal tube Figure 25 External tray Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 195: Analytical-Head Assembly (900 Μl)

    G7129-27790 Analytical-Head 900 µL 0905-1294 Metering seal, 900 µL 5001-3764 Support seal assembly, 900 µL G7129-60006 Adapter-Assembly Analytical-Head 0515-0850 Screws G4267-60462 Piston Assembly, 900 µL Figure 26 Analytical head assembly (900 µL) Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 196: Standard Sampler Accessory Kit

    CAN cable, Agilent module to module, 1 m G1313-44101 Finger Cap 5500-1155 Tube Connector, 90 degree, ID 6.4 5500-1254 Tube connector 180 ° 5500-1251 Capillary ST 0.12 mmX 400 mm SL/SL 5043-1013 Tubing Clip Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 197: Multi-Draw Kit

    The internal volume of the seat capillary is 400 µL or 1400 µL. With the sample loop the N O T E maximum draw volume of 500 µL or 1500 µL can be reached. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 198: Additional Part List

    Parts and Materials for Maintenance Additional Part List Additional Part List Description G7129-68001 Insulation Kit G7129-68000 Air guide kit G7129-60014 Calibration Tool 5023-2540 Adapter ICC Diffusor G7116-67110 MCT temperature verification kit G7129-40050 Transport-Protection G7129-20025 Gripper adapter Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 199: Identifying Cables

    Agilent 1260 Infinity II Preparative Autosampler User Manual Identifying Cables Cable Overview Analog Cables Remote Cables CAN/LAN Cables RS-232 Cables This chapter provides information on cables used with the Agilent InfinityLab LC Series modules. Agilent Technologies...

  • Page 200: Cable Overview

    Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent 35900A A/D converter...
  • Page 201 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m USB cables Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 202: Analog Cables

    The other end depends on the instrument to which connection is being made. Agilent Module to 35900 A/D converters p/n 35900-60750 35900 Pin Agilent Signal Name module Not connected Shield Analog - Center Analog + Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 203 Pin Agilent Signal Name module Shield Shield Analog - Center Center Analog + Agilent Module to General Purpose p/n 01046-60105 Pin Agilent Signal Name module Not connected Black Analog - Analog + Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 204: Remote Cables

    High grey NOT USED pink SHUT DOWN blue START PREPARE black 1wire DATA violet DGND grey-pink +5V ERI out red-blue PGND white-green PGND brown-green +24V ERI out white-yellow +24V ERI out yellow-brown Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 205 • 5188-8045 ERI to APG (Connector D_Subminiature 15 pin (ERI), Connector D_Subminiature 9 pin (APG)) p/n 5188-8045 Pin (ERI) Signal Pin (APG) Active (TTL) Start Request Stop Ready High Power on High Future Shut Down Start Prepare Ground Cable Shielding Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 206 Ground Cable Shielding One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 207 Wire Color Pin Agilent Signal Name Active module (TTL) White Digital ground Brown Prepare run Gray Start Blue Shut down Pink connected Yellow Power on High Ready High Green Stop Black Start request Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 208: Can/Lan Cables

    CAN cable, Agilent module to module, 1 m LAN Cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection) Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 209: Rs-232 Cables

    It's also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 210: Usb

    To connect a USB Flash Drive use a USB OTG cable with Mini-B plug and A socket. Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 211: Hardware Information

    Agilent 1260 Infinity II Preparative Autosampler User Manual Hardware Information Firmware Description Electrical Connections Rear View of the Module Serial Number Information Interfaces Overview Interfaces ERI (Enhanced Remote Interface) USB (Universal Serial Bus) Setting the 6-bit Configuration Switch Special Settings...

  • Page 212: Firmware Description

    • run synchronization through APG/ERI remote, • error handling, • diagnostic functions, • or module specific functions like • internal events such as lamp control, filter movements, • raw data collection and conversion to absorbance. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 213 Update of main system can be done in the resident system only. Update of the resident N O T E system can be done in the main system only. Main and resident firmware must be from the same set. Figure 27 Firmware Update Mechanism Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 214 All these specific informations are described in the documentation provided with the firmware update tools. The firmware update tools, firmware and documentation are available from the Agilent web. • http://www.agilent.com/en-us/firmwareDownload?whid=69761 Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 215: Electrical Connections

    There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply. Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations.

  • Page 216: Rear View Of The Module

    • CN = China Alphabetic character A-Z (used by manufacturing) Alpha-numeric code 0-9, A-Z, where each combination unambiguously denotes a module (there can be more than one code for the same module) 00000 Serial number Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 217: Interfaces

    Agilent InfinityLab LC Series Interfaces Module RS-232 Analog APG (A) Special (on-board) / ERI (E) Pumps G7104A/C G7110B G7111A/B, G5654A G7112B G7120A G7161A/B Samplers G7129A/B/C G7167B/C, G5667A G7157A Detectors G7114A/B G7115A G7117A/B/C G7121A/B G7162A/B G7165A Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 218 • RS-232C as interface to a computer • USB (Universal Series Bus) as interface to a computer • REMOTE connector as interface to other Agilent products • Analog output connector(s) for signal output Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 219: Overview Interfaces

    Remote (ERI) The ERI (Enhanced Remote Interface) connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 220 All common TTL circuits operate with a 5 V power supply. A TTL signal is defined as "low" N O T E or L when between 0 V and 0.8 V and "high" or H when between 2.0 V and 5.0 V (with respect to the ground terminal). Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 221: Eri (Enhanced Remote Interface)

    1090/1040/1050/1100 HPLC systems and Agilent 1100/1200/1200 Infinity HPLC modules. All new InfinityLab LC Series products using the FUSION core electronics use ERI. This interface is already used in the Agilent Universal Interface Box 2 (UIB2) Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 222 This way the interface can support various additional devices like sensors, triggers (in and out) and small controllers, etc. Figure 29 Location of the ERI interface (example shows a G7114A/B VWD) Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 223 This serial line can be used to read out an EPROM or write into an EPROM of a connected ERI-device. The firmware can detect the connected type of device automatically and update information in the device (if required). Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 224: Usb (Universal Serial Bus)

    • Short circuit will be detected through hardware. USB (Universal Serial Bus) USB (Universal Serial Bus) - replaces RS232, supports: • a PC with control software (for example Agilent Lab Advisor) • USB Flash Disk Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 225: Setting The 6-Bit Configuration Switch

    • For specific LAN modes switches 4-5 must be set as required. • For boot resident/cold start modes switches 1+2 or 6 must be UP. Figure 30 Location of Configuration switch (example shows a G7114A/B VWD) Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 226 When selecting mode COM, settings are stored to non-volatile memory. When selecting mode TEST, COM settings are taken from non-volatile memory. not assigned - Always keep these switches on position ‘0’ (off) Default IP Address is 192.168.254.11 Host Name will be the MAC address. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 227: Special Settings

    ➔ Save your methods and data before executing a forced cold start. If you use the following switch settings and power the instrument up again, it will start as described above. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 228 Boot Main System / Keep Data Boot Resident System / Keep Data Boot Main System / Revert to Default Data Boot Resident System / Revert to Default Data Note: The setting '0' (down) is essential. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 229: Instrument Layout

    • the plastic layers help cushion the electronic and mechanical parts from physical shock, and • the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 230: Early Maintenance Feedback (Emf)

    EMF limits, and then reset the EMF counters to zero. The next time the EMF counters exceed the new EMF limits, the EMF flag will be displayed, providing a reminder that maintenance needs to be scheduled. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 231 Agilent 1260 Infinity II Preparative Autosampler User Manual LAN Configuration What You Have to Do First TCP/IP parameter configuration Configuration Switches Initialization Mode Selection Dynamic Host Configuration Protocol (DHCP) General Information (DHCP) Setup (DHCP) Manual Configuration With Telnet PC and Agilent ChemStation Setup...

  • Page 232: Lan Configuration What You Have To Do First

    232) to • the PC network card using a crossover network cable (point-to-point) or • a hub or switch using a standard LAN cable. Figure 32 Location of LAN interfaces and MAC label Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 233: Tcp/Ip Parameter Configuration

    TCP/IP parameters after power-on. The parameters may be derived non-volatile memory or initialized with known default values. The initialization mode is selected by the configuration switch, see Table 17 page 235. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 234: Configuration Switches

    Location of Configuration switch (example shows a G7114A/B VWD) The module is shipped with all switches set to OFF, as shown above. To perform any LAN configuration, SW1 and SW2 must be set to OFF. N O T E Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 235: Initialization Mode Selection

    The TCP/IP connection will be established using these parameters. The parameters were configured previously by one of the described methods. Figure 34 Using Stored (Principle) Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 236 In the Using Default mode, the parameters stored in the memory of the module are not N O T E cleared automatically. If not changed by the user, they are still available, when switching back to the mode Using Stored. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 237: Dynamic Host Configuration Protocol (Dhcp)

    2 It may be necessary to fully qualify the hostname with the DNS suffix, e.g. 0030d3177321.country.company.com. 3 The DHCP server may reject the hostname proposed by the card and assign a name following local naming conventions. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 238: Setup (Dhcp)

    Card or the main board of above mentioned modules. Table 19 G1369C LAN Interface Card (configuration switch on the card) SW 4 SW 5 SW 6 SW 7 SW 8 Initialization Mode DHCP Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 239 Lab Advisor, Firmware Update Tool) and use MAC address as host name, e.g. 0030d3177321. The LC system should become visible in the control software (see Note in section “General Information (DHCP)” on page 237). Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 240: Manual Configuration

    Therefore, manual configuration can be done at any time. A power cycle is mandatory to make the stored parameters become the active parameters, given that the initialization mode selection switches are allowing Figure 38 Manual Configuration (Principle) Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 241: With Telnet

    When the connection was established successfully, the module responds with the following: Figure 40 A connection to the module is made 3 Type ? and press enter to see the available commands. Figure 41 Telnet Commands Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 242 Initialization mode is Using Stored active TCP/IP settings TCP/IP status - here ready connected to PC with controller software (e.g. Agilent Figure 42 Telnet - Current settings in "Using Stored" mode ChemStation), here not connected Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 243 If the Initialization Mode Switch is changed now to “Using Stored” mode, the instrument N O T E will take the stored settings when the module is re-booted. In the example above it would be 192.168.254.12. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 244: Pc And Agilent Chemstation Setup

    This procedure describes the change of the TCP/IP settings on your PC to match the module’s default parameters in a local configuration (see Table 18 on page 236). 1 Open the Local Area Connection Properties and select Internet Protocol (TCP/IP). Then click on Properties. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 245 LAN Configuration PC and Agilent ChemStation Setup 2 You may enter here the fixed IP address of the module or use the Alternative Configuration. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 246 LAN Configuration PC and Agilent ChemStation Setup 3 We will use the direct LAN access via Cross-over LAN cable with the module’s IP address. 4 Click on OK to save the configuration. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 247: Agilent Chemstation Setup

    N O T E Control Software. 1 Open the ChemStation Configuration Editor. 2 Select from the menu Configure - Instruments. 3 Select Modular 3D LC System. 4 Give the Instrument a name. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 248 LAN Configuration PC and Agilent ChemStation Setup 5 Click on OK. 6 Select LC System Access — Access Point and click on Add. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 249 9 Save the current configuration via File – Save. 10 Exit the Configuration Editor. 11 Start the Agilent ChemStation. During first startup or when the system configuration has changed, a notification shows up. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 250 14 Click on OK. The selected module is shown now in the right window (with serial number). In addition all other modules connected via CAN to the detector are shown as well. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 251 Under Connection Settings you may change the IP/Hostname of the module (may require a re-start of the ChemStation). After successful load of the ChemStation, you should see the module(s) as active item in the graphical user interface (GUI). Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 252 LAN Configuration PC and Agilent ChemStation Setup Figure 45 Screen After Successful Load of ChemStation Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 253: Appendix

    Agilent 1260 Infinity II Preparative Autosampler User Manual Appendix General Safety Information General Safety Information Safety Standards General Before Applying Power Ground the Instrument Ground Solvent Lines Do Not Operate in an Explosive Atmosphere Do Not Remove the Instrument Cover...

  • Page 254: General Safety Information

    Do not use this product in any manner not specified by the manufacturer. The protective features of this product may be impaired if it is used in a manner not specified in the operation instructions. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 255: Before Applying Power

    Verify that the voltage range and frequency of your power distribution matches to the power specification of the individual instrument. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. ➔...

  • Page 256: Ground Solvent Lines

    Do not install substitute parts or perform any unauthorized modification to the product. Return the product to an Agilent Sales and Service Office for service and repair to ensure that safety features are maintained. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 257: In Case Of Damage

    Personal injury (for example electrical shock, intoxication) ➔ Instruments that appear damaged or defective should be made inoperative and secured against unintended operation until they can be repaired by qualified service personnel. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 258: Solvents

    For details, see the usage guideline for the solvent cabinet. A printed copy of the guideline N O T E has been shipped with the solvent cabinet, electronic copies are available in the Agilent Information Center or via the Internet. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 259: Symbols

    A pacemaker could switch into test mode and cause illness. A heart defibrillator may stop working. If you wear these devices keep at least 55 mm distance to magnets. Warn others who wear these devices from getting too close to magnets. Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 260 C AU T I O N alerts you to situations that could cause loss of data, or damage of equipment. ➔ Do not proceed beyond a caution until you have fully understood and met the indicated conditions. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 261: Waste Electrical And Electronic Equipment Directive

    Monitoring and Control Instrumentation product. Do not dispose of in domestic household waste N O T E To return unwanted products, contact your local Agilent office, or see http://www.agilent.com for more information. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 262: Refrigerant

    The refrigerant HFC-134a is used only in the Agilent Infinity II Sample Cooler. Table 23 Physical properties of refrigerant HFC-134a Molecular weight Critical temperature 101.1 °C Critical pressure 40.6 bar Boiling point -26.5 °C Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 263 All media must be disposed of in accordance with national and local regulations. ➔ Please contact your local Agilent Service Center in regard to safe environmental disposal of the appliance or check www.agilent.com for more info. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 264: Radio Interference

    Appendix Radio Interference Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with equipment unscreened...

  • Page 265: Sound Emission

    This product has a sound pressure emission (at the operator position) < 70 dB. • Sound Pressure Lp < 70 dB (A) • At Operator Position • Normal Operation • According to ISO 7779:1988/EN 27779/1991 (Type Test) Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 266: Solvent Information

    (e.g. EDTA), • mixtures of carbon tetrachloride with 2-propanol or THF. • Avoid the use of dimethyl formamide (DMF). Polyvinylidene fluoride (PVDF), which is used in leak sensors, is not resistant to DMF. Agilent 1260 Infinity II Preparative Autosampler User Manual...

  • Page 267: Agilent Technologies On Internet

    Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 268 104, cable exchange DRAW 58, 104, capillaries drawers drawer caps drawers crimp exchange screw fan failed snap features change metering seal 113, G7157A eject speed 104, change needle function Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 269 Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 270 X-axis replacing firmware reset RS-232C TCP/IP parameter configuration cable Z-axis telnet configuration theta-axis safety class I timeout safety transport assembly general information transport standards troubleshooting Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 271 Index Agilent 1260 Infinity II Preparative Autosampler User Manual...
  • Page 272 In This Book This manual contains technical reference information about the Agilent 1260 Infinity II Preparative Autosampler (G7157A). The manual describes the following: • Introduction, • site requirements and specifications, • using the module, • optimizing performance, • troubleshooting and diagnostics, •...

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