Agilent Technologies 1260Infinity II Bio-inert FractionCollector User Manual page 44

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Using the Module
Configuration and Operation of the Fraction Collector
Upper Threshold
At high absorbance values the light intensity on the detector is extremely low
and consequently detector noise will be superimposed on the detector signal.
In this case the detector noise might trigger fraction collection. To avoid false
fraction collection triggering, we recommend setting an Upper Threshold well
below the limit where this false triggering effect might occur. As soon as the
detector signal exceeds the Upper Threshold, settings for Up Slope or Down Slope
will be ignored until the signal drops again below the Upper Threshold.
When using more than one peak detector fraction collection can be triggered
either when all selected peak detectors detect a peak or when at least one selected
peak detector detects a peak basing on the settings in the peak detectors table
above.
If an MSD is used for mass-based fraction collection, Use MSD for mass-based
Fraction Collection must be checked.
Timetable
The Timetable can be used to program changes in the Fraction Trigger Mode
during the analysis by entering a Time and specifying the trigger settings.
Trigger Mode Off, Peak Based and Time Based can be selected. If the Off is
selected, no fractions are collected. The last entry in the timetable has to be
the command Off.
Whenever the Peak Based mode is specified fractions will be collected based on
the peak detection parameters given in the Peak Detector table. Additionally a
Maximum Peak Duration in minutes has to be specified. This parameter is
mandatory if you use Peak Controlled fraction collection, but is disabled for
Time Based fraction collection.
When the Time Based mode is chosen two different options are available:
• The # of Fractions can be edited to collect a fixed number of equal fractions
• Timeslices [min] can be edited to collect fractions with a defined collection
For editing the Timetable the functions Insert, Append, Cut, Copy and Paste are
offered.
To access the additional sections in the Setup Fraction Collector dialog box click
More.
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in a give time interval. This time interval is defined by the time value in the
current and following timetable line.
time. With this option the collection time of the last fraction can be shorter.
This depends on the overall runtime.
1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual