Optimizing Fraction Collection - Agilent Technologies 1260Infinity II Bio-inert FractionCollector User Manual

Using High Test Tubes in the Analytical Fraction Collector
In the standard configuration of the analytical fraction collector, the
maximum test tube height is limited to 48 mm. This limitation can be extended
to 75 mm if
• the standard 50 mm needle assembly ( Analytical needle assembly
• the wellplate adapter is removed.

Optimizing Fraction Collection

Time-based fraction collection
• Time slices must have a length of at least 0.05 min.
• Set # of Fractions such that length of resulting fractions is at least 0.05 min.
Peak-based fraction collection
• Set threshold and slope values such that length of fractions is at least
• Unresolved peaks can be separated using appropriate threshold and slope
• If the baseline of the chromatogram is below or above 0 mAU, this offset is
1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual
(G1367-87200)) is replaced by the 20 mm needle assembly ( Analytical
needle assembly (G1364-87202)),
0.05 min.
values. If two unresolved peaks are to be collected as one fraction, collect
based on threshold only.
not accounted for when triggering peaks using a threshold value. The
threshold value is always added to 0 mAU.
Configuration and Operation of the Fraction Collector
3
Using the Module
51