Agilent Technologies 1260Infinity II Bio-inert FractionCollector User Manual page 43

3
Using the Module
Configuration and Operation of the Fraction Collector
Peak Detectors
In the Peak Detectors section a list of all peak detectors that are connected to
the system is displayed. Agilent InfinityLab LC Series diode array detectors,
multiwavelength detectors, variable wavelength detectors and fluorescence
detectors are recognized automatically. Other detectors, e.g. Agilent 6000
mass-selective detectors or HP1050 detectors, are connected through the
Universal Interface Box (UIB).
The peak detector table contains seven columns:
Working Mode
For each peak detector Threshold only, Threshold/Slope or Slope only are possible.
In the Threshold only mode the settings for Up Slope, Down Slope and Upper
Threshold in the subsequent columns are ignored. Fraction collection is
triggered whenever the detector signal exceeds the specified threshold value.
When the signal drops below the threshold value fraction collection is
stopped.
In the Slope only mode fraction collection is triggered on the slope of the
detector signal. Adequate values for Up Slope and Down Slope can be specified
in the corresponding fields.
In the Threshold/Slope mode fraction collection is triggered on the
corresponding values for threshold and slope. The fraction collection is
started if the detector signal exceeds both the threshold and the Up Slope
value. The fraction collection is stopped if the detector signal drops either
below the threshold or the Down Slope value.
To specify the trigger values Up Slope, Down Slope, Threshold and Upper Threshold
we recommend to use the Fraction Preview tool as described in
"Fraction
Preview" on page 46.
1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual 43