Page 1 Agilent InfinityLab LC Series Vialsamplers User Manual Agilent Technologies...
Page 2 (June 1987) or any equivalent agency regu- lation or contract clause. Use, duplication or disclosure of Software is subject to Agi- lent Technologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 3 This chapter explains the operational parameters of the module. 6 Optimizing Performance This chapter provides information on how to optimize the module. 7 Troubleshooting and Diagnostics Overview about the troubleshooting and diagnostic features. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 4 This chapter describes the detector in more detail on hardware and electronics. 13 LAN Configuration This chapter provides information on connecting the module to the Agilent ChemStation PC. 14 Appendix This chapter provides addition information on safety, legal and web. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 5: Table Of Contents
Install the External Tray Install a Column (ICC) Remove a Column (ICC) Sample Trays Choice of Vials and Caps Transporting the Sampler with a Sample Cooler or Sample Thermostat Installed Agilent Local Control Modules Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 6 Troubleshooting Guide for the Sample Transport Assembly Agilent Lab Advisor Software 8 Error Information What are Error Messages General Error Messages Sampler Error Messages Sample Cooler Error Messages Integrated Column Compartment (ICC) Heater Error Messages Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 7 2ps 6pt Injection Valve 800 bar 2ps 6pt Injection Valve 600 bar Integrated Column Compartment Cabinet Kit Sample Thermostat Standard Vialsampler Accessory Kit Multi-Draw Kit Additional Part List Column Identifier Kit 2C (G4751A) Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 8 Dynamic Host Configuration Protocol (DHCP) Manual Configuration PC and Agilent ChemStation Setup 14 Appendix General Safety Information Waste Electrical and Electronic Equipment (WEE) Directive Refrigerant Radio Interference Sound Emission Solvent Information Agilent Technologies on Internet Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 9: Introduction
Agilent InfinityLab LC Series Vialsamplers User Manual Introduction Product Description (G7129A) Features (G7129A) Product Description (G7129B) Features (G7129B) Product Description (G7129C) Features (G7129C) Overview of the Module Operating Principle Sampling Sequence Needle Parkstation Hydraulic Box Transport Assembly Leak and Waste Handling...
Page 10: Product Description (G7129A)
80 °C as well as a sample cooler/sample thermostat for stable temperatures down to 4 °C or from 4 °C up to 40 °C, all within one module. Figure 1 Overview of the Vialsampler Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 11: Features (G7129A)
"bypass" mode • Overlapped injections for increased productivity • Injection program available for customizing advanced injections as well as for sample preparation steps upfront injection Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 12: Product Description (G7129B)
LC columns with temperature control up to 80 °C as well as a sample cooler/sample thermostat for stable temperatures down to 4 °C or from 4 °C up to 40 °C, all within one module. Figure 2 Overview of the Vialsampler Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 13: Features (G7129B)
"bypass" mode • Overlapped injections for increased productivity • Injection program available for customizing advanced injections as well as for sample preparation steps upfront injection Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 14: Product Description (G7129C)
80 °C as well as a sample cooler/sample thermostat for stable temperatures down to 4 °C or from 4 °C up to 40 °C, all within one module. Figure 3 Overview of the Vialsampler Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 15: Features (G7129C)
"bypass" mode • Overlapped injections for increased productivity • Injection program available for customizing advanced injections as well as for sample preparation steps upfront injection Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 16: Overview Of The Module
Overview of the Module The Agilent InfinityLab LC Series Vialsamplers are designed for use with other modules of the Agilent InfinityLab LC Series, 1200 Series, and 1100 Series LC, or with other LC systems if adequate remote control inputs and outputs are available.
Page 17 "cooled Vialsampler", the combination of the Vialsampler with the sample thermostat is called a "thermostated Vialsampler". For more details, refer to the 1290 Infinity Sample Cooler or Sample Thermostat documentation. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 18: Operating Principle
10 The needle moves back. only if automated needle wash is selected. If this feature is disabled, the gripper arm positions the sample vial directly below the needle (Step 4) and lowers the needle into the vial. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 19 This ensures that all parts in contact with sample are flushed during the run, thus minimizing carry-over. Figure 4 Mainpass (main path) Position - standard position during runs and when the sampler is idle Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 20 20). Solvent from the pump enters the valve unit at port 1, and flows directly to the column through port 6. Figure 5 Valve in bypass - needle in vial, metering device aspirates sample volume Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 21 In the next step, the needle is washed (see Figure 6 page 21). Figure 6 Outer face of needle getting washed in wash port Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 22 (main path) position, flushing the sample onto the column (see Figure 7 on page 22). Figure 7 Valve switches to mainpass (main path) - sample is transferred towards the LC column Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 23: Needle Parkstation
The upper and lower needle positions are detected by reflection sensors on the needle station board, while the needle-in-vial position is determined by counting the motor steps from the upper needle-sensor position. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 24: Hydraulic Box
The backward movement of the piston (driven by the spring) draws sample from the vial. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 25 Two microswitches monitor switching of the valve (bypass and mainpass (main path) end positions). No valve adjustments are required after replacing internal components. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 26: Transport Assembly
The opening and closing of the gripper fingers are driven by a stepper motor linked by a toothed belt to the planetary gearing inside the gripper assembly. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 27 These positions are used by the processor to calculate the actual motor position. An additional six reflection sensors for tray recognition are mounted on the flex board at the front of the assembly. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 28: Leak And Waste Handling
Introduction Leak and Waste Handling Leak and Waste Handling The Agilent InfinityLab LC Series has been designed for safe leak and waste handling. It is important that all security concepts are understood and instructions are carefully followed. The solvent cabinet is designed to store a maximum volume of 8 L solvent. The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 2 L.
Page 29 Introduction Leak and Waste Handling Figure 11 Infinity II Leak Waste Concept (Flex Bench installation) Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 30 Introduction Leak and Waste Handling Figure 12 Infinity II Single Stack Leak Waste Concept (bench installation) Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 31 Figure 13 Infinity II Two Stack Leak Waste Concept (bench installation) The waste tube connected to the leak pan outlet on each of the bottom instruments guides the solvent to a suitable waste container. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 32: Leak Sensor
The leak sensor in the sampler is hidden under the ICC Column Heater or Column Shelf N O T E respectively. Waste Guidance The waste drainage must go straight into the waste containers. The waste flow must not N O T E be restricted at bends or joints. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 33: Waste Concept
1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with 4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don't immerse in the liquid. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 34 Introduction Leak and Waste Handling Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 35: Site Requirements And Specifications
Agilent InfinityLab LC Series Vialsamplers User Manual Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications Performance Specifications (G7129A) Performance Specifications (G7129B) Performance Specifications (G7129C) Specifications of the Sample Cooler Specifications of the Sample Thermostat Specifications of the Integrated Column Compartment This chapter provides information on environmental requirements, physical and performance specifications.
Page 36: Site Requirements
➔ Make sure the power connector of the instrument can be easily reached and unplugged. ➔ Provide sufficient space behind the power socket of the instrument to unplug the cable. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 37 Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. Absence of ground connection WA R N I N G The absence of ground connection can lead to electric shock or short circuit.
Page 38 Ensure adequate ventilation: typical air exchange of 25 m /h per m of laboratory floor area. ➔ Keep all ventilation openings in the enclosure clear of obstructions. Do not block the openings on the circumference of the sample thermostat. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 39 ➔ If your module was shipped in cold weather, leave it in its box and allow it to warm slowly to room temperature to avoid condensation. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 40: Physical Specifications
Boiling point ≥56 °C If a sample cooler or thermostat is included the upper value for humidity can be reduced. Please check your lab conditions to stay beyond dew point values for non–condensing operation. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 41: Performance Specifications
Instrument Control Framework (ICF) A.02.03 or refer to the compatibility matrix of your version above of the LC and CE Drivers Instant Pilot (G4208A) with firmware B.02.19 or above Lab Advisor B.02.07 or above Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 42 GLP features Early maintenance feedback (EMF) for continuous tracking of instrument usage with user-settable limits and feedback messages. Electronic records of maintenance and errors. Housing All materials recyclable. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 43: Performance Specifications (G7129B)
LC and CE Drivers Instant Pilot (G4208A) with firmware B.02.19 or above Lab Advisor B.02.07 or above Communications Controller-area network (CAN),Local Area Network (LAN) ERI: ready, start, stop and shut-down signals Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 44 GLP features Early maintenance feedback (EMF) for continuous tracking of instrument usage with user-settable limits and feedback messages. Electronic records of maintenance and errors. Housing All materials recyclable. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 45: Performance Specifications (G7129C)
Extensive diagnostics, error detection and safety-related display with Agilent Lab Advisor software features Leak detection, safe leak handling, leak output signal for shutdown of pumping system, and low voltages in major maintenance areas Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 46 Performance Specifications 1260 Infinity II Vialsampler (G7129C) Type Specification Comment GLP features Early maintenance feedback (EMF) for continuous tracking of instrument usage with user-settable limits and feedback messages. Electronic records of maintenance and errors. Housing All materials recyclable. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 47: Specifications Of The Sample Cooler
Up to 4600 m (15091 ft) Safety standards: Installation category II, Pollution For indoor use only. IEC, EN, CSA, UL degree 2 ISM Classification ISM Group 1 Class B According to CISPR 11 Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 48 4 °C to 5 °C below ambient Temperature settable from 4 – 40 °C in 1 ° increments Temperature accuracy (<25 °C, <50 % r.H.) 2 °C to 6 °C at a setpoint of 4 °C Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 49: Specifications Of The Sample Thermostat
Up to 4600 m (15091 ft) altitude Safety standards: Installation category II, Pollution For indoor use only IEC, EN, CSA, UL degree 2 ISM Classification ISM Group 1 Class B According to CISPR 11 Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 50 2 – 6 °C at a setpoint of 4 °C accuracy (<25 °C, <50 % r.H.) Minimum firmware revision for the sample thermostat is D.07.22. N O T E Minimum LC driver revision for the sample thermostat is A.02.14. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 51: Specifications Of The Integrated Column Compartment
Site Requirements and Specifications Specifications of the Integrated Column Compartment Specifications of the Integrated Column Compartment Physical Specifications Agilent InfinityLab LC Series Integrated Column Compartment (G7130A) Table 9 Physical Specifications (G7130A) Type Specification Comment Weight 1.8 kg Dimensions 86.5 x 396 x 106.5 mm...
Page 52 Site Requirements and Specifications Specifications of the Integrated Column Compartment Performance Specifications Agilent InfinityLab LC Series Integrated Column Compartment (G7130A) Table 10 Performance Specifications Agilent InfinityLab LC Series Integrated Column Compartment (G7130A) Type Specification Comments Temperature range 5 °C above ambient to 80 °C...
Page 53: Using The Module
Agilent InfinityLab LC Series Vialsamplers User Manual Using the Module Magnets Turn on/off Status Indicators Exchange Drawers Install the Integrated Column Compartment Install the Column Shelf Install the External Tray Install a Column (ICC) Remove a Column (ICC) Sample Trays...
Page 54: Magnets
Using the Module Magnets Magnets This stack exemplarily shows the magnets' positions in the modules. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 55: Turn On/Off
Using the Module Turn on/off Turn on/off Power switch: On Turn instrument On/Off with the control software. Power switch: Off Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 56 Using the Module Turn on/off Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 57: Status Indicators
5. Resident mode (blinking) - for example during update of main firmware. 6. Bootloader mode (fast blinking). Try to re-boot the module or try a cold-start. Then try a firmware update. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 58: Exchange Drawers
N O T E Install classic drawer 1-50 to the left, classic drawer 51-100 to the right side. Open the front door. Pull the drawer out. Lift the front of the drawer. Lift the drawer out. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 59 Using the Module Exchange Drawers Remove the drawer. Insert the back of the drawer. Align the drawer. Push in the drawer. Close the doors. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 60: Install The Integrated Column Compartment
WA R N I N G Solvents may run into the module if Column Shelf or Integrated Column Compartment are not installed. ➔ Install either Column Shelf or Integrated Column Compartment before starting the module. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 61 Using the Module Install the Integrated Column Compartment Press the levers left and right. Pull out the column shelf. The column shelf is removed. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 62 Install the Integrated Column Compartment Push in the Integrated Column Compartment. Click in the levers. The Integrated Column Compartment is installed. Push the latch of the Integrated Column Compartment to Open the flap. the left. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 63 N O T E Do not overtighten the fitting (maximum torque 2.5 Nm). N O T E The use of capillaries with SL or SX fittings are recommended for a better handling. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 64 Position the column into the Column Holder Clip(s). Route the capillary leading to the system between the latch and the Integrated Column Compartment. N O T E Optionally route the capillary to the side. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 65 Using the Module Install the Integrated Column Compartment Close the flap until the latch is locked. Close the doors. Turn on the module and the pump. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 66: Install The Column Shelf
Solvents may run into the module if Column Shelf or Integrated Column Compartment are not installed. ➔ Install either Column Shelf or Integrated Column Compartment before starting the module. Remove the capillary connection from the integrated Remove the column. column compartment to the module. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 67 Install the Column Shelf Unscrew the right fitting. Remove the right fitting. Open the lever of the InfinityLab Quick Connect Fitting. Unscrew and remove the InfinityLab Quick Connect Fitting. Column removed. Close the integrated column compartment. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 68 Using the Module Install the Column Shelf Press the levers left and right. Pull out the integrated column compartment. The integrated column comparment is removed. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 69 Install the Column Shelf Push in the column shelf. Click in the column shelf to fix it properly. The column shelf is installed. Close the doors. Turn on the module and the pump. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 70: Install The External Tray
For best temperature performance, and if the external tray is not in use, it is best to cover the opening for the external tray with the original parts. Lift the foam with a flathead screwdriver. Remove the foam. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 71 Push out the plastic cover. Insert the external tray. Vent for the external tray is open. Install the disposal tube. Close the door. Configure the External Tray in the online view of the Chromatographic Data System. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 72: Install A Column (Icc)
• Open the doors of the sampler A maximum of two columns can be installed. N O T E Push the latch of the Integrated Column Compartment to Open the flap. the left. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 73 N O T E Do not overtighten the fitting (maximum torque 2.5 Nm). N O T E The use of capillaries with SL or SX fittings are recommended for a better handling. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 74 Position the column into the Column Holder Clip(s). Route the capillary leading to the system between the latch and the Integrated Column Compartment. N O T E Optionally route the capillary to the side. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 75 Using the Module Install a Column (ICC) Close the flap until the latch is locked. Configure Columns for the ICC. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 76: Remove A Column (Icc)
Preparations • Switch off the pump • Switch off the sampler • Open the doors of the sampler Push the latch. Open the flap 90 °. Remove the column. Unscrew the right fitting. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 77 Remove a Column (ICC) Remove the right fitting. Open the lever of the InfinityLab Quick Connect Fitting. Unscrew and remove the InfinityLab Quick Connect Column removed. Fitting. Close the flap until the latch is locked. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 78: Sample Trays
Right-hand Drawer for 18 x 6 mL Vials: P2-A1 to P2-C6 Drawer for 50 x 2 mL Vials Classic Left: Vial 1-50 Drawer for 50 x 2 mL Vials Classic Right: Vial 51-100 External Tray 5-position: 201 – 205 Position Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 79 Numbering of drawer position (right-hand Drawer for 66 x 2 mL Vials) Figure 16 Numbering of drawer position (left-hand Drawer for 18 x 6 mL Vials) Figure 17 Numbering of drawer position (right-hand Drawer for 18 x 6 mL Vials) Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 80 Using the Module Sample Trays Figure 18 Numbering of drawer position (Drawer for 50 x 2 mL Vials Classic) Figure 19 Numbering of tray position (External tray) Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 81: Choice Of Vials And Caps
Crimp Top Vial, 2 mL, amber glass, write-on spot, 100/Pack (silanized) 5182-0567 Crimp Top Vial, 1 mL, polypropylene, wide opening, 100/Pack 5183-4496 Crimp Top Vial, 1 mL, polypropylene, wide opening, 100/Pack (silanized) 9301-0978 crimp top vial, 0.3 mL, polypropylene, wide opening, 1000/pack Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 82 Screw Cap Vial, 2 mL, amber glass, write-on spot, 100/pk 5183-2069 Screw Top Vial, 2 mL, amber glass, write-on spot, 1000/Pack 5183-2072 Screw Top Vial, 2 mL, amber glass, write-on spot, 100/Pack (silanized) Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 83 Screw Cap, red polypropylene, septum (clear PTFE/red rubber), 100/Pack 5182-0720 Screw Cap, blue polypropylene, septum (clear PTFE/silicone), 100/Pack 5182-0721 Screw Cap, green polypropylene, septum (clear PTFE/silicone), 100/Pack 5182-0722 Screw Cap, red polypropylene, septum (clear PTFE/silicone), 100/Pack Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 84: Transporting The Sampler With A Sample Cooler Or Sample Thermostat Installed
Ensure adequate ventilation: typical air exchange of 25 m /h per m of laboratory floor area. ➔ Keep all ventilation openings in the enclosure clear of obstructions. Do not block the openings on the circumference of the sample thermostat. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 85 Advisor online help for more information. Also ensure there is no vial in the gripper arm. • Install the transport protection. • Remove all vials from the drawers. • The condensed water inside of the sample cooler or sample thermostat is removed. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 86: Agilent Local Control Modules
• fast and direct control in front of the instrument • a clear overview of the system status • control functionalities • access to method parameters and sequences • a logbook showing events from the modules • diagnose tests Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 87 • Factory installed software – flat dialog structure, user configurable interface, enhanced sequence engine, for example with wait for baseline stabilization, diagnosis with passed/failed. • GLP – System logbook and module log-books record errors, unusual events and maintenance activities for GLP traceability. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 88 Using the Module Agilent Local Control Modules Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 89: Installation Of The Optional Sample Cooler Or Thermostat
Agilent InfinityLab LC Series Vialsamplers User Manual Installation of the Optional Sample Cooler or Thermostat Damaged Packaging Install the Sample Cooler or Thermostat Using the Sample Cooler or Thermostat in an Infinity II Sampler Control Interface Control Temperature Mode Online Signal Monitor...
Page 90: Damaged Packaging
Damaged Packaging Damaged Packaging If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the instrument may have been damaged during shipment. "Defective on arrival" problems C AU T I O N If there are signs of damage, please do not attempt to install the module.
Page 91: Install The Sample Cooler Or Thermostat
WA R N I N G ➔ When handling, installing and operating the Sample Thermostat, care should be taken to avoid damage to the refrigerant tubing or any part of the Sample Thermostat. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 92 Depending on the ambient conditions in the lab, the amount of condensate can vary from N O T E 200 mL to 2 L per day. Waste containers for the condensate should not be filled to the top. Regularly empty the waste container. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 93 Ensure that the power switch on the front of the module Ensure that the power cable is removed from the is OFF (switch stands out). instrument. Open the 4 screws on the rear of the module. Remove the sample cooler/thermostat mainframe cover. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 94 To avoid damages of the electronics of the module make sure the power cords are unplugged before disconnecting or reconnecting the sampler to the sample cooler/thermostat cables. Connect power cable and signal/data cable. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 95 Fit in the sample cooler or thermostat perfectly. Slide in the whole unit. Use a bubble level to check the leveling of the sampler. N O T E The module should be operated in a proper horizontal position. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 96 Wait at least 30 min before switching on the module. ➔ This allows the refrigerant and system lubrication to reach equilibrium. Connect the power cable to the power connector at the rear of the module. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 97: Using The Sample Cooler Or Thermostat In An Infinity Ii Sampler
Within the autosampler GUI, there are active areas. If the mouse cursor is moved across the icons the cursor will change, see the following figure. ALS: turn on and off ALS configuration ALS Status Sample Cooler/Sample Thermostat Status (on/off) Injection Volume Drawer Configuration EMF Status Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 98: Control Interface
3 degrees. If the system is not in the range of ± 2 °C you will see a yellow bar over the temperature values. Control Interface A right-click into the active area will open a menu to show the Control Interface. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 99: Control
Select On to switch on the cooler/thermostat. Specify the required temperature in the adjacent field. Note that the specified temperature must be at least 5 °C below ambient for proper temperature control. Select Off to switch off the cooler/thermostat. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 100: Temperature Mode
This mode may be used for degradation experiments (this solution is not officially recommended by Agilent) or as a workaround for reporting. Reporting of control parameters will become available with the CDS and ICF. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 101: Online Signal Monitor
The online signal monitor for the chiller is also available and allows you to monitor the WPS1A temperature signals. The Online Plot Window should look like this: If the change button is pressed you can select the Chiller signal WPS1A in the online plot. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 102: Operation Information
This will disappear after the unit comes out of the heating cycle and goes back into normal operation. Shut down of the sample cooler or sample thermostat at low temperatures will build up some condensate inside the autosampler. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 103: Important Information
Ramping up from 4 °C to 30 °C with the thermostat happens slowly. It takes more than 1 h N O T E until the instrument reaches the setpoint. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 104 Installation of the Optional Sample Cooler or Thermostat Using the Sample Cooler or Thermostat in an Infinity II Sampler Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 105: Preparing The Module
Agilent InfinityLab LC Series Vialsamplers User Manual Preparing the Module Leak and Waste Handling Preparing the Module Solvent Information Capillary Color Coding Guide Installing Capillaries Flow Connections to the Vialsampler Setting up the Vialsampler Control Settings Method Parameter Settings Injector Programm Module Configuration View This chapter explains the operational parameters of the module.
Page 106: Leak And Waste Handling
N O T E has been shipped with the solvent cabinet, electronic copies are available in the Agilent Information Center or via the Internet. For details on correct installation, see separate installation documentation. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 107: Preparing The Module
(second choice) if no isopropanol is available To clean the system when Bidistilled water Best solvent to re-dissolve using buffers buffer crystals After a solvent change Bidistilled water Best solvent to re-dissolve buffer crystals Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 108: Solvent Information
HPLC analysis over several decades. These materials exhibit excellent robustness under typical HPLC conditions. For any special condition, please consult the material information section or contact Agilent. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 109 PEEK capillaries keeps the flow path free of steel and ensures pressure stability to at least 600 bar. If in doubt, consult the available literature about the chemical compatibility of PEEK. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 110 • High concentrations of inorganic acids like nitric acid, sulfuric acid and organic solvents especially at higher temperatures (replace, if your chromatography method allows, by phosphoric acid or phosphate buffer which are less corrosive against stainless steel). Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 111 3 %). Slight corrosion is possible with ammonia > 10 %. Diamond-Like Carbon (DLC) Diamond-Like Carbon is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 112 For optimizing the life of the pressure sensor, do not leave HFIP in the chamber when the unit is off. The tubing of the leak sensor is made of PVDF (polyvinylidene fluoride), which is incompatible to the solvent DMF (dimethyl formamide). Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 113 Sapphire, Ruby and Al -based ceramics Sapphire, ruby and ceramics based on aluminum oxide Al are inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 114: Capillary Color Coding Guide
Preparing the Module Capillary Color Coding Guide Capillary Color Coding Guide Figure 20 Syntax for capillary description Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 115: Installing Capillaries
The capillaries mentioned above are examples only. Select a nut that is long enough for the fitting you'll be Slide the nut over the end of the tubing or capillary. using. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 116 If changing from a column or an injection valve to another, the fitting may leak or decrease the quality of the separation by contributing to band broadening. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 117: Flow Connections To The Vialsampler
4 Route the drainage of the washport outlet to the waste container. 5 Prime the wash tubing. 6 Install the capillary from the pump outlet into the port 1 of the injection valve. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 118 7 Install the capillary from port 6 of the injection valve to the ICC or MCT The correct plumbing is shown in the figure below and is attached to the module. 8 Prime and purge the complete sampler. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 119: Setting Up The Vialsampler
Figure 21 ChemStation Method and Run Control After successful load of the OpenLab ChemStation, you should see the module as an active item in the graphical user interface (GUI). Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 120 The needle wash specified in this dialog box is carried out immediately. • Modify • Flow Path: for the sample loops, needle seat, extension loops and metering devices • External Tray • Temperature Mode Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 121 The Vialsampler configuration is done in the online module dashboard context menu, not N O T E in the instrument configuration view. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 122: Control Settings
Preparing the Module Setting up the Vialsampler Control Settings The control settings are available via right click on the active area of the grafical user interface, see Table 12 on page 120. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 123 Temperature section. For additional help and support, highlight the desired area and press the F1 key. A help N O T E screen will open with additional information and documentation about the topic. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 124: Method Parameter Settings
Preparing the Module Setting up the Vialsampler Method Parameter Settings The method parameter settings are available via right click on the active area of the grafical user interface. Figure 23 Method parameter settings Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 125 Posttime to delay the start of the next analysis. A Posttime period can be used to allow the column to equilibrate after changes in solvent composition (for example after gradient elution). Limits: 0.01 through 99999 min or Off. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 126 (in mm) away from its standard position. The Offset function is useful when analyzing very small sample volumes, or when only a specific part of the sample is required, for example, the top layer. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 127 For additional help and support, highlight the desired area in the user interface and press N O T E the F1 key. A help screen will open with additional information and documentation about the topic. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 128: Injector Programm
The following functions are available : • Draw • Eject • Mix • Inject • Move • Wait • Valve • Needle • Wash • Remote • Wait For • Repeat. End Repeat • Comment Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 129: Module Configuration View
N O T E screen will open with additional information and documentation about the topic. The vialsampler configuration is done in the module dashboard context menu, not in the N O T E instrument configuration. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 130 Preparing the Module Setting up the Vialsampler Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 131: Optimizing Performance
Agilent InfinityLab LC Series Vialsamplers User Manual Optimizing Performance Optimization for Lowest Carry-over Using the Automated Needle Wash (Wash Port) Using an Injector Program General Recommendation to Lowest Carry-Over Fast Injection Cycle and Low Delay Volume Overlapped Injection Mode General Recommendations for Fast Injection Cycle Times...
Page 132: Optimization For Lowest Carry-Over
It has a volume of ca. 0.5 mL and the peristaltic pump delivers ca. 5 mL/min, which means the wash port volume is completely refilled with fresh solvent in ca. 6 s. If the wash port is selected, the user can set the length Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 133 3 INJECT Line 1 moves the needle to vial P2-A1 or the flushport. Line 2 draws 5 μL from the current sample vial P1-A1. Line 3 injects the sample (valve switches to main pass). Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 134: Using An Injector Program
Wait (equilibration time - see text above) Valve bypass Wait 0.2 min Valve mainpass (main path) Valve bypass Valve mainpass (main path) Overlapped injection together with additional injection valve switching is not possible. N O T E Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 135: General Recommendation To Lowest Carry-Over
• Clean the needle seat with a tissue and remove all liquid. Again be careful not to risk an injury by the uncovered needle. • Reinstall the safety cover of the sampler. • In Lab Advisor, go to Maintenance > Change Needle and finish the procedure. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 136: Fast Injection Cycle And Low Delay Volume
100 μl instead of 1 μl, increase the injection time by approximately 8 sec. In this case and if the viscosity of the sample allows it, the draw and eject speed of the injection system has to be increased. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 137: General Recommendations For Fast Injection Cycle Times
• Increase of draw and eject speed for large injection volumes • Add at last run a blank, if overlapped injection is used To reduce the injection time, the detector balance has to be set to OFF. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 138: Precise Injection Volume
A DRAW statement in an injector program also uses the draw speed setting which is configured for the autosampler. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 139 An EJECT statement in an injector program also uses the eject speed setting which is configured for the autosampler. A faster eject speed shortens the time required to run the injector program. When using viscous samples, a high eject speed should be avoided. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 140: Choice Of Rotor Seal
It is suitable for application using mobile phases within a pH between 1 and 14. Strong oxidizing acids such as concentrated nitric and sulfuric acids are not compatible N O T E with PEEK. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 141: Troubleshooting And Diagnostics
Agilent InfinityLab LC Series Vialsamplers User Manual Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions Maintenance Functions User Interface Change Needle Change Metering Seal or Piston Park Arm Change Gripper Automatic Referencing Vialsampler Gripper Verification Test Step Commands...
Page 142: Overview Of The Module's Indicators And Test Functions
Tray alignment is required after repair of internal components. The procedure aligns the gripper arm correctly to ensure the positioning of the gripper arm is correct for all vials (see “Automatic Referencing” on page 151). Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 143 The step functions provide the possibility to execute each step of the sampling sequence individually. The step functions are used primarily for troubleshooting, and for verification of correct autosampler operation after repair (see “Step Commands” on page 153). Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 144: Maintenance Functions
Change Gripper: The change gripper function moves the gripper to the front of the autosampler enabling easy access to the gripper release mechanism. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 145: Change Needle
Read carefully the desciption in the Lab Advisor user interface. N O T E Move Down The needle arm moves slowly down. Move Up The needle arm slowly moves up. Back Completes the needle, needle seat procedure. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 146 5 Next positions the needle to the top level. Flap the safety wash port back in its origin position. 6 Reinstall the safety cover and complete the procedure. 7 Select Next to move the needle into the needle seat. 8 Select Back to complete the procedure. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 147: Change Metering Seal Or Piston
212). 3 Select Next to move the piston back to the home position. Read carefully the description in the LabAdvisor user interface. N O T E 4 Select Back to finish the procedure. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 148: Park Arm
Control display. The commands for the control software are: Park Arm moves the gripper arm to the park position. Home moves the gripper arm out of the park position to the home position. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 149 Gripper function to remove the vial. 1 Select Park Arm. 2 When the arm is in the park position, install Transport-Protection (G7129-40050). Then the autosampler is ready for shipment, and can be switched OFF. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 150: Change Gripper
2 Exchange the gripper arm (see “Exchange the Gripper Arm” on page 216). 3 Select Next to move the gripper arm to the home position. 4 Select Back to finish the gripper procedure. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 151: Automatic Referencing
Mount the calibration fixture. Position P1-C6 and P2-F6. Please check help for further explanation. N O T E Next Mount a 2 mL vial or gripper adapter from the calibration tool. Next Perform autoreferencing. Next Remove the gripper adapter. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 152: Vialsampler Gripper Verification Test
Automatic Referencing procedure should be done. Allows you to select a vial position. You can select from positions 1, 11, 39, 56, 66, 101, 111, 139, 156 and 166. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 153: Step Commands
Command can be done more than once (maximum draw volume of 100 µL cannot be exceeded). Use Plunger Home to reset the metering device. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 154 Needle into Seat Lowers the needle arm into the seat. Command also returns the vial to the tray position. Valve Mainpass Switches the injection valve to the mainpass (main path) position. Reset Resets the injector. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 155: Tests
0.5 °Cover a 10 s period) the cooler is turned on and measurement is started. For the test to succeed, three temperature checkpoints must be reached within a specified time. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 156: Troubleshooting
Defective metering-drive motor. Needle Up Needle already in the upper position. Needle already in the upper position. Defective or dirty sensor on the needle station flex board. Sticking needle-arm assembly. Defective needle-drive motor. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 157 Needle already in the upper position. Defective or dirty sensor on the needle station flex board. Sticking needle-arm assembly. Defective needle-drive motor. Valve already in mainpass (main path). Valve not connected. Defective injection valve. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 158: Troubleshooting Guide For The Sample Transport Assembly
• motor overtemp (0 or 2 or 3) • movement failed (0 or 2 or 3) • missing vial Motor 0=X; 1=Z; 2=Theta; 3=Gripper. N O T E Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 159: Intermittent Lock-Ups With Or Without Vial In The Gripper Fingers
(G7129-65800)) or the fusion board ( Fusion Board (G4200-65880)). 7 For both steps (step 5 and 6), you have to do an automatic referencing again to store the correct values in the instrument firmware again. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 160: Poor Alignment
(G7129-65800)) or the fusion board ( Fusion Board (G4200-65880)). 5 For all three steps (step 2, 3, and 4) you have to do an automatic referencing again to store the correct values in the instrument firmware again. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 161: Agilent Lab Advisor Software
The optional Agilent Maintenance Wizard Add-on provides an easy-to-use, step-by-step multimedia guide for performing preventive maintenance on Agilent 1200 Infinity and Agilent InfinityLab LC Series instrument. The tests and diagnostic features that are provided by the Agilent Lab Advisor software may differ from the descriptions in this manual. For details, refer to the Agilent Lab Advisor software help files.
Page 162 Troubleshooting and Diagnostics Agilent Lab Advisor Software Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 163: Error Information
Agilent InfinityLab LC Series Vialsamplers User Manual Error Information What are Error Messages General Error Messages Timeout Shutdown Remote Timeout Lost CAN Partner Leak Sensor Leak Sensor Open Leak Sensor Short Compensation Sensor Open Compensation Sensor Short Fan Failed Sampler Error Messages...
Page 164 ICC Heater Temp Sensor Failure ICC Heater Over Temp Failure This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 165: What Are Error Messages
In all cases, error propagation is done via the CAN bus or via an APG/ERI remote cable (see documentation for the APG/ERI interface). Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 166: General Error Messages
Check the vacuum degasser for an error The degasser failed to generate sufficient condition. Refer to the Service Manual for the vacuum for solvent degassing. degasser or the pump that has the degasser built-in. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 167: Remote Timeout
• Ensure all CAN cables are installed correctly. Exchange the CAN cable. Defective CAN cable. Switch off the system. Restart the system, and Defective main board in another module. determine which module or modules are not recognized by the system. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 168: Leak Sensor
• Make sure the leak sensor is thoroughly dry before restarting the autosampler. Exchange the peristaltic pump. Leaking peristaltic pump The leak sensor in the sampler is hidden under the ICC Column Heater or Column Shelf N O T E respectively. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 169: Leak Sensor Open
Please contact your Agilent service representative. Leak sensor incorrectly routed, being pinched by a metal component. Please contact your Agilent service representative. Power switch assembly defective Please contact your Agilent service representative. Cable or contact problem. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 170: Compensation Sensor Open
Probable cause Suggested actions Please contact your Agilent service Defective power switch assembly representative. Please contact your Agilent service Loose connection between the power representative. switch board and the main board Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 171: Fan Failed
Fan cable disconnected. representative. Please contact your Agilent service Defective fan. representative. Replace the sample cooler or sample Defective sample cooler or sample thermostat. thermostat fan Please contact your Agilent service Defective main board. representative. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 172: Sampler Error Messages
If the valve fails to reach the mainpass (main path) position, or if the microswitch does not close, the error message is generated. Probable cause Suggested actions Please contact your Agilent service Defective injection valve. representative. Please contact your Agilent service Defective main board. representative. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 173: Needle Movement Failed
Align gripper. Incorrect gripper alignment. Exchange the gripper assembly. Defective gripper assembly (defective gripper fingers or belt). Please contact your Agilent service representative. Defective transport assembly flex board. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 174: Initialization Failed
Defective needle station board. representative. Please contact your Agilent service Defective transport assembly flex board. representative. Please contact your Agilent service Defective hydraulic unit motor. representative. Please contact your Agilent service Defective main board. representative. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 175: Metering Home Failed
Exchange the metering piston and seal. Broken piston. Please contact your Agilent service Defective metering-drive motor. representative. Please contact your Agilent service Defective main board. representative. Please contact your Agilent service Loose belt. representative. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 176: Motor Temperature
Motor belt tension too high. switch. Wait at least 10 min before switching on again. Please contact your Agilent service Defective motor. representative. Please contact your Agilent service Defective transport assembly flex board. representative. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 177: Initialization With Vial
Probable cause Suggested actions Remove the vial using the Release Vial Vial still in gripper. function in the user interface. Reinitialize the autosampler. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 178: Missing Wash Vial
Tray recognition defective (dirty sample tray are clean (located at the rear of the sample or defective transport assembly flex board). tray). If this does not solve the problem, contact your Agilent service representative. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 179: Valve Switch Not Found
Please contact your Agilent service Rear wheel in the hydraulic unit is loose. representative. Please contact your Agilent service Defective metering-drive motor. representative. Please contact your Agilent service Defective metering device representative. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 180: Sample Cooler Error Messages
Probable cause Suggested actions • Check the power cable from the ALS to the Blown fuses, defective cable sample cooler. • Please contact your Agilent service representative. Replace the sample cooler. Defective PCB boards Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 181: Cooler Temperature Below Or Above Target Temperatur
Probable cause Suggested actions Switch off the instrument and wait for 15 min. Overpressure in the chiller Restart the instrument and check the signals in Lab Advisor. Replace the sample cooler. Defective compressor Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 182: Cooler Condensate Error
There should be no kinked and sealed tubing. Switch the sample cooler off/on and check if Defective condensate sensor after 8 min the error shows up again. Replace the sample cooler. Defective leak sensor Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 183: Integrated Column Compartment (Icc) Heater Error Messages
For safety reason heater over-current protection became active. Probable cause Suggested actions Please contact your Agilent service Defective cable or connector representative. Replace the ICC heater. Defective ICC heater Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 184: Icc Heater Temp Sensor Failure
100 °C. This exceeds the limit of the heating element. Probable cause Suggested actions Please contact your Agilent service Defective cable or connector representative. Replace the ICC heater. Defective ICC heater Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 185: Maintenance And Repair
Agilent InfinityLab LC Series Vialsamplers User Manual Maintenance and Repair Introduction to Maintenance Warnings and Cautions Overview of Maintenance Cleaning the Module Remove and Install Doors Exchange the Needle Assembly Exchange the Needle Seat Assembly Exchange the Sample Loop Assembly...
Page 186: Introduction To Maintenance
The module is designed for easy maintenance. Maintenance can be done from the front with module in place in the system stack. Figure 25 Overview of the Vialsampler There are no serviceable parts inside. N O T E Do not open the module. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 187: Warnings And Cautions
Only certified persons are authorized to carry out repairs inside the module. Sharp metal edges WA R N I N G Sharp-edged parts of the equipment may cause injuries. ➔ To prevent personal injury, be careful when getting in contact with sharp metal areas. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 188 Avoid back strain or injury by following all precautions for lifting heavy objects. ➔ Ensure that the load is as close to your body as possible. ➔ Ensure that you can cope with the weight of your load. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 189: Overview Of Maintenance
Table 17 Overview of maintenance Procedure Typical interval (minimum) Notes Change 30000 needle into seat movements needle/needle seat Change peristaltic 3000 min on time pump cartridge Change rotor seal 30000 injections Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 190: Cleaning The Module
WA R N I N G hazard and damage the module ➔ Do not use an excessively damp cloth during cleaning. ➔ Drain all solvent lines before opening any connections in the flow path. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 191: Remove And Install Doors
Press the release buttons and pull the front door out. For the Installation of the front door, insert the hinges into their guides and move the door in until the release buttons click into their final position. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 192: Exchange The Needle Assembly
Wear safety goggles and safety gloves when removing the needle assembly. It is recommended to always exchange the needle assembly and the needle seat at the N O T E same time to prevent premature leakage. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 193 Do not touch the tip of the needle. Do not change the needle seat at this point. Remove the safety cover. ➔ Do not touch the tip of the needle. Turn or flap the wash port to the left. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 194 Lift out the needle. Insert the needle (1). Align the needle in the seat, then Align the needle in the seat (needle may be bent slightly tighten the screw firmly (2). manually, if not aligned properly). Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 195 Incorrect positioning and installation of the needle/loop connection can result in damaging and breaking the sample loop. Turn or flap the wash port to the right. Install the safety cover (1.) and fix the screw (2.). Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 196 Maintenance and Repair Exchange the Needle Assembly Select Next to move the needle into the needle seat. Close the doors. Select Back to finish the procedure. Perform a pressure test. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 197: Exchange The Needle Seat Assembly
Wear safety goggles and safety gloves when removing the needle assembly. When the instrument setup has changed, configure the new setup in the online view of N O T E the Chromatographic Data System. See Table 12 on page 120. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 198 Do not touch the tip of the needle. Do not change the needle seat at this point. Remove the safety cover. ➔ Do not touch the tip of the needle. Turn or flap the wash port to the left. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 199 2 mm above the seat. Loosen the fixing screw. Lift out the needle. Disconnect the seat-capillary fitting from the injection Use a small flat-head screwdriver to ease out the needle valve (port 5). seat. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 200 (2). N O T E Do not overtighten the fitting! N O T E Incorrect positioning and installation of the needle/loop connection can result in damaging and breaking the sample loop. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 201 Install the safety cover (1.) and fix the screw (2.). Select Next to move the needle into the needle seat. Select Back to finish the procedure. Close the doors. Perform a pressure test. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 202: Exchange The Sample Loop Assembly
Wear safety goggles and safety gloves when removing the needle assembly. When the instrument setup has changed, configure the new setup in the online view of N O T E the Chromatographic Data System. See Table 12 on page 120. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 203 Check if the needle is in maintenance position. WA R N I N G Sharp needle Uncovered needles may cause injuries ➔ Do not touch the tip of the needle. Remove the safety cover. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 204 Click on Next in the LabAdvisor Needle lift up again. tip position by using the Needle Control function in Lab Advisor. Turn or flap the wash port to the right. Install the safety cover (1.) and fix the screw (2.). Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 205 Maintenance and Repair Exchange the Sample Loop Assembly Click Next until the needle assembly is moved to the Close the doors. needle seat. Select Back to finish the procedure. Perform a pressure test. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 206: Exchange The Rotor Seal
The stator face is held in place by the stator head. When you remove the stator head, the stator face can fall out of the valve. ➔ Carefully handle the valve to prevent damage to the stator face. ➔ Carefully handle the stator face during sonication. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 207 Remove all capillaries from the injection valve with a 1/4 inch wrench. Loosen each fixing bolt two turns at a time. Remove the Remove the stator head and stator ring. bolts from the head. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 208 N O T E Make sure that the rotor sealing surface with its engraved flow passages is facing out. The pattern is asymmetrical to prevent improper placement. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 209 Do not over-tighten the screws. The screws hold the assembly together and do not affect the sealing force. The sealing force is automatically set as the screws close the stator head against the valve body. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 210 Reconnect the capillaries and tubes to the valve ports. Slide the waste tube into the waste holder in the leak tray. The correct plumbing is shown below and on the safety cover of the module. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 211 Maintenance and Repair Exchange the Rotor Seal Close the doors. Perform a pressure test. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 212: Exchange The Metering Seal And Piston
Finish any pending acquisition job and in order to avoid leaks, stop the pump running. When the instrument setup has changed, configure the new setup in the online view of N O T E the Chromatographic Data System. See Table 12 on page 120. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 213 Open the front door. Remove the two fixing bolts. Pull the metering head assembly away from the sampler. Remove the two fixing bolts from the base of the Disassemble the metering head assembly. metering head assembly. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 214 Tighten the bolts alternately two turns at a time until the metering head must be on the same side as the lower stator head is secure. The housing must be flush with one of the two capillary drillings. the metering head. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 215 Service & Diagnostics > Maintenance Positions > Change Metering Device: click Next and wait until the metering device is in Home position. Select Back to finish the procedure. Perform a pressure test. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 216: Exchange The Gripper Arm
In the Tools section of the Agilent Lab Advisor software select Service & Diagnostics > Maintenance Positions > Change Gripper, click Start and wait until the gripper is in maintenance position. Open the front door. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 217 In the Agilent Lab Advisor software select Change gripper > Next, click Next and wait until the gripper is in the default position. Select Back to finish the procedure. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 218 Maintenance and Repair Exchange the Gripper Arm Close the doors. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 219: Replace The Peristaltic Pump Cartridge
The peristaltic pump cartridge is a replaceable unit. The tubing inside the pump is not N O T E replaceable. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 220 Push the new cartridge onto the motor shaft until the Connect the wash port tubing to the upper tubing of the clips click into place. new cartridge (use sand paper to get a good grip on the tubing). Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 221 Connect the inlet filter of the solvent bottle. Use the Close the doors. syringe to draw enough solvent for completely filling of the peristaltic pump tubing before continuing to prime the peristaltic pump. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 222: Exchange The Wash Port Assembly
In the Agilent Lab Advisor software select Service & Diagnostics in the system screen (Tools) Maintenance Positions > Change Needle/Loop, click Start and wait until the needle assembly is in maintenance position. Open the front door. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 223 ➔ Do not touch the tip of the needle. Turn or flap the wash port to the left. The needle will move slowly downwards and will stop approximately 2 mm above the seat. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 224 Loosen the fixing screw. Lift out the needle. Disconnect the wash tubing from the peristaltic pump. Release the spring loaded pin. Lift the flapper out of the bracket. Be careful not to squeeze the wash tubing. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 225 Install and reconnect the wash tubing to the peristaltic Insert the needle (1). Align the needle in the seat, then pump. tighten the screw firmly (2). Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 226 Incorrect positioning and installation of the needle/loop connection can result in damaging and breaking the sample loop. Turn or flap the wash port to the right. Install the safety cover (1.) and fix the screw (2.). Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 227 Select Next to move the needle into the needle seat. Close the doors. Select Back to finish the procedure. Purge the wash port. Check if solvent will be delivered from the peristaltic pump. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 228: Replace The Module Firmware
1 Download the required module firmware, the latest FW Update Tool and the documentation from the Agilent web. http://www.agilent.com/en-us/firmwareDownload?whid=69761 2 For loading the firmware into the module follow the instructions in the documentation. Module Specific Information There is no specific information for this module. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 229: Parts And Materials For Maintenance
Agilent InfinityLab LC Series Vialsamplers User Manual Parts and Materials for Maintenance Main Assemblies Standard Parts Drawer Assembly External Tray Analytical Head Assembly (40 μL) Analytical Head Assembly (100 µL) 2ps 6pt Injection Valve 1300 bar 2ps 6pt Injection Valve 800 bar...
Page 230: Main Assemblies
2pos/6port Injection Valve 1300 bar 5067-4245 2pos/6port Injection Valve 600 bar 5067-6689 2pos/6port RC Injection Valve 800 bar 5065-4445 Peristaltic pump with Pharmed tubing G1312-87303 Capillary ST 0.17 mm x 400 mm S/S Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 231 Parts and Materials for Maintenance Main Assemblies Figure 26 Main assemblies Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 232: Standard Parts
Sample Loop 20 µL (red coded) G7129-60400 Sample Loop 40 µL (green coded) G7129-60500 Sample Loop 100 µL (white coded) 5065-4445 Peristaltic pump with Pharmed tubing 5063-6506 Finger caps (x3) Reorder gives pack of 15 Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 233: Drawer Assembly
Drawer for 50 x 2 mL Vials Classic Right G7129-68210 Drawer-Kit 100 x 2 mL Vials Classic Figure 27 Drawer for 66 x 2.0 mL Vials Figure 28 Drawer for 18 x 6 mL Vials Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 234 It is required that all drawers are put into correct positions, that is, classical drawer 1-50 N O T E must be on the left side and classical drawer 51-100 on the right side. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 235: External Tray
Parts and Materials for Maintenance External Tray External Tray Description G7129-60000 External Tray for 5 x 2 mL Vials G1313-27302 Disposal tube Figure 30 External tray Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 236: Analytical Head Assembly (40 Μl)
Analytical-Head 40 µL 0905-1717 Metering seal, 40 µL G7129-60184 Seal Support Assembly 40 µL 0515-1052 Screw 2.5 mm hex G7129-60006 Adapter-Assembly Analytical-Head 0515-0850 Screws 5067-5920 Piston ceramic Figure 31 Analytical head assembly (40 µL) Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 237: Analytical Head Assembly (100 Μl)
Analytical-Head 100 µL 0905-1503 Metering seal, 100 µL G7129-60182 Seal Support Assembly 100 µL 0515-1052 Screw 2.5 mm hex G7129-60006 Adapter-Assembly Analytical-Head 0515-0850 Screws 5067-5678 Piston ceramic Figure 32 Analytical head assembly, 100 µL Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 238: 2Ps 6Pt Injection Valve 1300 Bar
2pos/6port Injection Valve 1300 bar 5068-0018 Stator screws 5068-0216 Stator, Injection Valve, 1300 bar 5068-0118 Stator ring 5068-0007 Rotor seal (Vespel), 1300 bar 1535-4045 Bearing ring Figure 33 2ps 6pt Injection Valve 1300 bar Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 239: 2Ps 6Pt Injection Valve 800 Bar
2pos/6port RC Injection Valve 800 bar 5068-0018 Stator screws 5068-0215 Stator, Injection Valve, 600 bar/800 bar 5068-0118 Stator ring 0101-1416 Rotor seal (PEEK) 1535-4045 Bearing ring Figure 34 2ps 6pt Injection Valve 600 bar/800 bar Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 240: 2Ps 6Pt Injection Valve 600 Bar
2pos/6port Injection Valve 600 bar 5068-0018 Stator screws 5068-0215 Stator, Injection Valve, 600 bar/800 bar 5068-0118 Stator ring 0101-1416 Rotor seal (PEEK) 1535-4045 Bearing ring Figure 35 2ps 6pt Injection Valve 600 bar/800 bar Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 241: Integrated Column Compartment
Integrated Column Compartment Integrated Column Compartment Description G7130-60030 ICC Column Heater 3 µL volume G7130-60060 ICC Column Heater 6 µL volume 5043-1356 Colum Holder Lamella G7129-60057 Shelf Assembly (not shown) Figure 36 Integrated column compartment Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 242: Cabinet Kit
Cabinet Kit Item Description G7129-68713 Cabinet Kit Side Cover right Base Cover Side Cover left Top cover G7129-60057 Shelf Assembly G7129-40008 Rail left (not shown) G7129-40009 Rail right (not shown) G1313-22406 SCREW-FIX (not shown) Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 243 Parts and Materials for Maintenance Cabinet Kit Figure 37 Cabinet kit Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 244: Sample Thermostat
The Sample Thermostat Upgrade (G4761A) contains: Item Description G7167-60101 InfinityLab Sample Thermostat G4267-81015 Cable Power Sample Cooler not shown G4267-81014 Cable-Ribbon Sample Cooler not shown 2110-1519 Fuse 3.50 A125 V not shown 5067-6208 Condensate Drainage Kit not shown Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 245 Parts and Materials for Maintenance Sample Thermostat Figure 38 Sample cooler The Sample Thermostat contains flammable refrigerant R600a. Please check further N O T E details for installation. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 246: Standard Vialsampler Accessory Kit
Tube Connector, 90 degree, ID 6.4 5063-6527 Tubing assembly, i.d. 6 mm, o.d. 9 mm, 1.2 m (to waste) 5181-1519 CAN cable, Agilent module to module, 1 m 5067-5967 Tubing Clip Tube Connector (not shown) Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 247 Parts and Materials for Maintenance Standard Vialsampler Accessory Kit Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 248: Multi-Draw Kit
Union ZDV The internal volume of the seat capillary is 400 µL or 1400 µL. With the sample loop the N O T E maximum draw volume of 3600 µL can be reached. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 249: Additional Part List
Parts and Materials for Maintenance Additional Part List Additional Part List Description G7129-68001 Insulation Kit G7129-68000 Air guide kit G7129-60014 Calibration Tool (including gripper adapter) 5023-2540 Adapter ICC Diffusor G7129-40050 Transport-Protection Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 250: Column Identifier Kit 2C (G4751A)
Column Identifier Kit Dual 5067-5917 Custum column ID TAG The InfinityLab Column Identification Tags used in G7116A/B and G7129A/B/C are not N O T E compatible with the former Column Identification Tags used in G1316x. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 251: Identifying Cables
Agilent InfinityLab LC Series Vialsamplers User Manual Identifying Cables Cable Overview Analog Cables Remote Cables CAN/LAN Cables RS-232 Cables This chapter provides information on cables used with the Agilent 1200 Infinity Series modules. Agilent Technologies...
Page 252: Cable Overview
Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent 35900A A/D converter...
Page 253 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m USB cables Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 254: Analog Cables
The other end depends on the instrument to which connection is being made. Agilent Module to 35900 A/D converters p/n 35900-60750 35900 Pin Agilent Signal Name module Not connected Shield Analog - Center Analog + Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 255 Pin BNC Pin Agilent Signal Name module Shield Shield Analog - Center Center Analog + Agilent Module to General Purpose p/n 01046-60105 Pin Agilent Signal Name module Not connected Black Analog - Analog + Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 256: Remote Cables
POWER ON High grey NOT USED pink SHUT DOWN blue START PREPARE black 1wire DATA violet DGND grey-pink +5V ERI out red-blue PGND white-green PGND brown-green +24V ERI out white-yellow +24V ERI out yellow-brown Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 257 • 5188-8045 ERI to APG (Connector D_Subminiature 15 pin (ERI), Connector D_Subminiature 9 pin (APG)) p/n 5188-8045 Pin (ERI) Signal Pin (APG) Active (TTL) Start Request Stop Ready High Power on High Future Shut Down Start Prepare Ground Cable Shielding Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 258 Ground Cable Shielding One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 259 Wire Color Pin Agilent Signal Name Active module (TTL) White Digital ground Brown Prepare run Gray Start Blue Shut down Pink connected Yellow Power on High Ready High Green Stop Black Start request Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 260: Can/Lan Cables
CAN cable, Agilent module to module, 1 m LAN Cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection) Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 261: Rs-232 Cables
It's also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 262: Usb
To connect a USB Flash Drive use a USB OTG cable with Mini-B plug and A socket. Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 263: Hardware Information
Agilent InfinityLab LC Series Vialsamplers User Manual Hardware Information Firmware Description Electrical Connections Rear View of the Module Serial Number Information Interfaces Overview Interfaces ERI (Enhanced Remote Interface) USB (Universal Serial Bus) Setting the 6-bit Configuration Switch Special Settings Instrument Layout...
Page 264: Firmware Description
• run synchronization through APG/ERI remote, • error handling, • diagnostic functions, • or module specific functions like • internal events such as lamp control, filter movements, • raw data collection and conversion to absorbance. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 265 Update of main system can be done in the resident system only. Update of the resident N O T E system can be done in the main system only. Main and resident firmware must be from the same set. Figure 39 Firmware Update Mechanism Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 266 All this specific information is described in the documentation provided with the firmware update tools. The firmware update tools, firmware and documentation are available from the Agilent web. • http://www.agilent.com/en-us/firmwareDownload?whid=69761 Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 267: Electrical Connections
There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply. Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations.
Page 268: Rear View Of The Module
• CN = China Alphabetic character A-Z (used by manufacturing) Alpha-numeric code 0-9, A-Z, where each combination unambiguously denotes a module (there can be more than one code for the same module) 00000 Serial number Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 269: Interfaces
Hardware Information Interfaces Interfaces The Agilent InfinityLab LC Series modules provide the following interfaces: Table 19 Agilent InfinityLab LC Series Interfaces Module RS-232 Analog APG (A) Special (on-board) / ERI (E) Pumps G7104A/C G7110B G7111A/B, G5654A G7112B G7120A G7161A/B Samplers...
Page 270 • RS-232C as interface to a computer • USB (Universal Series Bus) as interface to a computer • REMOTE connector as interface to other Agilent products • Analog output connector(s) for signal output Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 271: Overview Interfaces
For details on USB refer to “USB (Universal Serial Bus)” on page 276. Analog Signal Output The analog signal output can be distributed to a recording device. For details refer to the description of the module’s main board. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 272 Remote (ERI) The ERI (Enhanced Remote Interface) connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. It allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
Page 273 PREPARE (L) Request to prepare for analysis (for example, calibration, detector lamp on). Receiver is any module performing pre-analysis activities. Special Interfaces There is no special interface for this module. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 274: Eri (Enhanced Remote Interface)
ERI replaces the AGP Remote Interface that is used in the HP 1090/1040/1050/1100 HPLC systems and Agilent 1100/1200/1200 Infinity HPLC modules. All new InfinityLab LC Series products using the FUSION core electronics use ERI. This interface is already used in the Agilent Universal...
Page 275 This serial line can be used to read out an EPROM or write into an EPROM of a connected ERI-device. The firmware can detect the connected type of device automatically and update information in the device (if required). Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 276: Usb (Universal Serial Bus)
• Short circuit will be detected through hardware. USB (Universal Serial Bus) USB (Universal Serial Bus) - replaces RS232, supports: • a PC with control software (for example Agilent Lab Advisor) • USB Flash Disk Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 277: Setting The 6-Bit Configuration Switch
• For specific LAN modes switches 4-5 must be set as required. • For boot resident/cold start modes switches 1+2 or 6 must be UP. Figure 42 Location of Configuration switch (example shows a G7114A/B VWD) Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 278 When selecting mode COM, settings are stored to non-volatile memory. When selecting mode TEST, COM settings are tak- en from non-volatile memory. not assigned - Always keep these switches on position ‘0’ (off) Default IP Address is 192.168.254.11 Host Name will be the MAC address. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 279: Special Settings
Exceptions are calibration settings, diagnosis and repair log books which will not be erased. ➔ Save your methods and data before executing a forced cold start. If you use the following switch settings and power the instrument up again, it will start as described above. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 280 Boot Main System / Keep Data Boot Resident System / Keep Data Boot Main System / Revert to Default Data Boot Resident System / Revert to Default Data Note: The setting '0' (down) is essential. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 281: Instrument Layout
• the plastic layers help cushion the electronic and mechanical parts from physical shock, and • the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 282: Early Maintenance Feedback (Emf)
EMF limits, and then reset the EMF counters to zero. The next time the EMF counters exceed the new EMF limits, the EMF flag will be displayed, providing a reminder that maintenance needs to be scheduled. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 283 Agilent InfinityLab LC Series Vialsamplers User Manual LAN Configuration What You Have to Do First TCP/IP parameter configuration Configuration Switches Initialization Mode Selection Dynamic Host Configuration Protocol (DHCP) General Information (DHCP) Setup (DHCP) Manual Configuration With Telnet PC and Agilent ChemStation Setup...
Page 284: Lan Configuration What You Have To Do First
284) to • the PC network card using a crossover network cable (point-to-point) or • a hub or switch using a standard LAN cable. Figure 44 Location of LAN interfaces and MAC label Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 285: Tcp/Ip Parameter Configuration
(short form ‘init mode’) defines how to determine the active TCP/IP parameters after power-on. The parameters may be derived non-volatile memory or initialized with known default values. The initialization mode is selected by the configuration switch, see Table 23 page 287. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 286: Configuration Switches
Location of Configuration switch (example shows a G7114A/B VWD) The module is shipped with all switches set to OFF, as shown above. To perform any LAN configuration, SW1 and SW2 must be set to OFF. N O T E Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 287: Initialization Mode Selection
When initialization mode Using Stored is selected, the parameters are taken from the non-volatile memory of the module. The TCP/IP connection will be established using these parameters. The parameters were configured previously by one of the described methods. Figure 46 Using Stored (Principle) Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 288 In the Using Default mode, the parameters stored in the memory of the module are not N O T E cleared automatically. If not changed by the user, they are still available, when switching back to the mode Using Stored. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 289: Dynamic Host Configuration Protocol (Dhcp)
2 It may be necessary to fully qualify the hostname with the DNS suffix, e.g. 0030d3177321.country.company.com. 3 The DHCP server may reject the hostname proposed by the card and assign a name following local naming conventions. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 290: Setup (Dhcp)
0030d3177321. On the Local Controller the MAC address can be found under Details in the LAN section. Figure 49 LAN Setting on Instant Pilot Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 291 Lab Advisor, Firmware Update Tool) and use MAC address as host name, e.g. 0030d3177321. The LC system should become visible in the control software (see Note in section “General Information (DHCP)” on page 289). Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 292: Manual Configuration
Therefore, manual configuration can be done at any time. A power cycle is mandatory to make the stored parameters become the active parameters, given that the initialization mode selection switches are allowing Figure 50 Manual Configuration (Principle) Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 293: With Telnet
Handheld Controller, or the default IP address (see “Configuration Switches” on page 286). When the connection was established successfully, the module responds with the following: Figure 52 A connection to the module is made Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 294 Then press [Enter], where parameter refers to the configuration parameter you are defining, and value refers to the definitions you are assigning to that parameter. Each parameter entry is followed by a carriage return. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 295 Initialization mode is Using Stored active TCP/IP settings stored TCP/IP settings in non-volatile memory connected to PC with controller software (e.g. Agilent ChemStation), here not connected Figure 55 Telnet - Change IP settings Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 296 If the Initialization Mode Switch is changed now to “Using Stored” mode, the instrument N O T E will take the stored settings when the module is re-booted. In the example above it would be 192.168.254.12. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 297: Pc And Agilent Chemstation Setup
This procedure describes the change of the TCP/IP settings on your PC to match the module’s default parameters in a local configuration (see Table 24 on page 288). 1 Open the Local Area Connection Properties and select Internet Protocol (TCP/IP). Then click on Properties. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 298 LAN Configuration PC and Agilent ChemStation Setup 2 You may enter here the fixed IP address of the module or use the Alternative Configuration. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 299 LAN Configuration PC and Agilent ChemStation Setup 3 We will use the direct LAN access via Cross-over LAN cable with the module’s IP address. 4 Click on OK to save the configuration. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 300: Agilent Chemstation Setup
The LAN must be connected to detector due to high data load on communication to N O T E Control Software. 1 Open the ChemStation Configuration Editor. 2 Select from the menu Configure - Instruments. 3 Select Modular 3D LC System. 4 Give the Instrument a name. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 301 LAN Configuration PC and Agilent ChemStation Setup 5 Click on OK. 6 Select LC System Access — Access Point and click on Add. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 302 9 Save the current configuration via File – Save. 10 Exit the Configuration Editor. 11 Start the Agilent ChemStation. During first startup or when the system configuration has changed, a notification shows up. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 303 13 Enter the IP address or the Hostname of the module with the LAN-access. 14 Click on OK. The selected module is shown now in the right window (with serial number). In addition all other modules connected via CAN to the detector are shown as well. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 304 16 You may see the details of the module by selecting the module and clicking on Configure. Under Connection Settings you may change the IP/Hostname of the module (may require a re-start of the ChemStation). Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 305 PC and Agilent ChemStation Setup After successful load of the ChemStation, you should see the module(s) as active item in the graphical user interface (GUI). Figure 57 Screen After Successful Load of ChemStation Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 306 LAN Configuration PC and Agilent ChemStation Setup Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 307: Appendix
Agilent InfinityLab LC Series Vialsamplers User Manual Appendix General Safety Information General Safety Information Safety Standards General Before Applying Power Ground the Instrument Do Not Operate in an Explosive Atmosphere Do Not Remove the Instrument Cover Do Not Modify the Instrument...
Page 308: General Safety Information
Do not use this product in any manner not specified by the manufacturer. The protective features of this product may be impaired if it is used in a manner not specified in the operation instructions. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 309: Before Applying Power
Verify that the voltage range and frequency of your power distribution matches to the power specification of the individual instrument. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. ➔...
Page 310: Do Not Operate In An Explosive Atmosphere
WA R N I N G Personal injury (for example electrical shock, intoxication) ➔ Instruments that appear damaged or defective should be made inoperative and secured against unintended operation until they can be repaired by qualified service personnel. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 311: Solvents
For details, see the usage guideline for the solvent cabinet. A printed copy of the guideline N O T E has been shipped with the solvent cabinet, electronic copies are available in the Agilent Information Center or via the Internet. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 312: Symbols
European Community directives. The European Declaration of Conformity is available at: http://regulations.corporate.agilent.com/DoC/search.htm Manufacturing date. Power symbol indicates On/Off. The apparatus is not completely disconnected from the mains supply when the power switch is in the Off position Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 313 C AU T I O N alerts you to situations that could cause loss of data, or damage of equipment. ➔ Do not proceed beyond a caution until you have fully understood and met the indicated conditions. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 314: Waste Electrical And Electronic Equipment (Wee) Directive
Do not dispose of in domestic household waste N O T E To return unwanted products, contact your local Agilent office, or see http://www.agilent.com for more information. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 315: Refrigerant
101.1 °C Critical pressure 40.6 bar Boiling point -26.5 °C Table 30 Physical properties of refrigerant R600a (isobutane) Molecular weight 58.12 Critical temperature 134.98 °C Critical pressure 36.6 bar Boiling point -11.7 °C Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 316 All media must be disposed of in accordance with national and local regulations. ➔ Please contact your local Agilent Service Center in regard to safe environmental disposal of the appliance or check www.agilent.com for more info. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 317 Dispose of properly in accordance with federal or local regulations. Flammable Refrigerant Used. ➔ Do not dispose of in domestic household waste. ➔ To return unwanted products, contact your local Agilent office, or see http://www.agilent.com for more information. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 318: Radio Interference
Appendix Radio Interference Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with equipment unscreened...
Page 319: Sound Emission
This product has a sound pressure emission (at the operator position) < 70 dB. • Sound Pressure Lp < 70 dB (A) • At Operator Position • Normal Operation • According to ISO 7779:1988/EN 27779/1991 (Type Test) Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 320: Solvent Information
(e.g. EDTA), • mixtures of carbon tetrachloride with 2-propanol or THF. • Avoid the use of dimethyl formamide (DMF). Polyvinylidene fluoride (PVDF), which is used in leak sensors, is not resistant to DMF. Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 321: Agilent Technologies On Internet
Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 322 191, valve failed capillary connections remove 191, valve switch not found installing draw speed 138, vial in gripper caps DRAW 138, exchange crimp drawer column screw drawers drawers snap Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 323 153, leak sensor leak non-operating altitude injection sequence sensor non-operating temperature injection valve line frequency injection volume precision line voltage Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 324 PC and Agilent ChemStation Setup installation transport PC setup sample drawers troubleshooting local configuration sampler error messages 142, performance specifications transport status indicators peristaltic pump cartridge sampling sequence replace sensor physical specifications leak Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 325 138, voltage range waste electrical and electronic equipment WEEE directive weight X-axis Z-axis Agilent InfinityLab LC Series Vialsamplers User Manual...
Page 326 • optimizing performance, • troubleshooting and diagnostics, • error information, • maintenance, • parts and materials, • hardware information, • LAN configuration, • safety and related information. © Agilent Technologies 2015-2018 Printed in Germany 08/2018 *G7129-90010* *G7129-90010* G7129-90010 Rev. D Agilent Technologies...

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Agilent Technologies InfinityLab LC Series User Manual

Table of Contents

2 Site Requirements and Specifications

3 Using the Module

4 Installation of the Optional Sample Cooler or Thermostat

5 Preparing the Module

6 Optimizing Performance

7 Troubleshooting and Diagnostics

8 Error Information

9 Maintenance and Repair

10 Parts and Materials for Maintenance

12 Hardware Information

13 LAN Configuration

14 Appendix

Quick Links

Chapters

Troubleshooting

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Summary of Contents for Agilent Technologies InfinityLab LC Series