Configuration And Operation Of The Fraction Collector; Delay Volumes And Delay Calibration - Agilent Technologies 1260Infinity II Bio-inert FractionCollector User Manual

3
Using the Module

Configuration and Operation of the Fraction Collector

Configuration and Operation of the Fraction Collector

Delay Volumes and Delay Calibration

Once software is installed and the fraction collector is ready to be operated,
the fraction delay time needs to be determined.
schematic drawing of the flow path between the detector and the fraction
collector with the two delay volumes V
collection the system delay times t
delay volumes by the flow rate.
Figure 4
The delay volume V
fraction collector tubing. Delay volume V
installed Fraction Collector Tubing Kit, is determined using the Delay Volume
Calibration feature of the Lab Advisor software.
32
Delay volumes and delay times
is a system parameter, it depends on the installed
D2
1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual
Figure 4
and V . For peak-based fraction
D1 D2
and t can be calculated by dividing the
D1 D2
, which is specified through the
D1
on page 32 shows a