Uploading Counts - nanoString GeoMx DSP User Manual

MAN-10152-01 for software v2.5

Uploading Counts

Uploading Counts
1. Click on Data Collection then Upload Counts/Cal Files. The Upload Count Data and Cal
Files window opens
2. Click Choose File and navigate to the
zipped counts folder: DCC.zip for NGS
readout or RCC.zip for nCounter readout.
3. If you encounter an error in uploading counts, check these points:
For NGS readout:
Make sure there is not a
l
subfolder within the
DCC.zip folder.
Make sure the DCC file
l
names match the
SampleID names in the
SeqCodeIndices.csv of
the readout package.
Make sure that there is a
l
DCC file for every
sample in the readout
group.
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
(see Figure 54).
For nCounter readout:
Make sure there is not a folder within the RCC.zip folder.
l
Make sure the correct Hyb Code Lot number is
l
associated with the experiment. Check by clicking on the
plate icon.
Make sure the correct CDF was used for the nCounter
l
run.
Make sure SampleID in the CDF matches SampleID and
l
CartridgeID in the RCC files.
nCounter data require a Calibration file for each new lot
l
of Hyb Code. Download lot-specific Calibration files from
http://www.nanostring.com/dspcalibfiles
the instrument (Calibration File Download)
Figure 54).
GeoMx DSP Instrument User Manual
Figure 54: Upload Count Data And Cal Files window
or directly from
(see
65