nanoString GeoMx DSP User Manual page 23

Instrument
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MAN-10152-01 for software v2.5
Start a New Run
3. Place your slide in the slide holder, face up, label toward
slide holder clamp opening
the slide is evenly seated in the slide holder slot.
4. Lower the slide holder clamp
The slide label should be visible in the rectangular
l
window above the green slide slot number.
Tissue may not extend beyond the gasket
l
boundary.
Clean the bottom of the slide again, if needed, with a
l
lint-free wipe and 70% ethanol.
IMPORTANT:
gasket and the slide. Failure to clear material can affect gasket sealing, causing leakage and
instrument damage.
5. Add 6 mL Buffer S onto each slide
Use Buffer S from the Slide Preparation Kit.
IMPORTANT:
shielded from light at all times.
6. Record the location of each slide in the slide holder.
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
(see Figure
9). Ensure that
(see Figure
10).
Ensure that there is no tissue, slide label, or other material between the
(see Figure
Do not leave your slides exposed to air or light. Keep covered with buffer and
GeoMx DSP Instrument User Manual
Figure 9: Loading the slide holder
Figure 10: Lowering the slide holder
clamps
11).
Figure 11: Adding buffer
23

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