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GeoMx DSP Instrument User Manual
Unload Plate and Slides
Removing the Slide Holder
1. Follow the prompts in the GeoMx DSP Control Center to
remove the slide holder from the stage
and move it to a designated laboratory space.
2. Using a pipette, remove and dispose of the buffer from
each slide in the slide holder.
3. Open each slide slot clamp and unload each slide.
4. Store the slides:
Protein slides: Storage up to 1 day - submerge in 1X
l
TBS- T at 4°C, protected from light. Storage >1 day - follow slide mounting procedure,
below.
RNA slides can be stored submerged in 2X SSC at 4°C protected from light for up to 7
l
days. Storage > 7 days is not recommended.
5. Clean the slide holder. See
6. Return the slide holder to the GeoMx instrument.
IMPORTANT:
shutting down or restarting. If the door is locked, click Assist (wrench icon), Restart Data
Collection Workflow to reach the prompt to open the door.
7. Follow the prompts to close the instrument door.
Slide Mounting Procedure for Storage of Protein Slides (>1 day)
1. Rinse slide to be mounted with TBS-T or PBS-T. Touch the slide edge to a paper towel to
remove excess liquid. Place slides on a flat surface.
2. Using a pipette tip (200 µL tip works well), add one drop (~50 µL) of Fluoromount-G to the
slide; add more as necessary to ensure the slide does not dry out and there is adequate tissue
coverage.
3. Mount coverslip (align one edge, then slowly lower from one side to the other) and remove
excess mounting medium.
4. Allow slide to dry at room temperature in the dark overnight (such as bench drawer).
5. Store slide at 4°C, protected from light, for up to 3 months.
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
60
(see Figure 49)
Cleaning the Slide Holder on page
Store the slide tray in the instrument. Ensure it is in the instrument before
MAN-10152-01 for software v2.5
Figure 49: Unloading the slide holder
105.
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