Finalize The Collection Plate - nanoString GeoMx DSP User Manual

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MAN-10152-01 for software v2.5
Finalizing

Finalize the collection plate

When you finalize the collection plate, you are designating the group of samples that will be
processed together in the subsequent readout steps.
Notes on finalizing the collection plate:
Plates are finalized by row, not by the well, even if some wells in the row are unused.
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Once you've finalized a set of rows in a readout group, you cannot add more unfinalized
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rows to that readout group. If you intend to add additional aspirates to a collection plate, do
not finalize the readout group until all aspirates have been collected.
You can later re-insert the plate and use the unused rows:
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• If the previous collection has been finalized, a different readout group must be
used. Collection will begin in the next available row of the plate.
• If the previous collection has not been finalized, the same readout group must be
used. Collection will begin in the next available well of the plate, unless the new
collection is with a different kit configuration, in which case it will begin in the next
available row.
• Different analyte types (protein, RNA) should be collected in different plates.
You can return to this window to access the readout files by clicking the plate icon and entering
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the plate number.
For NGS readout:
• Once finalized, plates can be moved between
readout groups and multiple readout groups can be
combined into a single readout group for processing
all together using an Illumina NGS platform.
• If needed, you can change the probe kit selection
after ROI collection and re-download the readout
package; use the new Pipeline configuration file to run
the Pipeline, upload the new DCC files and create a
new study.
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
GeoMx DSP Instrument User Manual
For nCounter readout:
• If needed, you can change the
probe kit selection after
ROI collection and re-download
the CDF and worksheet, then
create a new study in Data
Analysis. Note that the probe kit
will be applied to the entire row
with which it is associated.
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