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MAN-10152-01 for software v2.5
Identify Slides
For nCounter readout:
Protein assays: If a protein module added to the list shares the same Probe R space as
another module on the list, a warning will appear. Proceeding poses the risk of losing
data from both modules. To enable collection with both modules, upload and select a
Substitute Probe R configuration file at this step, and use Substitute Probe R in the
nCounter readout of the assay. See Appendix IV of the
Preparation User Manual
RNA assays: If adding custom RNA targets to an nCounter assay, only one custom
RNA module can be used per scan.
3. Select the appropriate Scan Type and Focus Channel for your slide.
The scan type option will be set to Fluorescence only.
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The focus channel selected must have abundant signal. FITC/525 nm (default for nuclear
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stain STYO 13) is typically used.
4. Verify the Channel Settings fields.
Ensure all channels you wish to use in the scan have been checked.
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If desired, adjust the value in the Exposure Time field, based on your empirical testing.
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If desired, adjust the name in the Biological Class field.
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5. Fill in the custom morphology field (if applicable) to populate custom fluorescence exposure
settings.
6. Select Save.
Select Next. The system will proceed to the Review Tissue Detection window.
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
(MAN-10150) for more information about Probe R.
GeoMx DSP Instrument User Manual
GeoMx DSP Manual Slide
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