nanoString GeoMx DSP User Manual page 38

GeoMx DSP Instrument User Manual
Select ROIs & Segment
Inspect the image
Ensure that the scan quality is acceptable
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for designation of ROIs and segments. Do
not proceed with collection from out of focus
images, as it can cause instrument failure.
Note that red blood cell autofluorescence is
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very common in FFPE tissues; avoid
mistaking red blood cells (which do not
contain nuclei) for nucleated cells.
Open the Scan Info menu to review scan
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parameters, date created, and other details.
Use the Full Screen control in the upper
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right corner of the scan image. Zoom in and
out using the scroll wheel on your mouse or
a pinch- zoom movement on your touch
screen or touch pad. See the gray box at
right for keyboard commands for scrolling
and zooming.
Open Render Settings
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24). Here, you can:
Change the colors used to represent
the different channels on the scan.
Adjust the intensity of each channel,
either with the slider bar or adjusting
the values in the editable Min and Max
boxes.
Use the Undo , Redo , or Revert
buttons at the bottom of the Render
Settings window, if needed.
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
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Select pointer tool
Select square tool
Select polygon tool
(see Figure
Figure 24: Scan Workspace Render Settings menu
MAN-10152-01 for software v2.5
Keyboard Shortcuts
Zoom out
Super zoom out _ (underscore)
Zoom in
Super zoom in
Pan image (arrow keys)
Super pan image Shift + (arrow keys)
Select circle tool
- (hyphen)
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