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MAN-10152-01 for software v2.5
Select ROIs & Segment
4. Click the Generate Segments button to see the segments materialize on the scan.
5. Adjust
the
value
Thresholds grid
(see
needed, to change the sensitivity to the
channel you've chosen to assess (the
channel designation set to +). Consider
starting with one ROI, adjusting the
channel thresholds until the desired
segment area is selected, then applying
that value in Override All.
Increasing the Channel Thresholds
l
number will increase the intensity
required to be included in the segment
and decrease the segment area.
Decreasing the Channel Thresholds
l
number will increase the segment area.
Adjust until the desired area is selected.
l
Record the settings to refer to when you
l
perform segmentation outside of this
practice exercise.
6. Repeat steps 2-5, selecting a different channel designation in step 2.
7. Repeat until you have noted the desired setting for each of the channels in your analysis. Use
these settings in
Segmenting ROIs in the Scan Workspace on page 46
segment definition box used for this thresholding exercise.
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
in
the
Channel
Figure 31)
, as
GeoMx DSP Instrument User Manual
Figure 31: Segments with Channel Thresholds
. Delete the
45
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