Overlays, Roi Transfer, And Export Images - nanoString GeoMx DSP User Manual

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GeoMx DSP Instrument User Manual
Overlays, ROI Transfer, Export Images
Overlays, ROI Transfer, and Export Images
Overlays
You may overlay the current scan with
imported images (including scan images
exported from other GeoMx instruments) to
facilitate the selection of ROIs.
1. Select the Overlays button in the Scan
Workspace to open the Overlays Panel
Figure
38).
2. Click the Import button and Browse to your
saved overlay image. You may import more
than one overlay.
Ensure your overlay does not exceed 32,767 pixels in width or height and is less than 3 GB in size.
PNG, JPG, and TIFF file types are supported.
3. Each imported overlay image will appear on the scan and is represented by a gray box in the
Overlays tab of the Scan Workspace panel. Here, you may rename the overlay, toggle its
visibility, set its opacity, and delete the overlay. When multiple overlays are present, drag and
drop the gray overlay boxes to change the stacking order of the overlaid images.
4. Click Alignment in the gray overlay box to reveal alignment tools on the scan
38). Alignment tools allow you to Move, Scale, Resize, Rotate, create a Mirror Image, and
Lock the overlay. Use these parameters or the alignment tools that appear on the scan to
adjust the alignment of the selected overlay on the current scan.
5. Click on the alignment tool you would like to use. There are three ways to align the overlay
using each tool.
The three "handles" on corners of the overlay. These may not be accessible if zoomed in.
l
The alignment tool in the alignment box. Use the "adjust by" field to set the increment by
l
which movements should be made.
The parameter fields in the alignment box can be incremented and edited manually.
l
Overlay modifications will be saved automatically.
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
52
MAN-10152-01 for software v2.5
(see
Figure 38: Overlays panel
(see Figure

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