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GeoMx DSP Instrument User Manual
Select ROIs & Segment
6. Click the Generate Segments button to see the segments
materialize on the scan. If you make changes to the
segment definitions, click the Save Changes button to
ensure the changes are applied to the scan.
Select whether to view/hide segments on the scan by
l
clicking on or off the eye icon
view/hide all segment/ROI masks using the Show Segments button on the ROI panel.
7. Check the Show Advanced Parameters box to open Advanced Parameters
35). Here, you can adjust numerous parameters, including the order in which segments are
generated and ultimately collected.
Erode: The border width to be eroded from each
l
segment; this effectively increases the boundary between
segments. Increasing erode decreases segment size.
N- Dilate : Adds segment area around detected nuclei.
l
This setting is only applicable for nuclear- tagged
segments.
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
48
(see Figure
34). You can
Figure 35: Segmentation advanced parameters
MAN-10152-01 for software v2.5
Figure 34: Visible segments and
hidden segments clickable icon
(see Figure
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