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MAN-10152-01 for software v2.5
Select ROIs & Segment
Open the ROIs List
(see Figure 29)
Export all ROIs as high- resolution tiled .tiff files for custom segmenting (see
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External Program for Segmenting on page
Import all custom masks created in an external program.
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Toggle all segment/ROI masks on or off with Show Segments.
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Change the ROI numbering to any unique alpha-numeric choice. (Consider preserving the
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existing ROI number at the beginning of the name in order to maintain the order of the ROIs for
collection). Do not use special characters like ' \ / * : < > | " '.? or lead with a space in the ROI or
segment names.
Manually change the dimensions or location of an ROI.
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View the nuclei count and area for each ROI and any individual segments.
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Nuclei that exist on the edge of two segments may be counted in both segments.
The displayed ROI area is calculated in micrometers whereas the segment area is based upon the
number of pixels in the segment mask. As a result, a segment that encompasses an entire ROI may
display a slightly different area than the ROI that contains it.
Add a Comment to the ROI.
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Export an individual ROI as a high-
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resolution
tiled
.tiff file
segmenting or Import a segment mask(see
above).
Delete an ROI. To delete all ROIs in the
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scan workspace, select an ROI in the
ROI List, press Ctrl+A, then press Delete or
Backspace.
View the current Well Count for this scan at
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the bottom of the ROI panel. This is the
estimated number of wells that the current
selection of ROIs and segments will occupy
in the collection plate.
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
to:
51) .
for
custom
GeoMx DSP Instrument User Manual
Figure 29: ROIs List
Using an
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