nanoString GeoMx DSP User Manual page 32

GeoMx DSP Instrument User Manual
Identify Slides
2. Select Reagent Configuration files.
GeoMx DSP probe kit configuration (.pkc) files associate GeoMx readout barcodes with RNA
and Protein assay targets. Morphology Kit configuration (.mkc) files provide visualization
target information to the GeoMx DSP Software.
Configuration files are not pre-loaded on the instrument and must be transferred using a
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USB drive or remote access over Chrome. Find the files for the probe kits used on your
slides at www.nanostring.com/dspconfigfiles. Save these files to a USB drive. Unzip files
before uploading to the instrument. Once uploaded to the instrument they are available for
selection in Scan Parameters.
Select the Morphology Reagent Kit used in preparing the slide from the drop-down. If not
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already loaded on the instrument, select the upload button to the right of this field to upload
the respective unzipped configuration file from your USB drive (see above). See
Scan Parameters on page 68
parameters.
Select the probe kits used in preparing the slide from the Probe Reagent Kit drop-down.
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The Core Kit must be selected before the compatible Module Kits will be available in the
drop-down. Select the upload button to upload new configuration files from your USB drive,
if needed. If newly uploaded files do not appear in the drop-down, exit the Scan Parameters
window, then re-open.
Enter the lot number of the probe kit(s). Your selected probe kits are listed to the right of the
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upload buttons.
Uploaded probe kit configuration files can be activated and inactivated under Kit
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Management under Administration (see
Ensure you have uploaded the correct configuration files for the core and each module used
during slide preparation. Note the following:
Failure to select a Core probe kit in the Probe Reagent Kit field will result in Scan-only
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mode (no aspirate collection).
If using a custom panel, NanoString Bioinformatics will provide a custom .pkc file. Upload
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this file to the GeoMx DSP, then select it from the Probe Reagent Kit drop-down menu,
along with any other probe kits in use in this collection.
If you do not see the file you need, contact
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To change the .pkc file associated with a slide after collection is complete, see
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a probe kit configuration file after collection on page
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
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for GeoMx Solid Tumor TME and Melanoma Morphology
Kit Management on page
GeoMxSupport@nanostring.com
MAN-10152-01 for software v2.5
87).
for assistance.
Changing
69.
Example